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According to the bone apposition events at the interface, we measured the osteoblast attachment rate with the hemocytometer, the osteoblast growth curve with modified MTT method, the ALPase activity and protein content of cellular layers with modified kinetic method and modified Coomassie'method respectively, and osteocalain content in the cell medium with RIA , at different levels of cell responses, as osteocompatibility indexs.

根据骨内种植体界面的骨愈合过程,分别从成骨细胞反应的不同层次选择了细胞贴壁率、细胞生长曲线、细胞层ALPase活性和蛋白质含量以及细胞培养液中骨钙素的含量作为体外评价材料骨性生物相容性的指标。采用细胞计数板计数法测定细胞贴壁率,采用改良MTT法测定细胞生长曲线。

Results: Compared test group with control group at 14th day of experiment, test group rats were osteoporotic. The lower bone density figures of test group showed that bones were osteoporotic and test group bone HE microtome section showed that bone trabecula was looser, os integumentale was thiner and chondrocyte was decreased.

结果:实验第14天,模型组与对照组相比,模型组大鼠骨质疏松模型建立成功:骨密度不同程度的降低、骨HE切片中骨小梁稀松、皮质骨变薄、软骨细胞层减少等病理变化。

Abstract] objective to improve the diagnosis of non-ossifying fiboma by analysing its x-ray feature.methods plain x-ray films was performed in all patients.x-ray finding in 8 cases with pathologically-confirmed non-ossifying were analyzed.results the clinical symptom was mild,the lesions occurred usually at the metaphysis of the long bones,the affected bones included femur(n=4),tibia(n=3),humerus(n=1).cortical type was seen in 5 cases,presenting as unilocular or multilacular transparent areas with in the cortex or tightly beneath the cortex,the lesion had a sclerotic margin.which was more obvious at the marrow side.medullary type was seen in 2 cases.the lesion was located at the center of the bone and grew centrally.the tumor was manifested as unilocular or multilocular.tramsparent area with sclerotic border and the bone cortex became thinner with slightly expanding on 11 sides.conclusion plain radiography is the elementally means to detect this disease.based on the typical x-ray signs of non-ossifying fibroma combined with clinical data,correct preoperative diagnosis can be made in most cases.

目的 探讨非骨化性纤维瘤的x线表现以提高诊断水平。方法所有病例均摄有x线平片,分析经病理证实的8例非骨化性纤维瘤。结果临床症状轻,好发于下肢长骨干骺端或骨干,股骨4例,胫骨3例,肱骨1例。皮质型5例,表现为皮质内或紧贴皮质下的单层或多层透亮区,病变向骨内发展进入髓腔,周围有致密硬化带环绕,以髓腔侧明显;髓质型2例,病灶在骨内中央发展,显示为单房或多房透亮区,边缘有硬化,骨皮质菲薄,轻微向周围膨隆。结论 x线为最基本的检查方法,典型病例x线平片结合临床症状即可明确诊断。

Results: 1 Intense ERα immunoreactivity localized in the hypertrophic and maturative layer, and some immunoreactivity was found in osteoblasts of mandible and temporal bone, while no immunoreactivity was found in the disc and synovium. 2 The expression of ERα in male rats TMJ cartilage was higher than that in female ones. 3 In the condyle cartilage of male rats, the density of ERα positive cells decreased with age, which kept stable after 16 weeks of age. 4 In the condyle cartilage of male rats, the density of ERα positive cells was stable from birth to 8 weeks, but decreased from 16 weeks to 12 months, while the density in 18-month group recovered to the level of 8 weeks. Conclusion: TMJ is one of target organs of estrogen, and ERα plays an important role in the effect of estrogen on TMJ.

结果:1TMJ中ERα主要在髁突软骨的肥大层和过渡层表达,下颌骨和颞骨的骨细胞也有少量表达,而关节盘、滑膜中几乎无表达;2雄性SD大鼠髁突软骨中ERα的表达明显强于同龄的雌性大鼠;3在雄性大鼠TMJ髁突软骨中阳性细胞密度随年龄增长逐渐降低,16周以后逐渐维持在相对稳定的水平;4雌性大鼠从出生后到8周龄阳性细胞密度基本稳定,在16周龄至12月龄年龄组阳性细胞密度显著降低,而在18月龄后又恢复到8周龄以前的水平。

The same size transport bone disc was created mesially by osteotomy on left side and corticotomy on right side. TiNi-SMA distractor was implanted to repair defect.

左侧骨传送盘行颊舌侧全层截骨术,右侧骨传送盘行保留部分松质骨的皮质切开术,置入镍钛记忆合金牵张器。

Methods 128 cases of long bone fracture were randomly divided into the contrast group of 72 cases treated with the routine open reduction and internal fixation and the experimental group of 56 cases treated with transplantation of periosteal slices of cortical bone at the place of fracture.

方法将 12 8例须手术治疗的四肢长管状骨骨折病人随机分为两组。对照组:5 6例,进行常规切开复位骨折内固定。治疗组:72例,在常规切开复位内固定的同时,于骨折处用锐利的骨刀凿取带骨膜薄层皮质骨植于骨折处。结果治疗组临床愈合时间平均为(12 。7± 2 。4 1)周,对照组平均(2 0 。3± 4 。6 1)周,二者相比有显著性差异(P 。0 5 )。

In the period of 8 to 16 weeks after operation,new bone was formed continuously, artificial bone gradually reconstructed to mature lamellar bone,bone trabeculae and medullary cavity,etc. Conclusion The preparation of CPC is simple.

结果 CPC植入后4周有新生软骨形成及未成熟的骨组织,可见大量的软骨细胞、成骨细胞、胶原组织及较多的小血管。8~16周新骨继续生成,人工骨逐渐改建为成熟的板层骨、骨小梁和髓腔结构。

Results There existed new cartilage and new immature bone tissue in the bone substitute in 4 weeks after operation, as well as mass of cartilageous cells,osteoblasts, collagen tissue and more small vessels were seen. In the period of 8 to 16 weeks after operation,new bone was formed continuously, artificial bone gradually reconstructed to mature lamellar bone,bone trabeculae and medullary cavity,etc.

结果 CPC植入后4周有新生软骨形成及未成熟的骨组织,可见大量的软骨细胞、成骨细胞、胶原组织及较多的小血管。8~16周新骨继续生成,人工骨逐渐改建为成熟的板层骨、骨小梁和髓腔结构。

Materials and Methods: Spiral CT scan of the middle ear was performed with 1.0mm slice thickness at pitch 1.0, bone algorithm, and 0.1~0.2mm reconstruction interval in 20 normal subjects(40 ears).

材料和方法:20例无中耳疾病者采用层厚1.0mm、螺距1.0轴位薄层扫描,骨算法、0.1mm~0.2mm间隔重建,分别做CTVE成像、表面和骨最大密度投影3D重建,观察和比较CTVE和3D显示正常听骨链的能力。

METHODS Using heparin sepharose Cl-6B chromatography,the osteoinduction protein in reconstituted bone xenograftwas pu-rified and fractions obtained from purification were bioassayed for bone-induction activity by implantation into the thigh muscles of mouse.

采用肝素亲和层析技术纯化RBX中诱骨活性蛋白即骨形态发生蛋白,利用小鼠肌袋实验检测其骨诱导活性,再用聚丙烯酰胺电泳测量RBX中诱骨活性蛋白纯化后的相对分子质量。

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