髓细胞的

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

The expression level of metabolitic enzyme (CYP1A1, CYP1B1) and its correlation with cell differentiation /apoptosis were studied as well. The influence of resveratrol in cell cycle was determined with FCM. Results 1 Resveratrol not only suppresses the growth of medulloblastoma cells in a dose and time related fashion but also induces cell differentiation and apoptosis. Resveratrol promotes differention of UW228-1,-2 cells to forward glia, UW228-3 and Med-3 cells to neuron. A treatment of resveratrol in the concentration of 100μM for 48hrs comit most of cells die of apoptosis. 2 Among the four cell lines Fas and Caspase-3 were constantly expessed, whereas FasL was absent irrespective to the drug treatment. 3 ICC, RT-PCR, Western-blot hybridization and EROD enzymatic activity assay demonstrated that expression of CYP1A1 is different after treatment with resveratrol in four cell lines, up-regulated in three UW228 cell lines in a dose dependent manner but down-regulated in Med-3 cells. Suppressive effect of resveratrol on CYP1B1 expression is the same among four medulloblatoma cell lines. 4 Cell cycle distribution of UW228-3 was greatly changed after treatment.

结果：1、白藜芦醇以时间和剂量依赖性方式抑制髓母细胞瘤细胞生长，同时促进细胞的分化和凋亡：白藜芦醇促使UW228-1、-2靶细胞向神经胶质细胞方向成熟分化，UW228-3、Med-3细胞向神经元细胞方向成熟分化，以100μM作用48小时效果显著；2、四个细胞系均有Fas和Caspase-3表达，但无Fas-L基因表达和蛋白活性产生，因此难以形成Fas相关性死亡通路；3、白藜芦醇处理前后CYP1A1基因的表达在各细胞系略有不同：UW228-1，-2，-3三个细胞系白藜芦醇以剂量相关性方式上调CYP1A1的表达，而Med-3细胞系，白藜芦醇却抑制CYP1A1的表达；四个细胞系在白藜芦醇处理后CYP1B1的表达均受到抑制；4、UW228-3细胞系经白藜芦醇作用后细胞周期有较大改变，表现在G0/G1期在整个细胞周期中所占的比例增加，而其它时期则相应减少。

Neural cell adhesion molecule L1, discovered in the 80s, is one of the matters that can promote nerve regeneration. It can mediate the interaction of cell-to-cell and cell-to-matrix, take great efforts on neurite outgrowth and fasciculation, migration of neural cells, formation of the myelin, structure of neural trace, transmembrane signal transduction, inhibiting apoptosis of neural cells, even on immune system and formation of the tumor. Li is discovered on the mouse's Cerebellar membrane primarily. Thereafter, it also is found in post- mitotic neurons, pre- and non-myelinating Schwann cells and so on.

神经细胞粘附分子L1（L1）是80年代发现的具有促进神经再生作用的物质，它能介导神经细胞与神经细胞之间的粘附，在神经轴突的生长、聚集，神经细胞的转移，纤维髓鞘的形成，神经通路的构建及信号的转导，抑制神经细胞的凋亡甚至在免疫系统、肿瘤的发生等方面均具有非常重要的作用。L1最初是从小鼠的小脑膜中发现，后来发现在有丝分裂后的神经元，髓鞘形成前及非形成髓鞘的雪旺细胞等组织中同样存在。

Results: The proliferation of Kasumi-l cell was obviously inhibited by VPA on dose- and time-dependent manner. The IC50 was 2.33 mmol/L treated with VPA for 72 h. Incubation with VPA, the cell ratio at G0/C1 phase increased. VPA induced myeloid cell differentiation antigen CD11b to increase with a time-and dose-dependent way and caused apoptosis. Wright's staining and light microscope were used to show the morphology of partial differentiation and obvious apoptosis with 3 mmol/L VPA for 72 hours. The typical DNA ladder of apoptosis characteristic was observed with DNA gel electrophoresis.

结果：VPA能显著抑制Kasumi-1细胞的增殖，且呈剂量和时间依赖性，VPA处理Kasumi-1细胞72h时的半数抑制浓度(IC50)为2.33 mmol/L；VPA处理后，细胞周期检测G0/G1期细胞比例逐渐增高；VPA能诱导髓系分化抗原CD11b表达水平的阳性率升高，呈时间及剂量依赖性；VPA能诱导Kasumi-1细胞凋亡，Kasumi-l细胞经VPA3 mmol/L处理72h后，出现典型的凋亡细胞所具有的梯形DNA条带。

Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562，采用MTT法检测细胞增殖活性，单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象，Annexin V FITC/PI双标法检测细胞凋亡，Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

The cases were submitted with diagnoses that included intratubular seminoma, intratubular germ cell neoplasia, unspecified, Sertoli cell hyperplasia, myeloid sarcoma, and lymphoma.

这10例原单位的诊断包括管内精原细胞瘤、管内生殖细胞肿瘤、支持细胞增生、髓细胞肉瘤和淋巴瘤。

Two types, the myelinated nerve fiber and nonmyelinated nerve fiber, the function of the neuromem

髓神经纤维。神经膜细胞和髓鞘的功能是隔离和绝缘。

Methods P-gp expression in 151 acute myeloblastic leukemia and 47 acute lymphoblastic leukemia was determined by monoclonal antibody UIC2 and flow cytometry. Results The rate of complete remission differred between P-gp+ and P-gp?

采用流式细胞术及单克隆抗体UIC2检测了151例成人急性髓细胞白血病、47例成人急性淋巴细胞白血病患者的白血病细胞P-gp表达。

Part III: Peripheral blood dendritic cells in coronary atheroscleroticdiseaseObjectives: Dendritic cells subsets, Myeloid DC and plasmacytoid DC, regulate immune reactions by polarising naive T-helper cells into a Th1 or Th2 response, respectively.

第三部分：冠心病患者外周血树突状细胞亚型的变化的研究目的：树突状细胞的两个亚型，髓样DC和浆细胞样DC分别是促使T辅助细胞向Th1和Th2免疫应答转化的抗原呈递细胞。

The major concern during laser irradiation of teeth is the high potential of tissue overheating resulting high temperatures that can spread in toward the dentin,causing irreversible damage to cells presented in the pulp chamber and leading to cell death,so the related laser irradiation parameters should be controlled strictly.

激光辐照牙齿时所产生的温升容易引起牙髓细胞不可逆的损伤，导致牙髓细胞坏死，因此必须对相关的激光参数加以控制。建立了激光与牙齿相互作用的热传输模型，利用Crank-N icolson有限差分法数值模拟了不同Nd∶YAG激光参数照射下牙齿表面及内部的温度场的分布。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。