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Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562,采用MTT法检测细胞增殖活性,单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象,Annexin V FITC/PI双标法检测细胞凋亡,Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

CHF-associated diseases were Caroli's syndrome, polycystic kidney disease, caernous transformation of the portal ein, Joubert's syndrome, on Meyenburg complex, polydactyly, medullary sponge kidney, and pancreatic duct atrophy.

先天性肝纤维化相关的疾病包括Caroli's综合症、多囊肾、海绵状门静脉形成、Joubert's综合症,on Meyenburg 复合物,多指趾畸形,髓状海绵样肾,胰管萎缩。2例发展成胆管癌。

Objective To study the possibility of using radiopaque resinifying agent in resinification of the tooth.

雍 王嘉德目的为研究显影塑化剂在牙髓塑化治疗的显影效果。

Samples were procured at 6 week after transplantation and studied by nerve silver staining and myelin basic protein histochemical staining and nerve growth factor receptor immunohistochemical staining.

6周后组织切片,行 HE染色、嗜银染色以及髓鞘碱性蛋白和抗神经生长因子受体抗体免疫组化检查。

Such massive splenomegaly is usually indicative of some myeloproliferative disease such as chronic myelogenous leukemia or myelofibrosis.

这样的脾肿大通常预示有骨髓增生型疾病,例如慢性髓性白血病或骨髓纤维化。

Such massive splenomegaly is usually indicative of some myeloproliferative disease such as chronic myelogenous leukemia or myelofibrosis .

这样的脾肿大通常预示有骨髓增生型疾病,例如慢性髓性白血病或骨髓纤维化。你知道它的英文吗?

There was no statistics difference between antegrade nail and retrograde nail.

按照盲化对象的不同盲签名可划分为盲消息签名和盲参数签名。9。顺行与逆行髓内钉之间差异无统计学意义P=0。

The mice were sacrificed on 7, 10, 14, 21 and 28 days post-inoculation and then the hearts were aseptically removed. Pathological changes in the myocardium were observed by light microscopy and the expression of the CD4+ T lymphocyte in the myocardium was determined by immunohistochemistry. ResultsThe CD4+ T lymphocyte was found in the myocardium in the CVB3 infection group.

分别于接种病毒后第7、10、14、21、28d随机取正常对照组小鼠3只、病毒感染组小鼠8只断髓处死,无菌条件下取心脏,光镜下观察心肌组织的病理变化并计算心肌病理组织学积分,应用免疫组化方法检测CD4+ T细胞在心肌中的表达,并与心肌病理组织学积分作相关性分析。

METHODS: Immunohistochemical staining was adopted for studying the expression of CaB in healthy and carious teeth, and the results were analyzed by image analysis.

用免疫组化ABC法观察CaB在健康和龋坏人恒牙牙髓中的表达,并用图像分析系统进行半定量分析。

Methods: Immunohistochemical technique was applied to detect the expression and distribution of Smad2 and 3 proteins in human normal (15 cases),carious (20 cases) and inflammatory(10 cases) pulp tissue.

用免疫组化方法检测Smad2、3在正常、龋坏及炎症牙髓组织中的表达。

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