饥饿的

Actually, the development of genetically-modified products is a double-edged sword: On the one hand, it contributes to the enhancement of the world crop yield, as well as the quality, and reduction of chemical fertilizer and the pesticide use, helps solution of the famine and undernourishment in developing countries. Besides, it also promotes the development of certain related industries like pharmaceutical and chemical industries; On the other hand, it also brings the unprecedented threat for human beings. We are unaware of the potential effects in human bodies, or any unknown injury to the next generation, caused by genetic modification. Nor can we forecast what kind of influence to the extant gene will emerge.

而事实上，转基因生物技术的确是一把双刃剑：一方面，转基因技术可以提高世界农作物产量、提高质量、减少化学肥料和杀虫剂的使用、解决发展中国家的粮食缺乏、饥饿和营养不良的问题，另外，基因技术的飞速发展在很大程度上也促进了医药、化工等相关行业的发展；另一方面，它也为整个人类带来了前所未有的威胁，人们无法得知基因序列的改变会在体内引起何种潜在的变化，无法确定转基因产品是否会对下一代产生任何未知的伤害，无法预测转基因产品对现存基因会产生何种影响。

Actually, the development of genetically-modified products is adouble-edged sword: On the one hand, it contributes to theenhancement of the world crop yield, as well as the quality, andreduction of chemical fertilizer and the pesticide use, helpssolution of the famine and undernourishment in developingcountries. Besides, it also promotes the development of certainrelated industries like pharmaceutical and chemical industries; Onthe other hand, it also brings the unprecedented threat for humanbeings. We are unaware of the potential effects in human bodies, orany unknown injury to the next generation, caused by geneticmodification. Nor can we forecast what kind of influence to theextant gene will emerge.

而事实上，转基因生物技术的确是一把双刃剑：一方面，转基因技术可以提高世界农作物产量、提高质量、减少化学肥料和杀虫剂的使用、解决发展中国家的粮食缺乏、饥饿和营养不良的问题，另外，基因技术的飞速发展在很大程度上也促进了医药、化工等相关行业的发展；另一方面，它也为整个人类带来了前所未有的威胁，人们无法得知基因序列的改变会在体内引起何种潜在的变化，无法确定转基因产品是否会对下一代产生任何未知的伤害，无法预测转基因产品对现存基因会产生何种影响。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体，以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板，参照已发表的微小牛蜱Bm86基因的核苷酸序列，设计了1对引物，采用RT-PCR技术获得微小牛蜱Bm86基因；将Bm86基因克隆入载体，并进行序列分析，结果证明，克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%，而且该序列包含完整的开放阅读框。

The perplexing fact is that by making some simple calculations with the available data, one is struck by the recognition, that already in the immediate future (2015) the percentage divisions between the renewable and the non-renewable sources will have to be inverted, simply to avoid that half of the world's population dies of hunger. The studies regarding the peaking of the world oil supplies and world uranium supplies has irrefutably confirmed the above stated prediction.

目前的复杂状况是，根据现有数据做出的一些简单结论中有一点是公认的，即在不久的将来（2015年），可循环能源和不可循环能源的利用比例将完全颠倒，这也只是为了避免世界上半数人口死于饥饿，而关于世界石油供给和铀供给的距增的机关研究也绝对地证实了上述预言。

Echoes of cries of pain rever berate in my heart. Children in famine, victims tortured by oppressors, helpless old people a hated burden to their sons, and the whole world of loneliness, poverty, and pain make a mockery of what human life should be. I long to alleviate the evil, but I can't, and I too suffer.This has been my life.

那些回响在我内心的痛苦的呼唤经常在我内心回旋，那些饥饿中的孩子，成为苦难的牺牲品，还有不愿变成子女重担的孤苦无依的老人，以及全世界无情的孤独，贫穷和痛苦的存在，是对人类生活理想的真正讽刺！

Last year、Taiwan formerly joined in international government organization、APEC. The circumstance in APEC was disappointed、and Taiwan was absent from the non-government meetings. However、Taiwanese are very optimistic in improving their international status in this digital global era. This is because the international society still needs democratic freedom and economic strength to serve all the members of the world for a better and undeficient life.

