骨质的

OBJECTIVE: BMSCs from osteoporosis rats were isolated and cultured in vitro. To observe biological features of BMSCs of rat models of osteoporosis, to analyze cytopathology mechanism of osteoporosis, and to provide a significant drug target for prevention and treatment of osteoporosis.

目的：对骨质疏松大鼠骨髓间充质干细胞进行分离和体外培养，观察骨质疏松模型大鼠骨髓间充质干细胞的生物学特性，分析骨质疏松的细胞病理学机制，以期为骨质疏松的防治提供一个有意义的药物靶标。

The mechanical strain induces the up-regulation of expression of integrin α2、β1、β3 on the membrane of osteoblasts from 3control group and promoted prolifration and differentiation of osteoblasts from 3control group at 400μstrain. The expression of integrin α2、β1、β3 and Doctorship Thesis of Sichuan University------Abstractsynthesize function of bone matrix in osteobla

上述实验结果提示：16月龄大鼠复制成年人雌激素缺失骨质疏松的模型优于3月龄大鼠骨质疏松模型。2力学刺激可引起正常大鼠和骨质疏大鼠成骨细胞膜表面integrinα2、β1、β3表达

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

Results The representation of the structures of ill ears on CTVE and its comparison with coronal and axial CT film and operation: In 24 purulent otitis media, CTVE had a coincidence of 91.7% with operation of handle of malleus, 95.8% of head of malleus,95.8% of long limb of incus ,100% of short limb of incus, 87.5% of limbs of stapes.

结果：24耳慢性化脓性中耳炎中CTVE显示锤骨柄、砧骨长脚骨质破坏与手术所见的符合率为91.7％（22/24），显示锤骨头骨质破坏的符合率为95.8％（23/24），显示砧骨短脚骨质破坏的符合率为100%（24/24），显示镫骨脚骨质破坏的符合率为87.5％（21/24）。

Investigation for morbidity of osteoportic fracture (1) Osteoporotic hip fracture was the most osteoporotic fracture type in hospital.The female patients is more than male.(2) There were lots of osteoporotic fracture cases from 65 to 79 years old people. Quantity of osteoporotic vertebral compression fracture was still highly.(3) There were no famous seasonal different of morbidity among all kinds of osteoporotic fracture patients. But there were famous differences in osteoporotic hip fracture. Especially, there were many osteoporotic hip fracture cases in northern area in spring, but in winter in souther area in China.

骨质疏松性骨折发病率初步调查(1)骨质疏松性髋部骨折是骨质疏松性骨折住院治疗最多的骨质疏松性骨折类型，老年女性发病比男性多；(2)骨质疏松性骨折高发年龄在65～79岁之间，骨质疏松性脊柱骨折发病率仍然很高，应引起重视；(3)骨质疏松性椎体骨折发病无明显季节性；但骨质疏松性髋部骨折的发病在不同地区与季节有一定关系，北方春季和冬季高发，南方冬季和夏季高发。

Results Male thickness of rubbed sclerotin at frontal protuberance, geison, arcus jugalis , mandibular angle and external occipital protuberance were 5.30±1.24, 2.33±0.24, 2.84±0.75, 6.25±0.75, 8.47±1.36mm; female thickness were 4.20±1.10, 2.10±0.15, 2.14±0.46, 5.10±0.45 and 6.73±1.10mm.

结果 男性额结节可去的骨质厚度5.30±1.24mm，眉弓部可去的骨质厚度2.33±0.24mm，颧弓可去的骨质厚度2.84±0.75mm，下颌角可去的骨质厚度6.25±0.75mm，枕外隆凸可去的骨质厚度8.47±1.36mm，女性分别为：额结节可去的骨质厚度4.20±1.10mm，眉弓部可去的骨质厚度2.10±0.15mm，颧弓可去的骨质厚度2.14±0.46mm，下颌角可去的骨质厚度5.10±0.45mm，枕外隆凸可去的骨质厚度为6.73±1.10mm。

