髓形成

The calcium hydroxide cement containing BEMA or EMA and HEMA as resin matrix has good properties. The pulp capping test showed that an ex...

以BEMA或EMA和HEMA为树脂基体材料，凝固后具较好的整体性能，在狗牙上盖髓70天后，可见牙髓组织内形成完整的牙本质桥，显示出良好的盖髓效果。

Neural cell adhesion molecule L1, discovered in the 80s, is one of the matters that can promote nerve regeneration. It can mediate the interaction of cell-to-cell and cell-to-matrix, take great efforts on neurite outgrowth and fasciculation, migration of neural cells, formation of the myelin, structure of neural trace, transmembrane signal transduction, inhibiting apoptosis of neural cells, even on immune system and formation of the tumor. Li is discovered on the mouse's Cerebellar membrane primarily. Thereafter, it also is found in post- mitotic neurons, pre- and non-myelinating Schwann cells and so on.

神经细胞粘附分子L1（L1）是80年代发现的具有促进神经再生作用的物质，它能介导神经细胞与神经细胞之间的粘附，在神经轴突的生长、聚集，神经细胞的转移，纤维髓鞘的形成，神经通路的构建及信号的转导，抑制神经细胞的凋亡甚至在免疫系统、肿瘤的发生等方面均具有非常重要的作用。L1最初是从小鼠的小脑膜中发现，后来发现在有丝分裂后的神经元，髓鞘形成前及非形成髓鞘的雪旺细胞等组织中同样存在。

Histological structures of bursas of Fabricius were observed at the different developmental stages from chicken embryoes to chickens. The results were as follows. The bursa of Fabricius began to develop on the 13th day of post-hatching and its cell aggregated into the nodosity clique underneath the epithelium mucosae. On the 15th day of post-hatching, those cell cliques were surrounded by the flat epithelium and formed the basic structure of folliculus lymphaticus. The number of lymphocytes in the folliculus lymphaticus increased gradually, and the medulla and cortex of a majority of folliculus were not discriminated obviously. Until on the 7th day for chickens, the medulla and cortex of folliculus formed completely and the number of folliculus increased significantly. From 21 to 35 days old, the folliculus lymphaticus transformed from polygon into rectangle. Mucosa epithelial cells increased gradually and their cytoblasts arranged compactly into aline in the basilar part on the 35th day.

本研究观察了从鸡胚到出壳后的不同生长发育阶段法氏囊的组织结构，结果表明：胚胎13日龄时，法氏囊开始发育，粘膜上皮下形成结节状细胞集团；胚胎15日龄，聚集的细胞集团形成初始的淋巴滤泡；胚胎18日龄到出壳后1日龄，滤泡内淋巴细胞数量增多，多数滤泡髓质和皮质界限仍不明显；7日龄雏鸡法氏囊髓质皮质分界清晰，滤泡数量显著增多；21日龄到35日龄，滤泡由多边形逐渐转变成长方形；在35日龄时，粘膜上皮细胞逐渐增多，排列紧密，细胞核紧密排列成直线位于基底部。

Nodes of Ranvier; saltatory conduction; Schwann cells; oligodendrocytes; myelin basic protein; demyelination; remyelination; myelinopathy; myelinogenesis; myelin-associated protein; myelin sheaths; demyelinating neuropathy; myelin proteolytic protein; chronic inflammatory demyelinating polyradiculoneuropathy; multiple sclerosis; MS

郎飞氏结；跳跃传导；施旺细胞；少突神经胶质细胞；髓鞘碱性蛋白脱髓鞘；髓鞘再生；髓鞘质病；髓鞘形成；髓磷脂-关联的蛋白质；髓鞘；脱髓鞘神经病；髓磷脂溶解蛋白的蛋白质；慢性炎症性脱髓鞘多神经根神经病；多发性脑硬化；MS

To clone new disease gene of CMT, many myelin and myelin-related gene had been analysised by homologue searching. Quaking mouse, one of the mutation mouse with the clinic feature similar to Trembler mouse (the gene mutation mouse of PMP22) with the characterization of demylination in both the central and the peripheral nervous system, its disease gene had just been cloned accession No.

为进一步克隆新的CMT致病基因，在分析出CMT已克隆的基因均为与髓鞘有关的基因后，通过筛选有关髓鞘基因，我们发现，quaking基因突变小鼠因髓鞘形成障碍而出现震颤，其中枢和周围神经均有脱髓鞘，类似于PMP22基因突变鼠"Trembler"的临床表现。

The arrangement of odontoblasts was found in line and there was no inflammation. The root pulp and periodontal tissue were normal. Finally the opened area was closed by dentinal bridge.

