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In winter,nuclear of GTH cells were elliptical and a small number of secretory granules scattered around nucleolus. Number of secretory granules in STH cells were relatively small, nucleolus distorted, activity of synthesis and secretion decreased. Secretory granules in TSH cells cytoplast were relatively small.

冬季,GTH细胞核为椭圆形,有少量的分泌颗粒散布在细胞核的周围;STH细胞分泌颗粒数量较少,胞核变形,合成和分泌活动比较微弱;TSH细胞胞质中的分泌颗粒相当多。

In winter,nuclear of GTH cells were elliptical and a small number o f secretory granules scattered around nucleolus. Number of secretory granules in STH cells were relatively small, nucleolus distorted, activity of synthesis and secretion decreased. Secretory granules in TSH cells cytoplast were relatively small.

冬季,GTH细胞核为椭圆形,有少量的分泌颗粒散布在细胞核的周围;STH细胞分泌颗粒数量较少,胞核变形,合成和分泌活动比较微弱;TSH细胞胞质中的分泌颗粒相当多。

Calcium chloride modified nanoparticles prepared by means of chemistry. Analyse the combination of calcium phosphate nanoparticles and DNA, the protection to DNA as well by gelose gelatin electrophoresis. When the green fluorescence protein gene was regarded as report gene, gene vector of calcium phosphate nanoparticles transfected CNE-2 cell in vitro and vivo. Combine the calcium phosphate nanopartides and suicide gene yCDg1yTK for Nasopharyngeal Carcinoma therapy in vitro.

化学方法制备,氯化钙修饰纳米颗粒,用琼脂糖凝胶电泳分析磷酸钙纳米颗粒与DNA的结合效率及对DNA的保护作用,用绿色荧光蛋白基因作报告基因,将磷酸钙纳米颗粒为基因载体转染鼻咽癌(CNE-2)细胞和在动物体内转染肿瘤细胞;以及将磷酸钙纳米与自杀基因yCDglyTK结合,并在体外对鼻咽癌进行基因治疗。

(1) In our research,frequent expression of BSAP in H/RS cell of classical Hodgkins disease provides further evidence for its B-cell origin,and the expression of BSAP on H/RS cells also can be in favor of the identification of H/RS cells and can distinguish HL from ALCL.(2) Expression of BSAP is located in nucleoli of cells,while that of CD20 in membrane.

结果 (1)33例cHL中,30/33(90.91%)H/RS细胞和33/33(100%)背景淋巴细胞BSAP表达阳性,而H/RS细胞10/33(30.30%)CD20阳性,1/33(3.03%)CD45RO阳性,31/33(93.94%)CD30阳性,25/33(75.75%)CD15阳性;(2)33例HL石蜡刮片组织中有16例(48.48%)出现IgH基因重排阳性,显微切割的19管H/RS细胞有14管(73.68%)扩增阳性,而12管背景淋巴细胞有2管(16.67%)扩增阳性;(3)测序分析周新华第一军医大学病理学教研室获得了一段免疫球蛋白重链可变区的重排片断;(4)原位PCR结果发现杂交的阳性颗粒不仅出现在探针来源的同一病例也出现在不同病例的HillS细胞核上,同时在部分周围背景淋巴细胞核上也见到阳性颗粒。

Antibacterial function of body wall was conducted by the following way: microorganism was directly resisted through the change of cuticle, such as incrassation of up-cuticle protuberance and increasing length of micro-floccus in the up-cuticle dissociation surface; exudation function of large granular orthochromatic mucous cells and reticulation mucous cells in the epidermis could be enhanced when the Eisenia fetida was induced by E. coli. Moreover, the mucus that contained compound substance of mucopolysaccharide and protein could make the bactericidal function or dilute the microorganism on the surface of earthworm, which could protect earthworm from invasion of microorganism; the content of neutral mucopolysaccharide of antibacterial function could be increased according with the exudation increment of small granular proteinaceous cells in the epidermis, which could resist bacteria invading into the epidermis of earthworm.

