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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

In the re-pressed tests with jacked pipe piles in soft clay, it was observed that the re-pressed starting pressure can reach to more than 2.5 times the final pressure for 25 days after finishing the installation; and it increased more rapidly in early period than later.

对饱和软黏土地基中的静压管桩进行隔时复压试验发现，复压起动压桩力在沉桩25d后的增长幅度达2.5倍以上，且前期增长较快而后期较慢。

In this thesis, we developed a new method to construct 2, 3, 6-trisubstituted piperidines using enantiopure -amino acid derivatives as the starting material and applied them to the synthesis of some piperidine alkaloids which showed interesting physiological activities.

本论文中，我们从非手性原料出发，通过手性胺对、(-不饱和酯的Michael加成来获得光学纯的-氨基酸酯，发展了一种构建2、3、6-三取代哌啶环的方法，并利用这种方法合成了一些具有重要生理活性的生物碱类天然产物。

Some new α,β-unsaturated sulfones were prepared by condensation of 4-nitrophenylsulfonylacetates with aromatic aldehydes in cyclohexane/CH_3CH_2OH in the presence of catalytic amounts of piperidine.

不饱和砜是以对硝基苯磺酰基乙酸酯为原料与芳香醛在六氢吡啶醋酸盐催化下发生缩合反应制得的。

Refined snake venom is first produced with snake venom of Pallas pit viper through twice chromatographic separation, saturated ammonium sulfate precipitation, centrifugal separation, dialysis and freezing drying and then produced into the said medicine through compounding, filteration, disinfection and packing.

一种治疗肝炎病的药物疏肝酶，是由复蛇蛇毒经两次层析分离，饱和硫酸铵沉淀，离心、透析、冷冻干燥而得精制蛇毒，然后再经配制、过滤、除菌灌注、封口而成。

To research the water retention performance of lowland heightening farmland protection project in Tingzikou Water Control Pivot, large-scale soil column experiments and one-dimensional saturated-unsaturated flow simulations have been performed.

针对亭子口水利枢纽农田防护工程低地垫高方案的保水性能，在室内大型土柱试验的基础上，建立基于Richards方程的一维饱和-非饱和水流运动数学模型，模拟分析不同防护方案条件下土壤水分的运动过程和保水性能。

The working electrode was prepared by way of depositing TiO2 thin film on conductive ITO glass substrate via sol-gel method. As 3-electrode testing system was then formed combining it with SCE as reference one, black platinum electrode as auxiliary one and PC+LiClO4 solution as electrolyte.

采用溶胶－凝胶法在ITO导电玻璃基片表面制备一层TiO2薄膜，将其作为工作电极，与作为参比电极的饱和甘汞电极、作为辅助电极的铂黑电极、作为电解质溶液的碳酸丙烯酯＋高氯酸锂一起构成三电极测试体系。

She was of medium height, had a very pretty face with short hair, but looked a little podgy under her clothes, in fact she was a fatty.

她中等身高，有一个很漂亮的脸，蓄短发，但似乎很少podgy下，她的衣服，事实上，她是一位不饱和脂肪酸。

Utilizing the polar angle transform, the expressions of the displacements, stresses and pore pressures of saturated soil and those of the liner in the mapping plane can be obtained.

在此基础上，将原像平面中任意形状的衬砌内外边界处的边界条件代入到映像平面中圆环势函数的关系式中，从而求出饱和土中衬砌介质在弹性稳态体波作用下产生的动态响应。

The temperature effect on the oil yield was discussed at first. Then, analytical work was carried out as follows: the ultimate analysis and physi-chemical analysis of the pyrolytic oil comprised a sketch of the fuel properties of the oil; Then, through the distillation analysis of the oil, the fraction composition of the "unrefined"oil was known. After that, a general knowledge of the aromaticity and saturation of the hydrocarbons in the oil was provided by the outcome of the FT-IR functional group analysis. Also, from the analysis, the form of the polar functional groups and their distribution in different fractions of the oil were learned.

文中首先对不同热解温度下热解油产率进行分析讨论，然后，针对热解油品质进行了一系列分析：通过元素组成和理化特性的分析，对热解油作为燃料特性有了一个大致了解；通过实沸点蒸馏分析，得到热解油作为一种未提炼油的馏分分布，并为后续热解油馏分的细入分析提供了前提；通过对热解全油和各窄馏分的FT-IR官能团分析，对热解油的芳香性、不饱和性有了整体的了解，并且清楚了热解油中的极性官能团存在型式及其在热解油不同馏分中的分布。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。