首先

The constructed pcDNA3-IL-15 plasmid was identified by Bgl Ⅱ digestion,PCR amplification and sequenceanalysis respectively,to ensure that the hIL-15 cDNA was properly inserted into thevector and the sequence was correct.Then the pcDNA3-IL-15 plasmid was instantlyexpressed in COS-7 cells by means of lipofectamine transfection,and followed by astable expression in Chinese hamster oval cells.8 positive cell colonies wereobtained after the G418 selection,designated CHOA~H cells.

经过BglⅡ酶解分析，PCR扩增鉴定和DNA测序分析，证明IL-15 cDNA已正确插入克隆载体且序列正确后，采用脂质体法，首先将pcDNA3-IL-15重组质粒转染至非洲绿猴肾细胞（COS-7）做瞬时表达，再转染至中国仓鼠卵巢细胞进行稳定表达。

First of all, if we would like to retrieve all the children of a node we can use the asterisk operator.

首先，如果我们要检索的节点，我们可以使用星号经营者所有的孩子。

Then, taking the wave problems governed by the Helmholtz equation as an example, several conventional infinite elements such as the Bettess element, the Astley element and the Burnett element are compared and reviewed.

首先介绍无限单元的概念和无限元方法的特点；围绕求解以Helmholtz方程控制的波动问题，评述几种常规无限单元的优劣，这些单元包括Bettess元、Astley元和Burnett元。

But first, I also had the rare opportunity to meet with one of the most powerful men on earth, Wen Jiabao, the prime minister of China. I met with him in his suite at the Waldorf Astoria Hotel in New York.

但是首先我要介绍的是，我也有了一个很难得的机会，访问世界的强人之一，中国的国务院总理温家宝，这次访谈在他居住的纽约阿道夫酒店的套房进行。

First of all, we need to understand what the word Astrology means and where it comes from.

首先，我们需要了解什么词占星术手段，并且它来自的地方。

Artificial grass playing fields first become common in the 1970s with the introduction of the now-iconic AstroTurf (both fields found to contain lead were produced by the AstroTurf company and were nylon variants, according to CNN).

Dr.eye：人造草室外运动场首先在20世纪70年代变得普通有现在 iconic AstroTurf(如果并且尼龙是变型，根据 CNN ，两个发现包含指示的领域被 AstroTurf 公司产生)的介绍。

Firstly, we hypothesize that the stocks of funds with net outflow will have a higher herding measure when the fund manager is concerned with his reputation risk (Scharfstein and Stein, 1990), and have a lower herding measure when he engages in gaming the incentive scheme derived from the asymmetric performance-flow relation. The results support the reputation-risk hypothesis.

首先，针对前期流量与基金群集指标的关系，本研究推论：如果经理人行为符合声誉风险假说(Scharfstein and Stein， 1990)，则前期净流出基金的群集指标会高於前期净流入基金的群集指标；反之，如果经理人行为符合放手一博假说，则净流出基金的群集指标低於净流入基金的群集指标，实证结果符合声誉风险假说。

Firstly, we study the optimal investigation strategies of the insurance company under complete asymmetrical information conditions.

首先，本文研究了信息完全不对称条件下保险公司的最优调查战略。

First, from now on asymmetry between supply and demand begins to slacken, this is a good thing.

首先，从现在起，不对称供应和需求之间开始松懈，这是一件好事。

In this thesis, CAD formulas are obtained by synthetic asymptote for three structures, First, we obtain the simple CAD formulas of a microstrip line on two-layer substrate with loss

采用"合成渐近"法，本文首先推导出两层有耗介质上的微带线单位长度电容的CAD公式，从而可以计算出微带线特征阻抗和传播常数

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。