去年我国参加正式的国际政府组织APEC表现令人失望，非政府领袖也缺席，引起了国内各界深入的讨论，不过大家对资讯化、全球化、全球治理的时代下，参与国际非政府组织，走入国际社会，提升我国国际地位与形象还是很乐观的，因为国际社会仍然需要有讲求民主自由、经贸实力的国家民间社团，凝聚共识，来为地球村的每一角落民众服务，希望他们在后冷战时期下，免於疾病、饥饿等威胁，寻求更好的安全生活。

Newspaper men have killed his parents in various ways; it is said that the paternal Chaplin died of natural causes and his widow (known to the boards as Lily Harley) went into dressmaking, taught Charles and his brother Sydney to hem flounces; there is still another affecting scene in which Chaplin, a sallow waif in bloomers, is portrayed leading his starved mother to a poorhouse while London gamins revile him for his kindliness.

关于他父母的死因报纸上众说纷纭：卓别林老爹据说是自然死亡，他的遗孀（据说叫Lily Harley）后来改行做其了女装裁缝，教卓别林和他的哥哥Sydney缝衣服上的荷叶边；在卓别林的童年中有很感人的另一画面——有人描述卓别林当时一个穿着灯笼裤、一脸菜色的流浪儿，领着他饱受饥饿煎熬的母亲来到一所破房子前，而伦敦的其它流浪儿因为他的善良却欺侮他。

No hungry generations tread thee down; The voice I hear this passing night was heard In ancient days by emperor and clown: Perhaps the selfsame song that found a path Through the sad heart of Ruth, when, sick for home, She stood in tears amid the alien corn; The same that oft-times hath Charmed magic casements, opening on the foam Of perilous seas, in faery lands forlorn.

一代一代，饥饿无法剥夺你的生命；这逝去的夜里我欣赏的歌喉，或许，拾穗度日的路得被这歌喉打动，站在异乡的田野悲痛不禁，思乡的泪水满脸纵横；常常，魔法禁锢的古堡被这啭唱感动

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Immediately after he was born,hermes was appointed godof thieves at olympus.and a thief he became when he was barely a few hours old.feeling hungry,the infant left his cradle after nightfall to hunt for food.he chanced uponapollo''s oxen grazing on a meadow and drove a number ofthese animals away,tying tree branches around the feet of thecattle to make their footsteps dim.he killed two of these oxenand had a magnificent dinner.then he slipped back into his littlebed in his mother''s house.when apollo came to question himhermes pretended to be innocent.the angry sungod draggedhim up to olympus,where he was accused of the stealing andmade to return the cattle to their master.in reconciliation little hermes gave apollo the lyre he had made out of a tortoise shell,and apollo was so pleased with the gift that he presented his little brother with a magic stick.the stick could pacify hostileforces.

海尔墨斯一生下来就被任命为奥林波斯山的贼神。他出生后几个小时的时候就做了贼。黄昏时分，他感到饥饿，于是还是婴孩的海尔墨斯离开了他的摇篮去找食物吃。他碰巧看见阿波罗的牛在草地里吃草，于是他偷走了许多牛，并将树枝绑在牛脚上，让脚印变得模糊不清。他杀了其中两头牛，美餐了一顿。然后悄然地回到他妈妈的房里，爬上床。当阿波罗发现牛少了，来质问他时，海尔墨斯佯作不知。愤怒不已的太阳神把他拖到奥林波斯山上，告他偷窃并责令将牛如数还给主人。为了平息这件事，小海尔墨斯将他用乌龟壳做的琴送给阿波罗。阿波罗得到这件礼物非常高兴，并送给他的小兄弟一支魔棍，这支魔棍可以化解敌对力量。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。