髓化

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

This rock contained chalcedonic cement before it underwent diagenesis, a process where low temperatures and low pressures alter the content of sediments before they finally lithify, or turn into rock.

这块石头包含石髓的水泥之前，它经历了岩化作用，一个过程，那里的低温和低的压力，改变的内容，沉积物之前，他们终于岩化，或变成岩石。

Methods: 50 normal Wistar male rats were randomly divided into five geoups: the sham operated group, injury model group, shenjindan capsule treat group, high dose and low dose group (duzhongyaotong pill treat, D and E)(n=10each). Autogenous nucleus pulposus was removed from the coccygeal vertebral and placed into the back of left L5~6 nerve root, The modle of the non-compressive nucleus pulposus protrusion was created by transplanting autogenous nucleus pulposus in the back of left L5~6 nerve roots. At tow weeks, the SP in dorsal root ganglion was determined by immunohistochemistry.

取健康Wistar大鼠50只，随机分为假手术组、模型组、对照组、高剂量组（D组、腰腿理痛散高剂量组）、低剂量组（E组、腰腿理痛散低剂量组），每组10只，将大鼠自身的尾椎髓核取出移植于左侧硬膜外腔L5、L6神经根背侧，造成非压迫性大鼠自体髓核移植硬膜外模型，通过免疫组化的方法测定大鼠2周时DRG中CGRP的变化。

After fixation,the brain was cut coronally into four blockes:the middle partof cerebrum,midbrain,pons and medulla oblongata.These blockes were embeddedroutingly and serial 8μm sections were prepared for HE,specific histochemical andimmunohistochemical stainings.

所有脑组织经固定后冠状切取大脑中部、中脑、桥脑及延髓4个组织块，常规包埋，连续切片（8μm）分别作HE、组化及免疫组化染色。

The result showed that the exogenetic TGF-βhad significantly inhibiting effect on both LPS's restraining the proliferation of pulp cell and inducing the apoptosis of pulp cell.

为探讨TGF-β对LPS介导的人牙髓细胞损伤修复的调控机制，首先采用了免疫组化染色技术检测了人牙髓细胞内源性TGF—β的表达变化。

The signal intensity characteristic of MRI associated with brain myelinization is helpful to differentiate the normal and abnormal brain maturation.Also,T1WI is much more useful in the detection of immature myelin and in monitoring of normal brain development in the first 6 months of life.On the other hand,T2WI is more useful after 6 months of life.

不同月龄段不同解剖部位正常脑髓鞘化具有各自MRI信号特征，有利于评判是否脑发育正常和患脑白质病，T1WI评估未成熟髓鞘较好，T2WI确定髓鞘是否成熟较好。

Results showed sTGC-CM, which is similar with the effect of hTGC-CM, was able to induce human DPSCs to make odontoblastic changes as indicated by remarkable morphological changes, increased proliferation ability, higher multipotential and colony forming capability, and expression of some odontogenic markers in gene and protein levels,such as DSP, DSPP, DMP1, BSP, OCN, OPN.

结果表明：人牙髓干细胞经过一定时间的猪牙胚条件培养基诱导后，能在细胞形态上和细胞周期上发生改变，能具有更高的克隆形成能力和多向分化能力，能维持更好的干细胞特性，具有更强的增殖能力，表达更高水平的碱性磷酸酶，免疫组化和RT-PCR提示诱导后的牙髓干细胞能在蛋白水平和基因水平表达出一些牙向分化的标志，如DSP、DSPP、DMP1、BSP、OCN、OPN等。

Results: The Fos-like immunoreactive neurons distributed mainly in the spinal trigeminal subnucleus caudalisipsilaterally and medullary visceral zone (MVZ,including nucleus tractus solitarius,ventrolateral medulla and the reticular formation between the first two),ln the sections processed for double staining of Fos and TH immunohisto chemistry, numerous Fos-LI neurons showed also TH- like immunoreactivity in their cytoplasm(Fos-TH cells)in MVZ.

结果：Fos样免疫反应神经元主要分布在同侧三叉神经脊束核尾侧亚核、双侧延髓内脏带(孤束核、腹外侧区及二者之间的网状结构)。在抗Fos 和抗酪氨酸羟化酶的双重免疫染色切片上，见到延髓内脏带内许多Fos阳性神经元的胞浆同时呈TH样免疫反应(Fos-TH双重阳性细胞)。

The annulus is richly innervated with painfibers, and further degeneratinon tends to be associated with more frequent and more intense episodes of pain.

无痛期间的特征是固定和僵凝，变性持续下去，纤维环和髓核的胶原结构发生变化并以纤维化及髓核碎裂告终。

The neural structural relations between the chemosensitive areas of the ventrolateral medullary surface and the intramedullary nuclei were investigated by local applications of horse radish peroxidase to different parts of the chemosenstive areas separately.

采用辣根过氧化物酶组化方法对延髓腹外侧表面化学感受区与延髓内部区域之间是否存在神经结构的联系进行了探查。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。