FSN can raise AA rats low LTT of spleen to normal nearly, remedy the disorder of Th/Ts and Th1/Th2 cells balance in peripheral blood, restrain exorbitant TNF-α、IL-1 produced by PMφ, thereof exert anti-inflammatory and immunoregulation effect.(3) Inside the lesion joints, FSN can depress abnormal hyper-expression of TNF-αmRNA and NF-kB in synovial tissues, as well as advance the expression of Caspase-3 (a proteolytic enzyme of apoptosis), reduce synovial immflammation and proliferation.(4) FSN can lower the expression of VEGF in synovial tissue, reduce neogenetic veins, so inhibit the growth of pannus tissue and the damage of cartilage and bone by that.(5) The above effect of FSN are in proportion to its dosage. Conclusions: FSN has inhibitory effects on symptom and condition of experimental RA, which is better than TWP as a whole.

结果：(1)FSN有明显的抗炎作用，可显著抑制AA大鼠足跖肿胀与多关节炎，改善大鼠的全身情况，同时明显减轻病变关节滑膜炎症与增生，防止关节软骨及骨质的破坏，其综合作用优于TWP；(2)FSN还能使AA大鼠低下的脾LTT恢复至接近正常，纠正外周血中T细胞亚群Th/Ts及Th1/Th2平衡紊乱，抑制大鼠PMφ过高的TNF-α、IL-1分泌，而发挥抗炎和免疫调节作用；(3)在病变关节局部，FSN能显著抑制AA大鼠滑膜细胞异常增高的TNF-αmRNA的表达，降低滑膜组织中NF-kB表达，并增加凋亡蛋白酶Caspase-3表达，从而抑制滑膜的炎症与增殖；(4)FSN还可下调滑膜组织中VEGF表达，减少血管新生，由此抑制血管翳的形成及其对软骨和软骨下骨的侵蚀；(5)FSN的上述作用皆呈现一定的量效依赖关系，高剂量组作用优于低剂量组。

For normal adult male rat,calcitonin depresses the osteoclastic resorption obviously through reducing the number of active resorptive surface and depressing osteoclastic activity;on the other hand,calcitonin depresses the osteoid producing activity of osteoblasts by indirectly affecting the coupling process between resorption and formation.Calcitonin administered in short did not affect the bone volume.

降钙素对正常成年雄性大鼠松质骨而言，可以明显抑制破骨吸收，这种作用可能是通过减少活性吸收表面数量和降低破骨细胞的吸收功能来完成的；可以抑制成骨细胞形成类骨质的活性，这种作用可能不是直接的，而是通过降钙素对骨吸收的抑制作用进而影响了骨吸收与骨形成的之间的偶联而完成的；短期应用降钙素骨量无变化。

Serologic screening for celiac disease was positive in 12 (4.5%) of 266 patients with osteoporosis and in six (1.0%) of 574 patients without osteoporosis. All but two serologically positive individuals underwent intestinal biopsy, which was positive in nine osteoporotic patients and in one nonosteoporotic patient. The prevalence of celiac disease confirmed by biopsy was 3.4% among the osteoporotic population and 0.2% among the nonosteoporotic population.

腹腔性疾病血清筛选为阳性者中，有12位(4.5%)为骨质疏松者，另有6位(1.0%)为非骨质疏松者；除了其中的两位，其他的阳性反应患者皆进行了小肠的切片检查；结果显示，骨质疏松者中有9位为确定腹腔疾病病例，非骨质疏松者中则有1位；经过切片检查的确认，骨质疏松者的腹腔疾病流行度为3.4%，非骨质疏松者则为0.2%。

Ordinary no destruction , the ossicular chain can be erosions and destruction , but more slight and no transitional .the major CT findings in simple chronic middle ear on 2 patients were soft tissue in middle ear cavity ,and 1 patient findings hydropneumo, the bone of tympanic cavity cirrhosis ,no destruction .

其它征象包括乙状窦、鼓室盖、水平半规管、面神经管水平段骨质破坏。29例肉芽肿型中耳炎CT表现为中耳腔内的软组织密度影，部分有液平，鼓室壁增生、硬化，骨质破坏少见，听小骨可出现吸收破坏，但程度较轻，一般无移位。2例单纯型中耳炎表现为中耳腔内的软组织密度影，1例有液平，鼓室壁骨质硬化，无骨质破坏。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。