验证试验

A simulation model of the OBS edge node is established, on which the correctness of analysis is testified.

同时利用Opnet建立了OBS网络边缘结点的仿真模型，并通过仿真试验验证了理论分析的正确性。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

In order to investigate the seismic behavior of hybrid structures and guide design of high-rise buildings, the research projects on shake table tests of global structure and pseudo static tests of relevant member were generalized which were conducted in State Key Laboratory for Disaster Reduction in Civil Engineering of Tongji University in recent 5 years.

混合结构试验研究一般针对具体工程，研究佐证不充分且缺乏共性，为掌握混合结构抗震性能，指导高层结构设计，结合同济大学土木工程防灾国家重点实验室近5年来开展的混合结构及其受力构件研究，总结出地震力作用下混合结构受力特点及破坏规律，验证三水准、两阶段设防目标下结构的可靠性。

The pull-off test was conducted to evaluate the bonding states between new and old coneretes.

运用拉拔强度评价新旧混凝土界面结合状态，并借助显微硬度仪测量界面处的显微硬度值对试验结果进行了验证和分析。

This study testified the advantages of produce micron wood fiber such as high fiber production, low pollution and less water usage by mechanical method, drawing up a new scientific method of forming micron wood fiber at cell splliting level.

给出了理想的试验热压曲线，分析了实验过程应该注意的问题，验证了机械法制微米级木纤维得率最高、污染最小和用水量最少的优点，从细胞劈裂水平上提出了新的形成微米木纤维的科学方法。

Thirdly, combined with the theory research above, the author made a quantitative research and design for all the parts of inclined wire and tested the inclined wire former by the using of our company's test paper machine, which proved the author's design conception and design quantitative standards were correct.

第三，结合上面的理论研究，对斜网成形器各部件进行了定量研究和设计，并利用我公司试验纸机对斜网成形器的实验，验证本文的设计理念和设计定量等基本正确。

Next, author has proved it through ten samples for experiment, and then, we get the clear and definite understanding of failure mechanism of normal section, the failure state and the work behaviors of every component (flat steel wire web, inclined steel wire, concrete coat, longitudinal bar), established the formulas of the load-bearing capacity of the normal cross-section.

本章首先对3D板的正截面抗弯承载力进行了理论分析，并通过六个试件做了试验验证，明确了3D板正截面破坏机理、破坏状态以及3D板各组成构件（钢丝平网、斜丝、混凝土面层、纵向受拉钢筋）的受力性能，建立了承重型3D板正截面承载力计算公式及适用条件。

In the mean time the extracting methods of range and angle information are discussed. The method is tested and verified by simulation.

同时论述了宽带信号距离信息和角度信息提取方法，利用仿真和模拟试验验证了方法的正确性。

The sudden stiffness change on the top of tube towers of ship lifts caused the intensive seismic whipping effect to the top machinery building. To suppress the whipping effect, a roof MRD isolation system were presented.

升船机结构塔柱平台处的刚度突变会引起升船机顶部厂房强烈的地震鞭梢效应，在升船机结构模型上设置屋盖磁流变阻尼器MRD隔震系统并进行振动台试验，以验证该系统对控制升船机结构顶部厂房地震鞭梢效应的有效性。

The wave-making resistances of the ship moving in deep water were calculated out, and the results are closed to the data published in the literatures, which test the reliability of the program.

在深水条件下，计算了在航道中航行的船舶的兴波阻力，将结果与他人公开发表的计算和试验数据相比较，吻合程度较好，验证了本程序的可靠性。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。