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Methods The promoter of hTERT and Cox-2 was subcloned from human genomic DNA, then ligated to shuttle vector pd306 which contains the Ela and Elb gene of adenovirus to control the expression of Ela and Elb,respectively.

将hTERT和Cox-2启动子从人类白细胞基因组中亚克隆出来,并将启动子分别插入到腺病毒穿梭载体pd306上的E1A和E1B基因前,使hTERT和Cox-2启动子分别调控腺病毒必须基因E1A和E1B的表达,再将构建后的pd306和腺病毒的骨架质粒BHGE3在Ad293细胞内进行同源重组,并用重组后的病毒感染Hela细胞检测病毒对肿瘤细胞的杀伤力。

Our practice is to remoe the endosteal side of the graft for two reasons: first, the thin endosteal portion proides a graft to be placed across from the cortical graft; second, the endosteal surface, being rather rough and irregular, should be remoed to ensure good contact of the graft with the host bone.

由于两个原因我们常规切除骨块的内骨膜面部分:其一是较窄的内骨膜面部分可以用作植骨架于皮质骨连接骨块;其二是由于内骨膜面租糙不规整,切除后能使植骨块与受区骨更好地接触。

Subsequently,enol methylation of P in basic condition completed the synthesis of malyngamide O.Applied this route to the synthesis of malyngamides Q and R,the chiral unsaturated acid was connected with the vinyl chloride part by amidation,then the resulting product was converted into the corresponding aldehyde,followed by aldol condensation of the aldehyde with the chlorotitanium enolate of pyrrolidone to complete the skeleton of malyngamides Q and R,then the synthesis of them was completed via enol methylation in acid condition.

该合成路线拓展应用到malyngamides Q和R的合成时,首先用酰胺键将手性不饱和脂肪酸和烯氯片段连接,再转化成相应的醛,与乙酰基吡咯酮进行TiCl_4催化的aldol反应即得到malyngamides Q和R的骨架结构,再经过酸催化的烯醇甲基化反应完成了Q和R的全合成。

BACKGROUND: One of the key neuropathological changes in Alzheimer disease is that neurofibrils over phosphorylated cytoskeletal protein composed of entwist together, and the phosphorylation of τ protein can be catalyzed by cyclin dependent kinase 5 (CDK5),however whether the phosphorylation of neurofilaments can be catalyzed by CDK5, as well as its role in the pathogenesis of Alzheimer diseases is less acknowledged.

背景:阿尔茨海默病的主要神经病理学特征之一是由过度磷酸化的细胞骨架蛋白如(o,神经细丝组成的神经原纤维缠结,细胞周期蛋白依赖性蛋白激酶5可催化蛋白的磷酸化,但神经细丝是否可被细胞周期蛋白依赖性蛋白激酶5磷酸化及其在阿尔茨海默病发病中的作用却知之甚少。

This topic investigate the change of epidermic cell in suspension and in simplex after they were preserved in defferent ways. The junction between cells and the adhesion between cells and matrix have important roles in the hypothermic impairment of tissue.Their existence affect the recovery of cells under hypothermia. Consequently,the cytoskeleton system may contribute to the impairment caused by junctions.

本研究利用体外培养的表皮细胞单层和单细胞悬液,采用不同的保存方法进行保存,观察到组织细胞间的连接以及细胞与细胞基质的连接对组织冷冻的损伤有重要的作用,它们的存在直接影响组织细胞的活力,其原因与细胞骨架系统有关。

The extrinsically electromagnetic loss of 3-dimentional structures was proved by the comparative measurement of parameters between 3DRCs and their same composition disks and exhibited high-temperature and wide frequency characteristics, which was found closely related to their nature materials. The extrinsically loss was higher in magnetic 3DRCs than that of nonmagnetic ones.

通过与同质陶瓷压片的电磁参数对比,证实了三维连通网络结构存在非本征电磁损耗,且这种非本征电磁损耗具有耐高温和宽频带特性,损耗的大小与网络骨架的材质有关,磁性网络陶瓷的非本征电磁损耗比非磁性的大。

In the present study, we examined the involvement of MTs in regulation of stomatal function with epidermal peels of Vicia faba L.

虽然人们对气孔运动机理做了大量工作,但细胞骨架在气孔运动中的研究刚刚起步,争论很大。

The faceblank is an important functional assembly in the overall design of the facepiece,which puts other functional assemblies,such as eyepieces,breathing system,voice transmitter and nosecup etc.

罩体是将防毒面具中各功能部件(如眼窗、呼吸气系统、通话器和阻水罩等)组成为一个整体的骨架,是面罩总体设计中的一个重要部件,涉及面罩十多项技术性能指标。

Comparisons of ABFANT and FANT within a given iteration number are performed on the publicly available QAP instances from QAPLIB.

在QAPLIB中的典型实例上的实验结果表明,近似骨架导向的快速蚁群算法明显优于快速蚁群算法。

The THFA yield and selectivity were 2.0%higher than those by using catalyst Ⅰ without promoter,3.0%higher than those by using Raney nickel catalyst under the same reaction conditions and furfuryl alcohol conversion.

在相同反应条件和糠醇转化率条件下,四氢糠醇收率和选择性均比没有助剂的镍催化剂Ⅰ高2.0%,比骨架镍催化剂高3.0%。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。