验证

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

This paper using the FEM software Marc, for the 50 and 60 degree V groove of flat plate butt joint simulate the one-layer and three layers welding, finish each welding and cooling it down to room-temperature then start the next welding true represent process and get the temperature field, residual deformation and welding residual stress, according to the theory analysis and empirical equation verified the temperature field and residual deformation all correct, comparison with experiment verified the welding residual stress regularity and correctly.

本文利用有限元软件Marc，对开60°和50°V型坡口的平板对接接头，动态的模拟了单层单道打底焊，焊完一道后让试件冷却到室温以及单层单道满焊，每焊完一道后让试件冷却，然后再焊下一焊道，道间温度≤200℃，在以此为初始边界条件下开始下一道的焊接，真实的再现了焊接过程，得出了温度场，残余变形和残余应力场的分布规律，根据理论分析及经验公式验证了温度场以及残余变形的正确性，角变形的大小，这些结果与数值计算相吻合。通过与实验测得的残余应力进行比较，验证了焊接残余应力的分布规律及模拟的正确性。

Creating a date field with calendar pop-up should not require multiple tags to create an input field, popup button, calendar extender, masked edit extender, validator, call-out validator,...

创建具有日历弹出一个日期字段不应需要多个标签来创建一个输入栏，弹出按钮，日历扩展，蒙面编辑扩展，验证器和呼叫进行验证，。。。

SCVPN instance-name hardware binding check user user-name host host-id failed.

信息含义： SCVPN对运行 SCVPN 客户端的主机进行验证，并显示验证失败的客户端用户和主机信息。

SCVPN instance-name hardware binding check user user-name host host-id successful.

信息含义： SCVPN对运行 SCVPN 客户端的主机进行验证，并显示验证成功的客户端用户和主机信息。

And the output of the sensor is less dependent on the flow regime. 7. The helical phase concentration sensor designed has been used in the coal puffing system of the 3# blast furnace in Bao Gang to measure in-situ the coal concentration of the main pipe. Combined with the capacitance velocity sensor, the instantaneous mass flowrate of the pneumatic conveying coal is obtained.

在实验室条件下对螺旋形状表面板电容式相浓度传感器的理论优化结构进行了实验验证，对边保护极板提高检测场中心区域灵敏度的作用也进行了实验验证，静态实验结果与理论计算结果相符，传感器检测场的均匀性误差为4.18%，输出线性度好，对流型变化不敏感。

Through experimental study of typical crown fire using pine tree, we first measuredits heat release rate and the inter-space thermal radiation variation of crown fire ofsingle pine tree and compared the experimental data with the model prediction,validating the rationality of the thermal radiation model of single tree.

通过对典型树冠火热辐射的实验研究，以雪松为实验树，测量了实验树的热释放速率、由单颗雪松产生的树冠火的空间热辐射随时间的变化，并将测量值与利用单树热辐射模型计算出的理论值进行了对比分析，验证了单树热辐射模型的合理性；然后测量了由多个雪松同时燃烧产生的空间热辐射随时间的变化，并将测量值与利用多树热辐射模型计算出的理论值进行了对比分析，验证了多树热辐射模型的合理性。

After entrance onto China, validation Certificate of Health Examination Record of Foreigners to local hygienic and quarantine department.

入境后，凭外国专家《外国人体格检查记录》到当地卫生检疫部门换取《外国人体格检查记录验证证明》，各地公安机关凭《验证证明》和《外国专家证》发给外国人居留证。

After a muliobjective searching by AT-0520 D electronic computer using orthogonal regression design, the optimum technological conditions including the principal factors which influence the chemical reactions have been found. The expected yield of butyl acrylate, the content of byproduct β ester and acid value are all identical with the experimental data.

对影响反应的主要因素，采用正交回归设计和用AT-0520D电子计算机对多目标进行搜索，得出优化工艺条件组合，经实验验证，丁酯收率、付产β酯含量、酸值的予测值都和实验值吻合，并经生产规模验证，得到满意结果。

Critical equipment like air conditioning system, water system, autoclaves, filling lines, lyophilizers, thermostatic rooms and filters are initially validated prior to use and re-validated periodically.

空气净化系统、水系统、灭菌柜、灌装线、冻干机、房间的温度调节系统、过滤器等关键设备在使用前需要经过验证和定期的再验证。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。