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Objective:To investigate the relationship of the expression of phosphorylation p38 mitogen activated protein kinase(p-p38MAPK) and the inflammatory damage of dorsal root ganglion and neuropathological pain caused by non-compressed nucleus pulposus in rats.

目的:观察背根神经节磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)表达的变化与非压迫性髓核所致的炎性反应及病理性神经痛的关系。

Methods:IL-6 expression in 8 samples of normal and 10 of inflammatory pulpal tissue was examined using immunohistochemical techniques.

采用免疫组化法,检测正常组8个牙及炎症牙髓组10个牙中IL-6的表达。

Results:Negative staining of IL-6 was found in all the cases of normal pulpal tissue while positive in all inflammatory pulp.

采用免疫组化法,检测正常组8个牙及炎症牙髓组10个牙中IL-6的表达。

In control mice, mild intensity of immunoreactive staining of phosphorylated ERK1/2 was seen in the neurons of the marginal zone and the substantia gelatinosa in the caudal subnucleus of the trigeminal spinal nucleus (Sp5C).

免疫组织化学的观测表明,正常小鼠延髓尾侧亚核Ⅰ和Ⅱ层的神经元存在微弱水平的ERK1/2活性,左右侧无明显区别;尾侧亚核神经元存在高水平的磷酸化CREB。

After embeding routingly,serial 10μm sections were prepared for modified trichromic staining with improve-ments to manifest axons and myelin sheaths simultaneously,immunohistochemistryand in situ hybridization.

组化染色用新的改良三色法同时显示神经轴突和神经髓鞘。

Methods The animal model of skin avulsion injury was replicated, and the expression of CD18mRNA in avulsed skin tissues was detected by RT-PCR method; and the amount of neutrophilic granulocyte in tissues was detected by MPO method in order to analyze the tendency of neutrophil of avulsed tissues after trauma.

复制猪皮肤撕脱伤模型,采用RT-PCR方法检测伤后不同时间撕脱组织中CD18mRNA的表达;用髓过氧化酶(Myel operoxi daBe enzyme,MPO)法测定组织中中性粒细胞数量,研究伤后撕脱组织中中性粒细胞的趋化情况。

A total of 60 male Sprague-Dawley rats were randomly divided two groups, each group was further subdivided into sham, control, SS group (n=6, per subgroup), animas were sacrificed at different reperfusion periods of 2 h and 24 h, respectively. Oxygenation indexes, lung injury scores, wet/dry ratio, myeloperoxidase activity, Malondialdehyde and cytokine-induced neutrophil chemotactic factor in the transplanted lung and serum were measured.

雄性SD大鼠60只,随机分2组,每组又分3个亚组(n=6):假手术组、0.9%氯化钠液对照组、西维来司钠组,分别在冷缺血4 h后再灌注2 h和24 h收集标本,测量移植肺氧合指数(PO2/FiO2)、肺损伤病理评分、移植肺湿干重比、髓过氧化物酶活性(MPO、肺组织和血清中丙二醛、细胞因子诱导的中性粒细胞趋化因子。

The expression of c-fos protein in the nucleus of tractus solitarius and dorsal motor nucleus of vagus of the central bulb was observed by immunohistochemical method.

分别于刺激后0.5、l、2和5h处死大鼠,以免疫组化方法观察c-fos蛋白在延髓孤束核和迷走神经运动背核中的表达。

It's regarded as a dentin specific protein, The gene structure and the expression pattern of DSPP at transcript level are well known in recent years, however, the expression pattern of DSPP protein, and it's possible role during tooth development and dental pulp reparation after injury are not clear yet.

近年来,DSPP的基因结构及mRNA水平的表达模式已较清楚。蛋白印迹实验证实小鼠牙胚中有DSPP蛋白的存在。但是,DSPP在蛋白水平的表达模式如何?它的生物学功能和作用机制是什么?它在牙髓损伤修复中的作用又如何?目前国内外均未见报道。这些问题的研究,对我们在该蛋白后基因水平的了解具有重要意义,也为我们深入了解牙本质生物矿化机制、对牙体组织疾病和损伤进行生物性防治提供理论依据。

After harvesting the preselected discs, tissue samples were obtained from the center of the nucleus pulposus and the middle zone of the anulus fibrosus.

在取出预定的椎间盘有,我们在其中的髓核中心和纤维化的中间得到了组织样本。

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