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Results showed that β-catenin was positive expression in stellate reticulum,inner dental epithelium,and dental sac at cup stage,in inner dental epithelium,dental papilla cell,and cell nucleus at early bell stage,in cervical loop and cusp cell membrane at late bell stage,and in ameloblast and odontoblast cell at amelogenesis stage.

结果表明,帽状期在星网层、内釉上皮、牙囊均为细胞核阳性表达;钟状早期在内釉上皮、牙乳头细胞核阳性表达;钟状晚期在颈环、牙尖细胞膜阳性表达;成釉期早期在成釉质细胞、成牙本质细胞阳性表达;成釉期晚期均阴性表达。

Among these dogs, viral antigen was demonstrated in the epithelial cellsof the nasal mucosa in 24 of 27 dogs, in the footpad epithelium in 24 of 26 dogs, and in the haired skin of thedorsal neck in 26 of 27 dogs.

在27条狗中,有24只狗的鼻黏膜上皮细胞发现有病毒抗原;有24只足垫上皮细胞中出现病毒抗原;有26条的覆毛颈背皮肤中发现有病毒抗原

To detect effects on apoptosis of cortex neuron in DHCA rats, the rat model of occluding bilateral common carotid artery in the deep hypothermic (21C) rats had been established, and flow cytometry, TUNEL, electron microscope, immunohistochemistry and RT-PCR had been studied.: part I: To establish a new rat model of cerebral ischemia/protect during DHCA: 20 SD rats were used in the experiment, little animal respirator was to assist anesthetized rat's respiration after tracheal cannula, artery press through artery spile in femoral artery, electrocardiograph, temperature, blood saturation of O2 (SaO2) and arterial blood gases were monitored during the whole experiment, blood flow of bilateral common carotid artery of rat were occluded about 60 minute when its temperature was reduced to 21 C by ice, then normal temperature was resumed.

本研究采用大鼠深低温颈总动脉阻断模型,应用流式细胞仪、电镜、原位缺口末端标记法、免疫组化法和RT-PCR技术分别检测深低温脑缺血对皮层神经细胞调亡的影响方法:第一部分深低温停循环脑保护大鼠模型的建立:SD大鼠20只,麻醉后气管插管辅助呼吸,用冰块将大鼠的体温降至(21±1)℃,阻断双侧颈总动脉,记录体温、心率、血压、血氧饱和度和血气分析,60分钟后恢复颈总动脉血流,用Longa等的5级评分标准评定4小时和24小时后大鼠神经系统损伤情况。

Affected anatomic sites included: submaxillary and submentum (7), maxilla(7), palate(6), salivary gland(5),root of tongue(5), neck(4), cheek(2), gingival(1). All of the cases are NHL, B cell lymphoma 27 (72.97%), T/NK cell lymphoma9 (24.32%), one cases cell origin was uncerten.

发病部位依次为颌下、颏下7例、上颌部7例,腭部6例,腮腺5例,舌根部5例,颈上部4例,颊部2例,牙龈1例。37例全部是NHL,B细胞淋巴瘤27例(72.97%),T/NK细胞淋巴瘤9例(24.32%),1例未确定细胞来源。

The sex organs appear about 5 weeks after sowing. The antheridia consist of 3 cells. The archegonial neck usually consists of 4 rows and 4 columns of cells at maturity.

播种5周左右开始有性器官出现,精子器由3细胞组成,成熟颈卵器颈部由4列细胞组成, 4层细胞高。

Plasma Ang Ⅰ and Ang Ⅱ levels, chymase mRNA levels, arteries' PCNA positive percents and the thickness of carotid artery were measured.

通过测定血浆Ang Ⅰ及AngⅡ住水平、糜酶 mRNA表达水平、颈动脉细胞增殖核杭原阳性率及颈动脉各层厚度观察曲尼司特对犬颈动脉损伤后狭窄的作用。

Notch1 is expressed by stem cells resided in the cervical loop epithelium, and Notch signaling pathway play a central role in fate decision of those stem cells. Notch receptors and their ligands are involved in both patterning and cell fate determination during odontogenesis. Moreover, many cell factors, which are closely related with odontogenesis, could regulate expression of Notch receptors or their ligands.

既往研究显示:早在牙齿发育期,Notch1就表达于颈环星网状层的干细胞中,是其保持干细胞性质的重要机制;Notch-Delta信号还与成釉细胞、成牙本质细胞分化有关,被认为是牙齿发育细胞分化多样性的原因之一;另外,目前已明确与牙胚细胞分化有关的生长因子如TGFβ、FGF、BMPs等均可诱导Notch受、配体的表达。

The left external jugular vein of 20 rabbits were harvested and transplanted between ipsolateral common carotid artery. Three days later, animals in group Ⅰ were transplanted with BMMNCs (6×10^8 cells)/100 μl via periotic veins and those in group Ⅱ were injected with 100 μl PBS.

取左颈外静脉4~5cm,上下倒置后移植至颈总动脉,在建模后第3天将100μl DAPI标记的BMMNCs液(6×10^8个细胞)经耳缘静脉注入细胞移植组动物体内,对照组注入等量的PBS液。

The expression of AT2R gene can be regulated efficiently in vivo by local delivery of MSC with dual-stable expression of AT2R gene, thus reduces the formation of intimal hyperplasia.④AT2R gene can influence the expression of ECM and significantly promote the cellular apoptosis in the injury rat carotid artery.⑤These data demonstrate the clinical potential of AT2R regulatable expression to prevent restenosis after PCI.

研究结论:(1)与VSMC直接接触可以诱导MSC向血管成分细胞分化;(2)构建的Dox-on可调控的MSC细胞株诱导活性可靠,使外源AT2R基因的表达处于有效的主动控制下;(3)局部导入MSC不影响球囊损伤后大鼠颈动脉新生内膜增生;(4)由MSC介导的AT2R可调控表达受到Dox的良好调控,可有效抑制球囊损伤后大鼠颈动脉新生内膜增生;(5)AT2R影响血管损伤后修复时ECM成分的表达,并促进细胞凋亡的发生,可能是AT2R介导抑制新生内膜形成的机制。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法:(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC,细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构;(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定;(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC,并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况,根据各个细胞克隆受Dox调控表达的程度,选择低背景、高诱导表达AT2R的细胞系,作为双重稳定MSC细胞系;蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况;(4)建立大鼠颈动脉球囊损伤动物模型,将双重稳定MSC在术中导入血管,分别于14 d、28 d进行病理切片,检测可调控AT2R对新生内膜增生的影响;采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变,TUNEL法检测血管组织中细胞凋亡的变化情况。

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随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。