颗粒细胞

OBJECTIVE: To investigate the effects of graphite carbon nanoparticles on cell growth, cycle, and apoptosis in vitro.

目的：观察石墨碳纳米颗粒对体外培养细胞的生长增殖、细胞周期及凋亡的影响。

Vacuoles and black dense particles usually occurred in cytoplasm of heterokaryon, and occasionally, swelling of mitochondria, pycnotic nuclei or even enucleation process could be seen among hybrid cells.

超微结构分析表明，杂交细胞表面膜微绒毛、指状突及皱褶突等均明显减少，核内异染色质比例增高，核仁数目减少或消失，细胞质中常可见有空泡或黑色致密颗粒出现；少数杂交细胞出现线粒体肿胀、核固缩甚至排核现象。

On the 10th day, took out testis, fixed in the Boom fixtion solution, conventionally produced and section and stain. Finally observed under the optical microscope. Results The cytoplasm of mesenchymal cell and spermatogonium in test group showed hydropic degeneration.

结果 实验组小鼠睾丸组织中间质细胞和精原细胞胞质呈水样变性，出现红染细小的颗粒状物质，颗粒周围有大小不等的淡染区。

The UCN synthesized by interbrain hasimmunosuppressive action. However the UCN synthesized by peripherytissue has proinflammatory function.

另外，肥大细胞可以合成分泌UCN，并且UCN可以诱导肥大细胞脱颗粒产生大量促炎因子。

Results In the subendocardium of the left ventricle,apical part and interventricular septum,the pathologic changes of degeneration and necrosis were found out in some bigger size of point or rope type separately,in which the filtration of lymphocyte and mononuclear cells were seen in great quantities.The myocardial cells presented granular denaturation and myofibril lysis.

结果 实验组大鼠心肌在左室壁内膜下、心尖、室中隔部位出现明显坏死等改变，病变呈灶状或条索状分布，涉及范围较大，有淋巴细胞和单核细胞浸润，心肌细胞呈颗粒变性，肌原纤维凝聚、崩解，部分肌纤维溶解。

The results showed that,on first day induced by mIL-2+B16,mIL-2+B16PELs had no cytotoxicity to B16 target cells,no expression ofperforin and granzymeA genes,no granules in PELs cytoplasm.

结果显示：1.mIL-2〓细胞诱导一天时，取得的PEL用Rt-PCR检测，无粒酶A与穿孔素基因表达、无细胞毒活性、其胞浆中也无杀伤颗粒存在。

Cells were perforated, granzyme B will be able to enter and destroy infected cells.

细胞被穿孔了，颗粒酶B就能够进入并破坏受感染的细胞。

METHODS:Guinea-pigs were sensitized by ovalbumin and the effects of LMWH on the constriction of ileum smooth muscle induced by OA were observed in vitro;The effects of LMWH on the degranulation of pericranium mast cell induced by OA were observed throu gh the passive anaphylaxis ratmodel which was made by vaccinating antiserum at pericranium;Licking response was induced by 4-aminopyridine(4-AP) scat scruff in mice,then the effect of LMWH on licking response was studied the capillary p emeabilty increasing induced by glactal acetic acid and the effect of LMWH on ca pillary pemeability were studied.

用卵白蛋白(ovalbumin，OA)免疫豚鼠造成过敏模型，观察LMWH对OA所致的豚鼠离体回肠平滑肌收缩的影响；在大鼠颅骨骨膜接种抗血清使肥大细胞被动致敏，观察LMWH对OA引起肥大细胞脱颗粒的影响；以4-氨基吡啶诱发小鼠舔体反应，观察LMWH对舔体反应的影响；以冰醋酸使小鼠毛细血管通透性增加，观察LMWH对通透性的影响。

METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上，剪开大鼠颅骨，显露位于颅腔前方的嗅球，取出2只嗅球，在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织，保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层，剪成1 mm3小块分离获取单细胞悬浮液，调整细胞密度至1×107 L-1，接种在用poly-l-lysine包被的培养瓶或培养板中，于37 ℃、体积分数为5%的CO2培养箱中培养，第3天用无血清DMEM/F12培养基换液培养。

RESULTS : When ferumoxides-TA or MION-46L TA was used, intracytoplasmic particles stained with Prussian blue stain were detected for all cell lines with a labeling efficiency of nearly 100%.

结果：使用ferumoxides-TA或MION-46L-TA标记细胞时，所有细胞的胞浆内均可检测到普鲁士蓝染色颗粒，其标记的效率接近100%。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。