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预适应

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Objective: To investigate the effect of cardiopulmonary bypass technique on goat ischemic preconditioning model.

目的:探讨体外循环技术对建立山羊心肌缺血预适应模型的影响。

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只,随机分为对照组,缺血再灌注组和缺血预适应组,每组6只,分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平;免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

While the PC by zero-flow global ischemia deceased the amount of LDH in effluxion of the coronary artery and increased VFT during reperfusion.

旷置预适应可使再灌注时的冠脉流出液的乳酸脱氢酶释放量明显减少(P.05),心室纤颤阈显著提高(P.05)。

Combined with animal model of peradaptation to myocardium ischemia , the research observed the level of enzymatic dynamics of myocardium, SOD,MDA,ET and correlation peptide of calcitonin gene in peripheral blood and cardiac function, apoptic cell and expression of apoptosis regulatory gene, ultrastructure of myocardium with BIOPAC16, in situ end labeling and hybridism, optic microscope and electron microscope. The study of animal model of peradaptation to myocardium ischemia illustrated that the peradaptation and pretreatment with Wenxin capsule could be positively protective for the injury of ischemia reperfusion. The protection is mainly reflected by reduction of infarction area, correction of heart rhythm abnormality of ischemia reperfusion, melioration of the contraction and relaxation function of myocardium, recovery of the subcellular structure and themelioration of the abnormality of biochemical and bioactive substance in the myocardium.

并结合心肌缺血预适应动物模型,采用BIOPAC16导生理记录和分析处理系统处理心肌功能指标,采用原位末端标记及原位杂交技术标记凋亡细胞及凋亡相关调控基因的表达,采用光电镜技术观察心肌超微结构的变化,同时观察外周血心肌酶、超氧化物歧化酶、丙二醛、内皮素、降钙素基因相关肽等水平,结果表明心肌缺血预适应及温心胶囊预处理对心肌缺血再灌注损伤具有肯定的保护作用,其保护心脏作用主要表现在缩小心肌梗死范围、抗缺血再灌注心律失常、改善心肌收缩和舒张功能、减轻心肌超微结构损伤,调节心肌生化物质及生物活性物质的生成和释放。

The Protective effect of Wenxin capsule on the injury of ischemia reperfusion is possibly due to the onset of pharmacodic preadaptation through the activation or simulation of the mechanism of ischemia preadaptation. This would be a new field of heart protection.

温心胶囊对缺血再灌注性损伤的保护作用可能是通过激发或模拟缺血预适应机制,而呈现出的药理性预适应对心脏的保护作用,开辟了心脏保护的一个新领域。

Based on the latest achievements of the mechanism of peradaptation to myocardium ischemia and pharmacodic preadaptation up to date, observations were made on 80 patients of acute myocardial infarction to assess the enzymatic dynamics of myocardium, symptoms after myocardial infarction occurred like cardiac rhythm abnormality, anginapectoris, heart failure, cardiogenic shock, reinfarct and the mortality in the hospital. The results indicated that peradaptation to myocardium ischemia is of significance in protecting myocardium, reducing the areas of infarction, abating the cardiac insufficiency after infarction and reducing the abnormality of heart rhythm and significantly ameliorating the prognosis.

本研究结合现代医学对心肌缺血预适应机制及药理性预适应作用的最新研究成果和动向,通过临床观察80例急性心肌梗死患者的心肌酶学变化,心律失常、梗死后心绞痛、心力衰竭、心源性休克、再次梗死等心肌梗死后并发症和住院病死率,证实缺血预适应具有保护心肌,缩小梗死范围,减轻梗死后心功能不全及减少心律失常的作用,可明显改善心肌梗死病人的预后。

The incidence rates and duration of ventricular dilation-induced arrhythmias in each of verapamil, chelerythrine or glibenclamide group were similar to those in control group. Conclusion Ventricular dilation may induce arrhythmias in isolated rat hearts, which is inhibited by ischemic preconditioning. The transient increase in [Ca(superscript 2+)] appeared to be a triggering factor in the process of ischemic preconditioning. K channel PKC might also play an important roles in this process.

对大鼠心室进行膨胀可诱发心律失常;缺血预适应对心律失常的发生具有抑制作用;细胞内钙离子浓度的短暂升高可能是缺血预适应发挥抑制作用的驱动因子;ATP敏感性钾通道及蛋白激酶C在缺血预适应过程中可能发挥了重要作用。

objective to investigate whether hypoxia preconditioning exist in adipose tissue,and construct the hypoxemic model of adipose tissue.methods after injected different density cocl2 to rat's subcutaneously of groin,use immunohistochemical method detect hif-1α protein,and use rt-pcr detect hif-1α mrna.results after injected different density cocl2,the adipose tissue of rat's groin express different degree of hif-1αprotein and hif-1αmrna.,and reach the peak when the density is 20-40 mg/kg and the time is 3 h.conclusion hypoxia preconditioning exist in adipose tissue,and the effect of preconditioning is most evidence when the density is 20-40 mg/kg and the time is 3 h.

目的 探讨脂肪组织中是否存在缺氧预适应现象,构建cocl2致缺氧的缺氧模型。方法不同浓度的cocl2大鼠腹股沟区皮下层浸润注射,分别用免疫组织化学法和rt-pcr法检测该区脂肪hif-1α蛋白和hif-1α mrna的表达情况。结果用不同浓度的cocl2行化学缺氧后在不同时间点观察有不同程度的hif-1α蛋白和hif-1α mrna表达,其中,在浓度为20~40 mg/kg,时间为3 h时脂肪组织中hif-1α表达达高峰。结论脂肪组织存在缺氧预适应现象;cocl2的浓度选择20~40 mg/kg,时间选择3 h时缺氧预适应效果较佳。

We employed an anaesthetised open chest rabbit model of ischemic preconditioning to preliminarily observe the protective electrophysiologic basis, and approached the possible roles on reducing the appearance of lifethreatening arrhythmias.

我们用在体兔心脏缺血预适应模型,初步观察了预适应心脏保护的电生理基础,探讨其在减少恶性心律失常中的可能作用。

AIM: To investigate the effect of noradrenaline on transmembrane potential during delayed preconditioning phase.

目的: 研究微量去甲肾上腺素预处理晚期心肌细胞线粒体跨膜电位的变化,为探讨预适应晚期作用机制提供依据。

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