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Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠,融合筛选抗DR5杂交瘤细胞,制备抗DR5单抗——mDRA6;流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率;荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化;MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响;琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

Volume production of O-doot luxury materials, hollow roller shutter doors wind up its Harmony, Vol aluminum doors, security windows, Frameless glass doors, banisters, all stainless stell products.

临朐东顺门业有限公司是生产澳式卷门、豪华车库门、中空卷帘门、和谐式拉闸、铝材卷门、防盗门窗、无框玻璃门、楼梯扶手、各种不锈铁钢制品、玻璃幕墙的专业化生产厂家;并对外承接钢结构工程。

The series "Shunfa" keyboards are going to be developed as the keyboard for the notebook computers with the specifications of two hand operable large thirty twomo size (total key area length and width of 204 × 112mm ), sixteenmo size and currently used 10-15 inch size, etc,.

将开发"顺亿"键盘系列,用作笔记本电脑键盘,可双手操作的大 32 开本式规格(按键总长宽 204 × 112 mm ),以及 16 开本式、现行的 10~ 15 英寸等各种规格。

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP+Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组,以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组,以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组,作用时间为48小时,以不加药物干预作为对照组,Hoechst33258染色荧光显微镜下观察细胞形态的变化,流式细胞仪检测细胞凋亡峰;紫外光分光光度计检测线粒体PTP开放情况,流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化,Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性,γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

The reaction of cyclic b-keto esters with CF3CO2ZnCH2I provides the corresponding ring-expanded products with the advantage of mild condition in moderate to good yields. A simple and efficient procedure has been developed for a variety of 1,3-diketones converted into the corresponding 1,4-diketones and trans-1,2-disubstituted cyclopropanols in high yields. In conclusion, this new type of organozinc reagents has been developed for many useful reaction in organic synthesis.

利用有机锌试剂所具有的双重特性,在新型的Baylis-Hillman反应中,可以直接与炔基酮和醛反应一锅制得多官能团的三取代的顺式烯;在b-酮酯化合物的扩环反应中,取得了重要进展,得到了增加一个碳的扩环产物,&一步法&合成,反应条件温和、环境友好;在1,3-二酮分子中成功地插入1个碳,合成出1,4-二酮和环丙醇,为现代有机合成提供了新的合成方法,具有良好的应用前景。

Erve growth factor combined with its receptor, activated Ras - MAP kinase , mitogen - activated protein kinase kinase is the important regulating factor that make IkB kinase, IKK, phosphated, IkB kinase make the IκBα:(the subunit of NF -κB ) phosphated, the phosphated IkBα degraded, and p65 - p50 heterodimer can be formed, then the heterodimer translocated to nucleus and combined with the promoter domain or other consensus sequence.

GF与其受体相结合,最后可以激活Ras-有丝分裂激活的蛋白激酶途径,有丝分裂激活的蛋白酶激酶(MEKK1)是IγB激酶发生磷酸化的重要调节因子,IKK使NF-κB的亚单位IKBα发生磷酸化,磷酸化的IKBα发生降解,而最后留下p65-p50二聚体,该二聚体然后转位到细胞核内与κB基因的增强子区域或与他的顺式作用序列结合。

Fault episodic activities controlled the thickness and spatial distribution of each strata, which induced that sedimentary center migrated clockwise with the area near to Wuhaozhuang fault zone as start point. The type of fault activities controlled the overlapping mode of sediments, which could be indicated by that the progradational sequence was developed mainly during the period of extensional fault activities and the retrograding sequence was developed mainly during the period of compressive fault activities.

断层的幕式活动控制了各时期沉积地层的厚度和空间展布形态,使得沉降中心以五号桩长堤断裂带附近为起点进行顺时针迁移;断层活动的性质控制了沉积物内部的叠加方式,即张性断层活动时期以发育退积式准层序组为主,压性断层活动时期以发育进积式准层序组为主。

Since 1985, research on simulation and modeling of concurrent flow, counter flow, cross flow, mixed flow grain dryers had been accomplished.

从1985年至今,顺流式、逆流式、横流式以及混流式谷物干燥机的研究已经取得了成功。

In this study, we have cloned and identified two full-length cDNAs of OsBIMK2 and OsBISERK1, which encoding MAPK and SERK, respectively, and associated with benzothiadiazole induced resistance.

本文克隆和鉴定了水稻中与苯并噻二唑(benzothiadiazole,BTH)诱导抗病性相关的编码MAPK的OsBIMK2基因和编码SERK的OsBISERK1基因全长cDNA;研究了OsBIMK2基因和OsBISERK1基因在水稻—稻瘟病菌亲和与非亲和互作过程中的表达模式;原核表达了OsBIMK2基因,获得重组OsBIMK2蛋白,并研究了重组OsBIMK2蛋白的生化特性;克隆了OsBIMK2基因的启动子序列,并运用农杆菌介导的瞬间表达系统分析鉴定了启动子中参与基因表达调控的可能顺式元件;将水稻OsBIMK2基因导入烟草,获得过量表达OsBIMK2基因的转基因植株,这些转基因植株中防卫反应基因PR-1组成型表达,并提高了抗病性。

The 2×390MW HRSG in Xiamen Eastern Power Plant for combustion engine works adopts the HRSG manufactured by Hangzhou Boiler Group Co., Ltd., which is characterized by three-pressure, reheats, without after-combustion, horizontal type and natural circulation. Since the full set of HRSG designing technology from the US NOOTER/ERIKSEN has been introduced, it matches with PG9341FA type gas turbine, adopting single-row frame construction, full suspension type, and in-line arrangement in the heating surface. The heat conduction components use gear screw finned tube, module type supply and positive pressure operation.

厦门东部燃气电厂2×390MW燃机工程的余热锅炉采用由杭州锅炉厂生产的三压、再热、无补燃、卧式、自然循环燃机余热锅炉,引进美国NOOTER/ERIKSEN公司全套燃气轮机余热锅炉设计技术,它与PG9341FA型燃气轮机相匹配,采用单排框架结构,全悬吊形式,受热面顺列布置,传热元件采用开齿螺旋鳍片管,模块式供货,锅炉为正压运行。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。