颗粒细胞

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

The results indicated that in the FNAC smears of mediastinal tumor, neutropilic myelocyte, neutropilic metamyelocyte, neutropilic stab granulocyte and neutropilic segmented granulocyte were observed in granulocytic series; early normoblast, polychromatic normoblast and orthochromatic normoblast were observed in erythrocytic series; and granular megakaryocyte, thromocytogenic megakaryocyte, scattered platelet and matured lymphocyte also were observed. Cell morphology and distribution in different stages were are similar to that in bone marrow smear, and these cells belong to the extra-medullary hemopoietic tissue.

结果表明：纵隔肿块的细针抽吸活组织的细胞学涂片显示粒系细胞中可见中性中幼粒细胞、中性晚幼粒细胞、中性杆状粒细胞、中性分叶核粒细胞；红系细胞中可见早幼红细胞、中幼红细胞、晚幼红细胞；并可见颗粒巨核细胞、产血小板的巨核细胞、散在血小板及成熟淋巴细胞；细胞形态及各期细胞分布与骨髓片相似，为髓外造血组织。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下：细胞形态不规则，与正常子宫内膜组织DC相比，胞膜较光滑，胞膜表面树突状胞浆突起显著减少，部分突起呈粗短状；胞质中线粒体相对少，圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见；高尔基体、粗面内质网、核糖体丰富；含微量絮状电子致密物的胞饮小泡显著减少；胞质中可见形态特殊的颗粒，该颗粒外周膜包裹，略呈圆形，中间部位稍弯曲，如耳机状，颗粒中由高电子致密物居于一侧，而另一侧则呈透亮状；胞核显著减小，居于胞质一侧，常呈肾形或马蹄形，核内常染色质较少，异染色质多边集于核膜下。

Virus removal efficiency is determined by the real time PCR and inactivation efficiency is determined by cell-based infectivity assay.Enveloped DNA virus removal and inactivation: Real time PCR indicated that method based on gold magnetic particles coupled with antiserum has shown great superiority on virus removal. Cell-based infectivity assay indicate pasteurism has shown superiority on virus inactivation.

对两种指示病毒分别进行巴氏灭毒和偶联抗血清金磁颗粒处理，实时荧光定量PCR检测核酸变化确定病毒的去除效率，同时进行病毒的细胞感染力实验确定病毒的灭活效率，结果显示：在对DNA有包膜病毒的去除能力上，金磁颗粒法明显优于普通的巴氏灭活法；在对DNA有包膜病毒灭活能力上，巴氏法明显优于金磁颗粒法。

Mol·L~(-1) individually and also with both heavy metals present. Plasmolysis, concentrated cytoplasm, disappear ance of the cristae of mitochondria, ambiguity of framework and destruction of mitochondria envelope were all symptoms of root cells under Cu stress; hollowing cells was also found in Cd treatment, and there existed some different granule in these hollowing cells.

铜使根细胞产生质壁分离、细胞质浓缩、部分细胞空泡化，使线粒体脊突消失、结构模糊、外膜破坏；镉使根细胞空泡化，并在部分空泡化的细胞里产生大小不等的颗粒状物；铜、镉交互污染使根细胞受害程度加深，并兼有两者的受害症状特征：线粒体结构彻底破坏、空泡化细胞里的颗粒物更大电子密度更高、质壁分离现象更普遍、质膜上的颗粒物沉淀更大。

In hypothalamic median eminance of kidney Yang deficiency group, the bounds of ependymocytes were dim, distribution of their microvillus changed, the top of microvillus enlarged and became spherical, and holes occurred on the surface of the cells. In that of control group, the cell arranged in order and the cell bouns were clear, the microvillus were equal in thickness and in long, thin acerose thread wool shape. The surface ultrastructure of ependymocytes on hypothalamic median eminance in herb group was similar to that of control group.

肾阳虚大鼠垂体内分泌细胞和甲状腺细胞存在着不同程度的粗面内质网、高尔基体扩张，线粒体空化，细胞变形，核固缩等超微结构的损伤；中药组大鼠垂体超微结构与正常组类似，细胞形态完整，结构清晰，细胞器结构正常，含有各种大小不等、形态不一的分泌颗粒，但甲状腺细胞超微结构与肾阳虚组相比未见明显改善。

Objective: To observe the influence of You Tang Ming granule, which had the functions of replenishing qi and producting jing, nourishing liver and kindneys, activating channels of eyes to improve eyesight, on retinal microvascular and retinal gangliocyte in STZ-diabetic rats.

中文题名优糖明颗粒对糖尿病视网膜病变非增殖期作用的实验研究副题名外文题名论文作者刘路宏导师廖品正教授学科专业中医眼科学研究领域\研究方向学位级别博士学位授予单位成都中医药大学学位授予日期2001 论文页码总数55页关键词优糖明颗粒链脲佐菌素糖尿病视网膜病变视网膜疾病馆藏号BSLW /2001 /R276 /3 目的：探讨益气生津、滋养肝肾、通络明目中药复方优糖明颗粒对6个月病程STZ糖尿病大鼠视网膜微血管及神经节细胞等的影响及其机理。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

Under inverted microscope, stereognosis of neurons died down, there were blank bulb in the neurons, dark particle were seen in the neuritis; the result of MTT test showed that the survival rate of neurons was obviously decreased. Res 40μmol/L increased the survival rate of neurons. Aβ(subscript 25~35) dose-dependently increased the level of MDA, decreased the level of GSH. Res dose-dependently increased the level of GSH, decreased the level of MDA.

结果：Aβ(下标 25~35)在终浓度为2μmol/L时神经元形态有明显的变化，细胞立体感减弱，胞体内空泡形成，细胞突起内可见黑色颗粒状物；细胞的存活能力明显下降，与对照组相比较有显著性差异(P.05)；Res在终浓度40μmol/L时，能明显提高细胞的存活能力。Aβ(下标 25~35)可以明显升高细胞培养上清液中MDA含量，降低GSH的含量；Res可使细胞培养上清液中MDA含量下降，GSH含量增加。

RESULTS: In the retinal region without light, the structure of every layer was clear, cells in neuroepithelial layer arrayed in rule, some bubble presented in external granular layer and internal granular layer, RPE cells were compact, and the color of pigment article was coincident. In the region with direct blue light and that with 30J/cm2+Acrysof one-piece/ pMMlA, cells on photoreceptor and external granular layer were lost partially, bubble increased, RPE cells were with different sizes, and cell edema, cell lost and pigment article cluster could be seen.. In region with 30J/cm2+Natural, a little disorganization could be seen comparing to that without light, but more normal than those with Acrysof and direct eradia tion.

结果：未照射部位：视网膜各层次结构清晰，神经上皮层内各细胞排列规则，整齐，RPE细胞排列紧密均匀，色素颜色一致；单独光照和透过Acrysof一片式I0L：光感受器层部分细胞丢失，外核层可见固缩核，浓密，内颗粒层细胞大量丢失，内丛状层内空泡明显增多，RPE表面，细胞大小不均，可见肿胀、缺失，细胞内色素浓密、聚集成块；透过Nat-ural一片：与未照射处相比，视网膜各层次结构仍有部分破坏，但程度明显减轻。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新