颗粒细胞

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Fig 4 Few of rough endoplasmic reticula, mitochondrid and co llagen can be seen in the cytoplasm of fibroblast, the ribosome of rER is degran ulated, the nucleous is abnormal, there are a lot of granule (maybe the antisens e oligodeoxynucleotide) in the cytoplasm TEM×5200

图4反义核酸组的瘢痕成纤维细胞粗面内质网较少、有脱颗粒现象，线粒体少，胞核畸形，胞内有吞噬颗粒，细胞周围胶原纤维较少TEM×5200

The results showed that some holes on the nuclear membrane of the two kinds of the cells besides normal cell structures and there are some secretive pellets of protein with more high density of electronics around the nuclear of the cells, these pellets maybe is the regulatory matter of living things growth and around o'clock section rule, It is important to sturdy the ultrastructure of brain tissues and the physiological function of Tupaia belangeri chinensis .

利用电镜观察了树鼠句Tupaia belangeri chinensis的脑垂体和松果体细胞的超微结构，结果显示这两类细胞除了有正常细胞结构外，核膜孔较大且在核周围还有一些电子密度不同，大小不一的蛋白质分泌颗粒，这些颗粒可能是生物个体调节各种生理生化功能、生长发育和昼夜节律的信使物质，这对进一步研究树鼠句脑组织结构及其生理功能有一定意义

During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of testis also increase in quantity, but it is less than that in ovary, they disintegrate after sexual differentiation. The big grana always exsist in course of sexual differentiation, but small grana don\'t appear.

分化时精巢细胞的超微结构显示线粒体和内质网数量也有增加，但不如分化中卵巢细胞的数量多，分化后线粒体和内质网解体，在整个分化过程中大颗粒一直存在，无小颗粒出现。

The cells were incubated with Alexa 488 conjugated Staphylococcus aureus bioparticles opsonized with rabbit IgG for 1h, followed by fixation and incubation with Alexa 633 conjugated goat anti-rabbit IgG to distinguish ingested from extracellular bioparticles.

将兔抗IgG 作用于Alexa 488金黄色葡萄球菌的活性颗粒，后与实验细胞(上述4种)孵育1小时，再用Alexa 633羊抗兔IgG定位，以区别活性颗粒是被吞噬的还是位于细胞外的。

The cells were incubated with Alexa 488 conjugated Staphylococcus aureus bioparticles opsonized with rabbit IgG for 1h, followed by fixation and incubation with Alexa 633 conjugated goat anti-rabbit IgG to distinguish ingested from extracellular bioparticles.

将兔抗IgG 作用于Alexa 488金黄色葡萄球菌的活性颗粒，后与NTM细胞孵育1小时，再用Alexa 633羊抗兔IgG定位，以区别所吞噬的位于细胞外的活性颗粒。

Under optical microscope, deep blue masses in a granular appearance were observed in the secondary xylem parenchyma cells of the dormancy season barks after stained with mercury-bromophenol blue. However, there was no granular protein in the development season barks.

经汞－溴酚蓝染色后的石蜡切片显微观察表明，休眠期枝条细胞的液泡中存在大量蓝色近椭圆状蛋白颗粒，而在生长期枝条细胞的液泡中则未发现这种蛋白颗粒的存在。

Under optical microscope,deep blue masses in a granular appearance were observed in the secondary xylem parenchyma cells of the dormancy season barks after stained with mercury-bromophenol blue.

经汞-溴酚蓝染色后的石蜡切片显微观察表明，休眠期枝条细胞的液泡中存在大量蓝色近椭圆状蛋白颗粒，而在生长期枝条细胞的液泡中则未发现这种蛋白颗粒的存在。

Under optical microscope,deep blue masses in a granular appearance were observed in the secondary xylem parenchyma cells of the dormancy season barks after stained with mercury-bromophenol blue.However,there was no granular protein in the development season barks.

经汞—溴酚蓝染色后的石蜡切片显微观察表明：休眠期枝条细胞的液泡中有大量蓝色小球状蛋白颗粒，有的甚至充满整个液泡，而在生长期枝条细胞中则未发现这种蛋白颗粒的存在。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新