验证试验

By pool examination arid outfield examination, the system has the definite imaging effect and the high reliability.

经过多次水池试验及外场试验的验证，系统具有较好的成像效果及较高的可靠性。

This thesis proposes a stochastic analysis method of risk assessment for breakwater wave overtopping and stability by physical experiments and calculated results by design codes.

由于奥帆工程的安全问题关系到奥帆赛成功举办和国际声誉，因此其水工结构经过了有关单位的设计并通过了大量的物理模型试验和数值模拟验证，但无论是"设计规范"还是物模试验都没有考虑到复杂多变的海洋环境的不确定性及其对工程设计的影响可能会导致工程在使用的过程中出现意外，因此必须对工程的设计进行系统的风险分析。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Finally, through paving testing road, the rationality and availability of the results of the experimental study was approved.

最后通过试验路的铺筑，对室内试验的结果的合理性和有效性进行了验证。

The new method is given to design the optimum profile modification curve design The effects of optimum profile gear in improving the dynamic performance of gear transmission are verified in the Gear Dynamic Performance Test Rig In this dissertation, the main study contents and creative results are shown as following: The influences on the gear transmission system caused by the gear's accumulated error (periodic error and harmonic error) are theoretically studied. A new method is developed to simulate the gear's harmonic error by using the 2-order MARKOV process A simulated model of the dynamic performance which is excited by the gear's accumulated error is obtained.

本文在前人大量研究的基础上，结合作者承担的国家自然科学基金项目"齿轮传动动态系统辨识与三维修形研究"和山东省自然科学基金项目"齿轮传动动态性能仿真及结构参数、齿形的优化修形"，采用理论和试验相结合的新思路，系统地研究了齿轮传动的动态性能，建立了能正确描述齿轮传动系统动态性能的仿真模型和系统辨识模型，给出了确定齿轮传动轮齿修形曲线的优化设计方法，利用自行研制的齿轮传动动态性能试验装置，验证了优化修形齿轮对提高齿轮传动动态性能的突出作用。

Combining with the National Natural Science Foundation ofChina(No.50478032), following researches were done in this thesis: 1 The variation regularity of the ultimate anti-bending capacity of therectangular-sectioned beam with different corrosion has been obtained through thequalitative analysis of 48 beams (32 corroded beams and 12 comparing beams) withaccelerated corrosion; 2 The conclusion has been obtained that the strain of corroded steel bar andconcrete no longer fit in with the assumption of plane section through the analysis ofthe strain data, the strain relation of steel bar and concrete in the mid-span section ofvariously corroded beams at ultimate has been obtained. Through the building of newgeometric relation, the expression of the height of compressive area in the corrodedbeam with rectangular section has been deduced, and the formula with corrosion rateas the main variable for the anti-bending capacity of the corroded concrete beam hasbeen suggested and verified by experiment.; 3 The influence of steel bar corrosion and the concrete regression to the flexureductility coefficient of the corroded structure component has been analyzed. Based onthe relation of steel bar and concrete at yield and ultimate, the expression for theflexure at yield and ultimate of corroded reinforced concrete beam, and the model forthe calculation of the flexure ductility has been built.

本文结合国家自然科学基金项目(50478032)"既有钢筋混凝土桥梁时变可靠度研究"，主要进行了以下研究工作： 1通过对48根快速锈蚀钢筋混凝土矩形截面梁(32根锈蚀梁和12根对比试验梁)数据的定性分析，得到了不同锈蚀率下，锈蚀钢筋混凝土矩形截面梁极限抗弯承载力的变化规律； 2通过对应变数据的分析，论证了锈蚀构件钢筋和混凝土的应变不再符合平截面假定的结论，并通过回归分析得出了不同锈蚀率的螺纹钢筋梁和光圆钢筋梁在极限弯矩作用下，跨中截面处钢筋应变和钢筋处混凝土应变的关系，通过构造新的几何关系，得出了锈蚀钢筋混凝土矩形截面梁计算受压区高度的表达式，既而得出了以锈蚀率为主要变量的锈蚀钢筋混凝土矩形截面梁的正截面抗弯计算建议公式，并通过试验数据加以验证； 3分析了钢筋锈蚀和混凝土劣化对锈蚀构件曲率延性系数的影响，结合钢筋屈服和极限弯矩两种状态下钢筋和混凝土的应变关系，分别得到了锈蚀钢筋混凝土矩形截面梁屈服曲率和极限曲率的计算表达式；既而推导出曲率延性系数的计算模型。

In this paper,traditional H-bridge circuit for Electric Power Steering System was introduced and analysed.

对智能功率开关的功能特性和采用智能功率开关组成的H桥电路的控制方法与工作原理进行了系统研究，并在试验台上对新的H桥驱动电路进行了试验验证。

Motor controller and the technique of controlling the propulsor are discussed in this paper.A sample of IMP has been made and tested under water in order to verify the feasibility of the new type propulsor system.

另外，对加工出的集成电机推进器原理样机进行了地面试验和水下试验，由此验证了这种推进系统的可行性，并为以后更加深入的研究打下了基础。

To test the theoretical model, air heating and pulverized coal ignition experiments were done.

通过大量的空气加热试验和煤粉气流著火试验，对模型进行了实验验证。

With Puna Chicory as raw material, the research was made on the conversion rate of inulin fructose influenced by hydrolysis time, hydrolysis temperature and the concn. of sulfuric acid through the test of single factor, and the optimal technology parameters for inulin hydrolysis were determined by orthogonal experiment integrating with variance analysis and their results were verified.

方法］以普那菊苣为材料，单因素试验研究水解时间、水解温度、硫酸浓度对菊粉果糖转化率的影响，正交试验结合方差分析确定菊粉水解的最优工艺参数，并进行验证。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。