髓原细胞

METHODS: Detection of the cell proliferation by MTT method and ALP expression by enzyme dynamics method.

原代培养牙髓成纤维细胞、牙周膜成纤维细胞，用MTT法测定细胞增殖，酶动力学方法测定ALP活性。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

Results Osteoclast and osteoblast around trabecula of bones showed proliferation, shi1e fatty tissues in bone marrow decreased and hematopoietic tissue well-regenerated alter the hyperbaric oxygen-treated. Also juvenal osteocyte and collagen fibril were observed in early stage by electron microscope.

结果 多数骨小梁周围可见骨母细胞增生、偶见破骨细胞增生，髓腔内脂肪细胞逐渐减少，造血组织增生；电镜发现骨小梁内早期即出现幼稚骨细胞和胶原原纤维合成。

The females incubated at 33 , their ovarium has powerful cortex, the germinal epithlium produce oocyte, its medulla degenerate into ovarian cavity. During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of ovary increase in quantity, they disintegrate after sexual differentiation. There are a lot of big grana in cells of ovary before sexual differentiation, in the course of sexual differentiation the quantity of the big grana decrease, while small grana increase and peakafter sexual differentiation. The males incubation at 26 , cortices of their testes degenerate into a thin albuginea. Medulla are formed by testicular cords in which scatter spermatogonium.

33℃下孵化的小龟卵巢皮质发达，由原始生殖细胞产生卵原细胞，髓质退化成为卵巢腔，分化时卵巢细胞的超微结构显示线粒体和内质网的数量大大增加，它们在分化结束后解体，分化前细胞内有较多的大颗粒，在分化中大颗粒减少，小颗粒增多，在分化后小颗粒达到最多。26℃下孵化的小龟精巢皮质退化成为一层薄的白膜，髓质有许多睾丸管构成，管腔内有散落排列的精原细胞。

E studied expression levels of WAVE1 in six leukemia cell lines (the human Jurkat T leukemia cells, U937 histiocytic lymphoma cells, Burkitts lymphoma cell Raji, acute promyelocytic leukemia cell HL-60, chronic myelogenous leukemia cell K562 and K562/A02) by Western blotting analysis. Levels of WAVE1 expression were high in all six human leukemia cancer cell lines. In contrast, the constitutive expression levels of WAVE1 were noticeably lower in normal human peripheral blood mononuclear cells, or non-blood cancer cell-lines, including human lung A549 cancer cells, Hela cervical cancer cells, MG-63 osteosarcoma cells, CNE2 nasopharyngeal carcinoma cells, human umbilical vein endothelial cell, QSG7701 hepatocarcinoma cells, and WI-38 lung fibroblastoma cells.

AVE1在人类血液肿瘤细胞系(人T细胞白血病细胞系Jurkat、人组织细胞淋巴瘤细胞系U937、人Burkitts淋巴瘤细胞系Raji、人原髓细胞白血病细胞系HL-60、人慢性髓原白血病细胞系K562和K562／A02)中高表达，在非血液肿瘤细胞系(人肺腺癌细胞系A549、人宫颈癌细胞系Hela、人成骨肉瘤细胞系MG-63、人鼻咽癌细胞系CNE2、人脐静脉内皮细胞系HUVEC、人肺成纤维细胞系WI-38、人肝上皮细胞系QSG7701)以及正常人外周血单个核细胞中低表达或不表达。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

The cases were submitted with diagnoses that included intratubular seminoma, intratubular germ cell neoplasia, unspecified, Sertoli cell hyperplasia, myeloid sarcoma, and lymphoma.

这10例原单位的诊断包括管内精原细胞瘤、管内生殖细胞肿瘤、支持细胞增生、髓细胞肉瘤和淋巴瘤。

Results showed that urokinase at 20U/ml was the optimal concentration used. Fibrinolysis kinetics and plasma plasminogen concentration of 27 normal persons and 27 patients with acute myeloblastic leukemia were detected, a significant difference was observed between the two groups. Repetition test showed that the coefficience of variation was 5.24±1.51%. This method could also be used to detect the concentration of plasma fibrinogen.

结果 初步建立了正常人纤溶动力学分析参数，确定了20U/ml尿激酶为最适试验浓度，检测22例正常人，27例急性髓细胞白血病患者纤溶动力学及纤溶酶原含量，显示患病组较正常组差异有显著意义(P.05， P.01)，重复性试验显示该方法的变异系数为(5.42±1.51)%，并可同步测定血浆纤维蛋白原浓度。

Methods: The intervertebral disc cells were isolated from the nucleus pulpous of lumbar intervertebral discs of New Zealand white rabbits.

目的：研究人骨形态发生蛋白2BMP-2）对体外培养兔腰椎间盘髓核细胞Ⅱ型胶原和aggrecan的影响。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间，进行髓核细胞活力测定；爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察；MTT 法绘制髓核细胞生长曲线，并行原代及第2 代细胞透射电镜观察，对体外细胞的生物学特性进行研究。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。