结果表明:蚯蚓的体壁主要是通过以下途径来抗细菌的侵袭:一是通过角质层的变化(如上胶层突起的增厚、上角层游离面中微绒毛的变长等)直接抵抗微生物的入侵;二是增加表皮中大颗粒粘液细胞和网状细胞的分泌功能,分泌出大量含有粘多糖蛋白液体复合物的粘液,在蚯蚓体表对细菌进行稀释或者起到直接杀菌的作用;三是通过增加小颗粒蛋白细胞的分泌作用,在蚯蚓的表皮中分泌出更多的抵抗疾病的中性粘多糖,从而抵抗进入蚯蚓体表内的细菌。

Results: The proportional volume, numerical density of the granules in ovariotomy group were less than those in sham-ovariotomy and ovariotomy estradiol groups (P.001); the proportional volume and numerical density of ASG in myocardial cells almost came back to the normal level with small dose estradiol; along with the enhancement of estradiol dosage, the content of ASG in myocardial cells was more than those in sham-ovariotomy and small dose estradiol group (P.01), and showed a quantity-efficiency relation.

结果:切除大鼠双侧卵巢后,心房特殊颗粒的体密度和数密度与对照组及用雌二醇组比较明显减少(P<0.01),小剂量雌二醇可使大鼠心肌细胞心房特殊颗粒的体密度和数密度恢复到正常水平,随剂量增加,心肌细胞心房特殊颗粒的量也伴随增加,呈量效关系。

The induced hmscs-tert were examined by oil red-o staining, alkaline phosphates staining and von kossa staining. results the hmsc-tert was negative for cd34, cd45, cd44, cd106 and cd166. after the induction, accumulated cytoplasmatic red lipid droplets were clearly visible in the adipose cells by oil red o staining, and alkaline phosphates-positive pitchy grains formed in the intracytoplasm and calcium nodes were found by von kossa staining in the osteoblasts.

结果 hmsc-tert细胞表面抗原检测cd34、cd45、cd44、cd106、cd166均为阴性;脂肪细胞定向诱导后油红o染色见胞浆内有红色脂肪颗粒,成骨细胞定向诱导后见胞浆内有碱性磷酸酶染色阳性黑褐色颗粒,von kossa染色见有钙结节形成。

The decrease of granule Ca content occurred earlier than the increase of epinephrine concentration, suggesting that the calcium released from chromaffin granule may in part account for the induced cell secretion.

激动剂作用引起的离体肾上腺髓质细胞分泌时颗粒内钙含量的下降早于颗粒数目减少或肾上腺素升高,提示颗粒释放的Ca2+可能是引起细胞分泌的原因之一。

The results are as follows:(1) immunoreactive neurons of NPY in medulla oblongata of Taihe silky fowl, New jianghan domestic chicken are mainly localized in the inferior olivary nucleus.

结果如下:(1)在泰和乌鸡和江汉鸡的延髓中,NPY阳性神经元主要存在于下橄榄核,网状核及中缝核也少量出现;(2)在泰和乌鸡和肉鸽的小脑中,NPY阳性神经元主要存在于小脑皮质的蒲肯野氏细胞层,且以小叶顶端的蒲肯野氏细胞阳性明显,而小脑皮质的分子层、颗粒层未见阳性反应细胞;(3)泰和乌鸡延髓的网状细胞核非常发达;泰和乌鸡小脑皮质分3层,由外至内依次为分子层、蒲肯野氏细胞层和颗粒层;小脑分10叶,Ⅰ、Ⅱ和Ⅹ为单叶,其余各叶都分为2~3个小叶;(4)非洲鸵鸟迷走神经运动背核和疑核特别发达,孤束核吻侧部不发达。

Result: Pai enzymatic active of Gase 1 waiting for 薚 shows brown fine grained, fixed position forms a cell at cutin (enzymatic active of;ACPase of KC) afterbirth film shows black fine grained, the bridge between the cell that basically is located in KC, APLase A enzymatic active shows blue fine grained, be located in KC surface.

结果:①健康人TGase 1酶活性呈棕色细颗粒,定位于角质形成细胞胞膜;ACPase酶活性呈黑色细颗粒,主要位于KC的细胞间桥,APLase A酶活性呈蓝色细颗粒,位于KC表面。

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