香草酸

Methods The effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 7-nitroindazole (7-NI) on nitric oxide synthase activity, levels of dopamine、3,4-dihydroxyphenylacetic acid、homovanillic acid, and TH-immunostained postive nerve fibres in the striatum of mice were determined by using colorimetic analysis, HPLC with electrochemical detection, and immunohistochemical analysis.

用比色分析、高效液相色谱电化学及免疫组化法检测1-甲基-4-苯基-四氢吡啶和7-硝基吲唑（7-NI）对C57BL小鼠纹状体一氧化氮合酶活性，多巴胺、二羟基苯乙酸、高香草酸水平和酪氨酸羟化酶免疫阳性神经纤维的影响。

In the pretreatment group, Bak Foong Pill extracts were given for 14 daya prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相－电化学方法检测纹状体多巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

Hours later,these mice were decapitated,and the striatums were isolated from thebrains. The levels of dopamine, serotonin ( 5-HT ), homovanillic acid, 5-hydroxyindoleacetic acid ( 5-HIAA ) andnorepinephrinein the striatum were measured by high-performance liquid chromatography with electrochemical detection.

给3组小鼠分别喂食Dieldrin 50 mg·kg-1体重、Dieldrin 40 mg·kg-1体重和等量的生理盐水，3 h后处死小鼠，取纹状体匀浆用高效液相色谱-电化学检测法测定纹状体中多巴胺、5-羟色胺(5-HT)、高香草酸、5-羟基吲哚乙酸(5-HIAA)和去甲肾上腺素的含量。

Immunohistochemistry technology was used to evaluated tyrosine hydroxylase of DA synthesis. DA, 5-hydroxytryptophan(5-HT) and their respective metabolic products 3,4 -dihydroxyphenylacetic, homovanillic are also precisely quantified in this research by using high-pressure liquid chromatography with electrochemical detector. The main metabolism monoamine oxidase of DA was processed too. RESULTS:WD can increase the synthesis and the metabolism process of DA .

本文观察了温胆汤及其配伍对大鼠刻板行为的影响，采用了免疫组化技术对多巴胺的合成酶—酪氨酸羟化酶进行了含量测定，应用高效液相-电化学法测定了DA、5-羟色胺（5-HT）及代谢产物3，4—二羟基苯乙酸、高香草酸的含量，并测定了DA主要降解酶—单胺氧化酶。

Xingpikaiyu Formula ; depression model ; neurotransmitter ; 5_hydroxytryptamine ; homovanillic acid ; dopamine

醒脾开郁方；抑郁模型；神经递质； 5-羟色胺；高香草酸；多巴胺

The content of ferulaic acid is least, its range is 0.496～12.566μg·g~(-1), the content in old-growth Chinese fir soil is more than 5μg·g~(-1), and others are less than 5μg·g~(-1). The content of three phenylic acids in 0~20cm depth soil is larger than 20~40cm. Along the ecosystem conversion direction, in 0~20cm and 20~40cm depth soil, the laws of P-hydroxybenzoic acid, ferulic acid and vanillic acid content are complex, does not exist apparent refularity.

其中对羟基苯甲酸含量最大，大小范围为2.233～19.589μg·g~(-1)，其中只有二代杉木人工林的20～40cm层土壤的酚含量小于5，其他的都大于5；香草酸次之，大小范围为0.912～5.683μg·g~(-1)，其中只有天然更新常绿阔叶林的酚含量大于5，其他的都小于5；阿魏酸含量最小，大小范围为0.496～12.566μg·g~(-1)，其中老龄杉木林的0～20cm和20～40cm层土壤的酚含量大于5，其他的都小于5。

Except vanillic acid,there were no direct relationshipsbetween phenolic acids in roots and root exudates of soybean.

除了香草酸外，大豆根中的酚酸与分泌物中的酚酸无直接关系。

However, vanillic acid, a major metabolic product of vanillin in human and rat, was not active in these in vitro activity assays.

不过，香草酸，主要代谢产物香草人和鼠、没有这些活跃在体外活性测定。

Result The main components of aqueous infusion of pepper leaf were vanillic acid (75.7 μg/g) and umbelliferon (29.8 μg/g).

结果］辣椒叶水浸液的主要成分为香草酸(75.7μg/g)和伞花内脂(29.8μg/g)。

Among them, p-coumaric acid, cis-aconitic acid, vanillic acid, citric acid were well known antiinflammation active components.

已知对羟基桂皮酸、顺式乌头酸、对羟基苯甲酸、香草酸、柠檬酸具有抗炎活性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。