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RESULTS : When ferumoxides-TA or MION-46L TA was used, intracytoplasmic particles stained with Prussian blue stain were detected for all cell lines with a labeling efficiency of nearly 100%.

结果:使用ferumoxides-TA或MION-46L-TA标记细胞时,所有细胞的胞浆内均可检测到普鲁士蓝染色颗粒,其标记的效率接近100%。

The effects of pulse parameters such as pulse width Ton, pulse interval Toff, pulse frequency f, pulse current density J on the morphology ,grain size , hardness, internal stress and orientation of coating , and coherence of coating to sucbstrate have been examined .

研究了脉冲电镀参数:脉冲宽度Ton ,脉冲间隔Toff,脉冲频率f,脉冲电流密度 J对镀层微观形貌、颗粒大小、硬度、与基体结合力的影响,分析了镀层内应力、择优取向与脉冲参数的关系。

Electron microscope showed finely granular cytoplasm in group after bFGF stimulation. Most cells lost cytoplasmic organelles. The cells of control group had vast vacuolation of organelles.

电镜下对照组细胞胞浆内有大量肿胀的细胞器,而bFGF组细胞胞浆呈均一细小的颗粒,密度一致,细胞器大部分消失。

M and appearance of many long pseudopodia, have a lot of differentsized basophilic cytoplasmic granules. Some of the cells have both large and small cytoplasmic vacuoles, which can not be stained by neutral red.

m,胞质中具有大小不匀的许多嗜碱性颗粒,有些细胞内也含有不为中性红所着色的较大空泡,细胞表面有许多长伪足伸出。

Results The electron microscopical observation demonstrated that intracellular lanthanum could be found,most of which entered the hepatocytes and its mitochondria. The lanthanum granules were also found to deposite in the zone of tight junctions of bile canaliculi, indicating that the tight junctions had been damaged.

结果 镧示踪标记法显示镧颗粒进入了细胞及线粒体内;毛细胆管紧密连接区也出现了致密的镧沉积,并与肝细胞间隙的镧沉积物相连,表明毛细胆管紧密连接已开放。

After propagation, GTH cells evacuated large number of secretory granules, TSH cells appeared vacuoles, rough endoplasmic reticulum in STH cells were circumnuclear.

繁殖后,GTH细胞中的分泌颗粒出现排空;TSH细胞有空泡;STH细胞中的粗面内质网呈现围核环状排列。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层,采用酶消化法分离细胞,差速贴壁法纯化细胞,接种于多聚赖氨酸包被的培养板内培养2d,采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

An electron microscopic study of resting cysts and their organelles in Paraurostyla weissei has revealed that:⑴The cyst wall is composed of four layers named from outside to inside:ectocyst,mesocyst,endocyst,and granular layer.The granular layer is delimited by the cell pellicle.Both the layer and pellicle contain a few vesicles.

为研究纤毛虫休眠状态下细胞的分化及其胞器的特征,本文以透射电镜术显示,魏氏拟尾柱虫休眠包囊中,颗粒层壁内有小泡,表膜位置也偶见小泡样结构,大部分线粒体以多个相互聚集在一起,自噬泡将线粒体等胞器包裹在内经历消化过程,细胞内膜系统十分发达。

Neurons treated with 20-mmol/L-theophylline did not show 180bp ladder characteristic of endonuclear activation on its DNA gel electrophoresis. The [cAMP]i of the neurons rose after being incubated together with different concentration theophylline (5-80 mmol/L) for 15 minutes in CO2 incubator and the prometed range relied on the dose of theophylline.

100%,量效关系有高度显著性(P.01),EC50=2.97mmol/L;经茶碱(20mmol/L)处理的细胞,其DNA凝胶电泳带没有&梯子&状分布;与不同浓度茶碱(5-80 mmol/L)在CO2培养箱孵育15 min,小脑颗粒神经元内cAMP均升高,且是剂量依赖性的。

The results show as follows:(1) immunoreactive neurons of NPY in pigeon cerebellum tire mainly located in the Purkinje cell layer of the cerebellar cortex, especially in the top of Purkinje cell in cerebellar folia:(2) a few neuropeptide Y immunoreactive neurons are observed in the central nuclei of cerebellar white matter .which is different from silky fowl, chick and rat;(3) none of neuropeptide Y immunoreactive neurons are found both in the molecular and granular layer.

结果显示:(1)小脑内神经肽Y(neuropeptide Y,NPY)阳性神经元主要存在于小脑皮质的蒲肯野氏细胞层,且以小叶顶端的蒲肯野氏细胞阳性明显;(2)小脑白质中央核可见到少量阳性神经元,不同于乌鸡、肉鸡及大鼠;(3)分子层、颗粒层未见阳性反应细胞。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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