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Now, one of them is a leaf cutter ant in the Amazon.

一种是亚马逊河的食草蚁

The evolutionary relationships were investgated by comparing the results from molecular data alone or molecular data combined morphological characters using different analysis methods: maximum parsimony by PAUP(superscript *) 4.0 and Bayesian inference by MrBayes 3.0B4. Base composition and ti/tv of 28S rDNA D2 regions were analyzed by PAUP(superscript *) 4.0. The results showed that GC content of the 28S rDNA D2 gene sequences of subfamily Euphorinae ranges from 40.00% to 49.25%. Transversion frequency of sequence variable sites among species of Euphorinae was higher than that of transition. The subfamily Euphorinae was proved to be a paraphyletic group with close relationship to subfamilies Neoneurinae and Cenocoelinae; only two tribes, Meterorini and Microctonini, were proved to be monophyletic groups; Centistini, Cosmophorini, Euphorini and Dinocampini were paraphyletic groups; the basal position of tribe Meterorini within Euphorinae was partially supported by our phylogenetic trees, and tribe Microctonini was proved to be a group at the top of the trees.

结果表明:优茧蜂亚科的28S rDNA D2基因序列片段的GC%含量在40.00%~49.25%之间变动,而对于碱基替代情况来讲,优茧蜂亚科各个成员间序列变异位点上颠换大于转换;不同的分析和算法所产生的系统发育树都表明目前根据形态定义出的优茧蜂亚科Euphorinae不是一个单系群,而是一个与蚁茧蜂亚科Neoneurinae和高腹茧蜂亚科Cenocoelinae混杂在一起的并系群;在优茧蜂亚科内部,悬茧蜂族Meterorini和食甲茧蜂族Microctonini(排除猎户茧蜂属Orionis)为单系群,而宽鞘茧蜂族Centistini、大颚茧蜂族Cosmophorini、优茧蜂族Euphorini、瓢虫茧蜂族Dinocampini为并系群;悬茧蜂族Meterorini在优茧蜂亚科Euphorinae内位于基部位置的观点得到部分的支持,同时食甲茧蜂族Microctonini被判定为相对进化的类群。

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