马罗

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

The Russian Federation national autonomous prefectures and autonomous zone Autonomous and self-map area population (one million square serial zone million, capital km 190 1-1 Jewish Autonomous 3.6023. 20 to Robbie Zhan 1-2 Nenets autonomous post-secondary 17. 677.50 District naryan mar 1-33. 2925.20 Komi - Perm green card Er Yake Kurdish autonomous zone 1-4 Khanty - Mansi from 52. 31,130.50 Hunter - Man Xisi Governance Area g 1 -575. 0329.30 Yamal - Nie Nie pod out Haerdeci self-government in autonomous prefectures and autonomous area map area population (one million square serial zone million, capital km 190 1-6 Temel self-designed 86. 217.20 dudinka District 1-7 Avon-based self-government tertiary 76.762. 40 Fig tension zone 1-8 Ust - Orr 2. 2418.90 Darboux Li Yate from Ust - Austria Er-Governance Center Deng Siji 1-91. 909.50 Aga - Buliyaa Jin Sike that special autonomy zone 1-10 Chukotka autonomous professionals 73. 7720.20 Anadyr District 1-11 Coria grams of self-government 30. 154.80 Palana Russia, a vast area across most parts of Eastern Europe and Northern Asia.

俄罗斯联邦民族自治州和自治专区图中自治州及自治面积（人口（序号专区名万平方万人，首府公里）190）1—1犹太自治州3.6023.20比罗比詹1—2涅涅茨自治专17.677.50区纳里扬马尔1—33.2925.20科米—彼尔米库德绿卡尔亚克自治专区1—4汉特—曼西自52.31130.50汉特—曼西斯治专区克1—575.0329.30亚马尔—涅涅蒴列哈尔德茨自治专区图中自治州及自治面积（人口（序号专区名万平方万人，首府公里）190）1—6泰梅尔自治专86.217.20杜金卡区1—7埃文基自治专76.762.40图拉区1—8乌斯季—奥尔2.2418.90达布里亚特自乌斯季—奥尔治专区登斯基1—91.909.50阿加—布里亚阿金斯科那特自治专区1—10楚科奇自治专73.7720.20阿纳德尔区1—11科里亚克自治30.154.80帕拉纳专区俄罗斯幅员辽阔，横跨东欧和北亚大部分地区。

Near the entrance to Chaunskaya Guba they met the steamer Kolyma, Captain Troyan, who was just returning from the Kolyma, the first steamer ever to visit that river.

附近，恰翁古坝入口处，他们会见了蒸笼科雷马，船长特罗恩，谁是刚刚从科雷马，有史以来第一次轮船返回访问该河流。

The games offered at DoylesRoom are Texas Hold'em, Omaha, Omaha Hi-Lo, Seven-Card Stud, Five-Card Draw and Five-Card Stud.

游戏提供doylesroom是德州扑克，奥马哈，奥马哈喜罗，七卡种， 5 -卡抽奖及5卡种。

On her computer screen, Robyn Matteson, Mate's graduate student, monitored the echo sounder and the concentrations of krill it detected at the dome.

马特带的研究生罗宾·马特森在她的电脑屏幕上监控着回声探测器，以及在圆突区探测到的磷虾群。

Vered Gallery - Art advisor, consultant and dealer offers services as:* photographic art consultant * photography dealer * photography curator Modernism, milton avery, Willem de Kooning, Jackson Pollock, Childe Hassam, Thomas Moran, Chagall, Picasso, Matisse.

4~5画廊-艺术顾问、顾问及经销商提供服务为：摄影艺术摄影顾问***经销商馆长现代摄影、米尔顿艾维利，kooning次Willem德、鳕杰克逊、少爷hassam托马斯默然，罗丹，夏卡尔、毕加索、马蒂斯。

Researchers, Anavilhanas Reserve, Brazil, 1982Researchers from the National Institute for Amazonian Studies use nets to collect specimens of pacu, a fish that eats fruits and nuts, on the Rio Negro in Amazonas state, Brazil.

亚马逊国家研究所的研究人员用渔网捕捉pacu作为样品。pacu是一种生活在巴西亚马逊地区一个叫里奥内格罗的地方的鱼，主要以果实、坚果等为食。

Marco Polo visited India in the thirteenth century, and was followed 200 years later by a Portuguese navigator, Vasco da Gama.

马可波罗在13世纪到访印度，200年后葡萄牙航海家瓦斯科·达·伽马也到访了印度。

Among the many naturalists, artists, and poets of the order may be mentioned: Thomas of Celano (d. about 1255), author of the "Dies Irae"; Giacomino of Verona (c. 1300), a precursor of Dante; St. Bonaventure (d 1274); Jacopone of Todi (d. 1306), author of the "Stabat Mater"; John Brugman (d. 1473); Gregor Martic (d. 1905); the Croatian poet.

在许多大自然，艺术家和诗人的命令可提及：托马斯切拉诺（草约1255年），作者的&模具Irae &； Giacomino维罗纳（角1300 ），前体的但丁；街文德（四1274 ）； Jacopone的陶迪（草1306年），作者的&圣母材料&；约翰布鲁格曼（草1473 ）；格雷戈尔马尔蒂奇（草1905年）；克罗地亚诗人。

South by 400 miles, in Mato Grosso state, the Amazon meets the agricultural frontier.

再向南400英里，在马托-格罗索州[4]，亚马逊接着与农业前线相遇了。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。