周边造牙本质细胞排列整齐，牙髓无炎症及变性，根髓及牙周组织正常，最终形成牙本质桥将穿髓孔封闭使牙髓保存了活力。

The results of study show:1. White pulp and red pulp in parenchyma of spleen of embryo can be obviously discerned after 18 days. Periarterial lymphoid sheath and ellipsoid periarterial lymphoid sheath also can be obviously discerned in spleen of 4 days chicken. T, B lymphocytes in appendix basement of embryo emerge after 20 days. It is the initial shape of cecal tonsil. Crypt structure of conjunction of esophago and stomachus glandularis form obviously at 4 days. It is the initial shape of esophago tonsil. The germinal center firstly emerges in these three organs at 14 days. With the increase of day age, the characteristic structure peripheral immune organs gradually develop mature. Spleen achieve mature at 21 days and cecal tonsil at 35 days.2. IgM~+ and IgA~+ cells in spleen of embryo emerge at 15 days. IgG~+ cell, CD3~+ and CD8~+T lymphocytes of embryo emerge at 20 days. CD3~+, CD8~+ and IgM~+ cells in cecal tonsil of embryo emerge at 20 days. However CD4~+, IgG~+ and IgA~+ cells all emerge in 1 day age chicken out of crust. CD3~+, CD4~+, CD8~+, IgM~+ and IgG~+ cells in esophago tonsil of embryo all emerge at 20 days. However IgA~+ cells emerge in 1 day age chicken out of crust.3. The amount of T, B lymphocytes in peripheral immune organs increase follow with the increase of day age, and hold an upgrade tendency. The amount of T, B lymphocytes in spleen achieved stabilization at 21 days, and in tonsil of esophago and appendix at 35 days.

研究结果表明：1、在组织结构方面，脾脏实质内的白髓与红髓在胚胎18日龄后明显可辨，4日龄雏鸡脾脏中形成明显可辨的动脉周围淋巴鞘和椭球周围淋巴鞘；盲肠基部T、B淋巴细胞在胚胎20日龄时开始出现，即初步形成盲肠扁桃体；食管与腺胃结合处在4日龄时形成明显的隐窝结构，即食管扁桃体初步形成；14日龄时，三种器官中首次出现生发中心；随着日龄的增长，外周免疫器官特征结构不断发育成熟，脾脏在21日龄时达到成熟水平，盲肠扁桃体和食管扁桃体在35日龄时达到成熟水平。2、在T、B淋巴细胞出现时间方面，脾脏中IgM~+和IgA~+细胞在胚胎15日龄时开始出现，IgG~+细胞、CD3~+和CD8~+T淋巴细胞在胚胎18日龄时出现，CD4~+细胞在胚胎20日龄时出现；盲肠扁桃体中CD3~+、CD8~+和IgM~+细胞在胚胎20日龄时开始出现，而CD4~+、IgG~+和IgA~+细胞均在雏鸡出壳后1日龄时出现；食管扁桃体中CD3~+、CD4~+、CD8~+、IgM~+和IgG~+细胞均在胚胎20日龄时开始出现，而IgA~+细胞则在雏鸡出壳后1日龄时出现。3、在T、B淋巴细胞数量变化方面，外周免疫器官中T、B淋巴细胞的数量随日龄增长，整体均呈上升趋势。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Methods:Compounding plup-capping agents consisted of erythromycin,metronidazole indomethacin mixed with CPC.Compounding plup-capping agents as direct plup-capping agents were used in 78 exposed plup cases as experitmetal group. Pure calcium hydroxide were in 51 exposed plup cases as controls.

在磷酸钙骨水泥中添加红霉素、甲硝唑、消炎痛形成一个消炎、止痛的药物缓释系统和具有良好生物相容性的盖髓剂，对实验组78例露髓者行直接盖髓，对照组51例用氢氧化钙盖髓，进行临床研究。

The sinus gland is a roughly ellipsoidstructure which is situated dorso-laterally at the transition of the medulla intema andmedulla terminalia.

这些神经分泌细胞发出的轴突汇集成束，形成一条轴路，斜穿过端髓，在端髓与内髓交接处背侧膨大形成窦腺。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。