髓细胞的

Objective:To study the effect of the centrifugal force on the constructing of tissue engineering nucleus pulposus.

目的：探讨离心应力对髓核细胞功能表达和组织工程髓核结构的影响？

After disposal,schwann cells, myelin sheath and axon of nerve fibers were eliminated,but the basement membrane tube of schwann cells and three-dimensionalsupports of extracellular matrix of epineurium and perineurium werereserved.

经脱细胞处理后，去除了Schwann细胞、神经纤维的髓鞘和轴突，保存了由Schwann细胞基底膜管以及神经外膜和束膜的细胞外基质构成的三维支架结构。

The many dark clusters of cells in the hepatic parenchyma are islands of extramedullary hematopoiesis typical for fetal liver.

在肝实质有许多细胞密集排列成簇，这些细胞是髓外具有造血能力的肝细胞岛。

Methods: Nonadherent cells from bone marrow or peripheral blood mononuclear cells of 8 patients with chronic myeloid leukemia were cultured with rhGM- CSF and rhIL- 4. The cytokines were supplemented every three days. RhTNF-α was used at the ninth day. All the cells were harvested at the twelfth day.

从8例慢性髓性白血病患者的骨髓或外周血中获取非贴壁细胞，利用细胞因子(rhGM蛳CSF、rhIL蛳4、rhTNF蛳α)联合培养，每3 d补充新的细胞因子，其中rhTNF蛳α为第9天添加，第12天收获细胞。

To test the hypothesis that this resistance is a consequence of these cells developing a stress tolerance phenotype to survive in this milieu , we created osmotically tolerant cells hypertonic HT cells by gradually adapting murine inner medullary collecting duct 3 cells to hyperosmotic medium containing NaCl and urea .

为了检验这一假设这种阻力的后果是这些细胞发展中国家应力宽容型生存在这个背景下，我们建立了 osmotically 宽容细胞逐渐适应小鼠内髓集合管3细胞高渗培养基NaCl和尿素。

I.. The virions were observed in the cylindrical cell at 9 h p. i.. The intact virions probably penetrated through the peritrophic membrane, then absorbed and invaded the microvilli for infecting the cylindrical cells.

作者认为病毒入侵是以整个病毒粒子穿越中肠围食膜，吸附并进入微绒毛，感染圆筒形细胞，与前入病毒入侵只是髓核进入细胞，而病毒衣壳仍留在细胞外的推测不同。

The virions were observed in the cylindrical cell at 9 h p.i.. The intact virions probably penetrated through the peritrophic membrane,then absorbed and invaded the microvilli for infecting the cylindrical cells.

作者认为病毒入侵是以整个病毒粒子穿越中肠围食膜，吸附并进入微绒毛，感染圆筒形细胞，与前人病毒入侵只是髓核进入细胞，而病毒衣壳仍留在细胞外的推测不同。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组)，HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显，在第三组二者的变化大致呈平行关系；在第四组基本呈反向变化关系。

Fig 1 At the experimental side, two weeks after operation (HE ×100) Many pieces of cartilage cells and spindle-shaped mesenchymal cells were found, by light microscope Fig 2 At the experimental side, twelve weeks after operation (HE ×100) Medullary cavity had formed, which contained hemoblasts and adipocytes, but the trabculae was not typical, by light microscope Fig 3 At the experimental side, twenty-four weeks after operation (HE ×100) The typical cancellous bone had formed, by light microscope Fig 4 At the control side, twenty-four weeks after operation (HE ×100) A large part of CXB was degraded, resorbed and replaced by fibrous tissue, but there was'not any new formed bone, by light mocroscope

图1 实验侧术后2周(HE ×100)镜下可见很多软骨细胞条索和团块生成及大量梭形间充质细胞图2 实验侧术后12周(HE ×100)镜下可见有髓腔形成，内含有造血和脂肪细胞，骨小梁尚不典型图3 实验侧术后24周(HE ×100)镜下可见形成典型的松质骨结构图4 对照侧术后24周(HE ×100)镜下可见大部分CXB被降解吸收，为纤维组织替代，无新骨形成

There was significant difference in signal intensity between the cell concentration group and unlabelled cell group during 2 weeks. And significant difference occurred in the 5×105 SPIO-labeled cells per milliliter medium group and unlabelled cell group during 3 weeks.3. In kidneys of ischemia-reperfusion injury,the labeled BMSCs were demonstrated as signal intensity loss in renal medulla on T2*WI sequence in days 1,3,and5.The signal intensity loss was visualized up to 8 days after transplantation,and the signal intensity in the third day was the lowest. Histological analyses showed that most Prussian blue staining-positive cells were well correlated with the area where a signal intensity loss was observed in MRI.

在此细胞浓度的基础上，标记后细胞信号在T2*WI序列的降低在2w后失去统计学意义；而在细胞浓度为5×105/ml时，标记3w后信号在T2*WI序列的降低才失去统计学意义。3缺血再灌注肾损伤家兔细胞移植后第1、3、5天肾脏髓质区信号强度明显下降，至移植后第8天信号改变不明显，其中第3天信号改变最为明显；组织学分析见绝大多数普鲁士蓝染阳性标记细胞分布于肾小管周围，与MRI信号强度下降区域基本一致。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。