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In this study, what had been used inculding CBF1 and CBF3 of CBF family, its downstream COR15a gene , regulating element CRT/DRE in promoter of COR15a gene, Cloning CBF1 and CBF3 from Arabidopsis thaliana, and Cloning ApCOR15a gene from Arabis pumila of xinjiang. On the basis of plasmid pBI121,being used CaMV35S promoter and SAR iductivity promoter synthesized by PCR, six plant expression vectors had been constructed: pCB111, pCB111-1, pCB112,pCB112-1,pCB113, pCB113-1, and then transformed to Agrobacterium GV3101 by electics.

本研究选用了CaMV35S组成型启动子和人工合成的由水杨酸诱导的启动子SAR、拟南芥CBFs家族中的CBF1和CBF3、新疆小拟南芥COR15a基因及其CRT/DRE顺式作用元件构建了不同组合的六个植物表达载体:pCB111、pCB111-1、pCB112、pCB112-1、pCB113和pCB113-1,并采用农杆菌介导的叶盘转化法转化烟草,获得了大量的抗性植株。

Optical control of the primary step of photoisomerization of the retinal molecule in bacteriorhodopsin from the all-trans to the 13-cis state was demonstrated under weak field conditions (where only 1 of 300 retinal molecules absorbs a photon during the excitation cycle) that are relevant to understanding biological processes.

在弱场条件下(整个兴奋周期中,300个视黄醛分子中只有一个吸收光子),细菌视紫红质中视黄醛分子的从全反式到13-顺式状态的光致异构化的最初阶段的光学控制被阐明,这与生物学过程的理解有关。

Peptidyl-prolyl cis/ trans isomerization is of considerable biological significance and prolyl residue plays an important role in the final structure of proteins.

由分辨率。25nm,同一性0%的2401条肽链中计算提取了全部顺式与反式脯氨酸肽键的位置,数目分别为1221个与26401个,从而建立了一个较大规模的脯氨酸肽键数据集。

The number of cis prolyl peptide bonds is 1221 and that of trans prolyl peptide bonds is 26401. Some characters of the dataset were analyzed, including the distribution of the residues of the peptide bonds'N-terminal, the statistic of dihedral angel, the distribution of cis peptide bonds in different secondary structure, the proportion of cis peptide bonds.

由分辨率小于0.25nm,同一性小于30%的2401条肽链中计算提取了全部顺式与反式脯氨酸肽键的位置,数目分别为1221个与26401个,从而建立了一个较大规模的脯氨酸肽键数据集。

This requirement may be satisfied by the submission of information such as the following: name (generic name, chemical name, code number); Chemical Abstracts Service number if available; description (e.g., appearance, color, physical state); molecular formula and molecular weight; structural formula (including ionic state if applicable); stereochemistry identifying chiral centers, cis-trans isomerism, etc.

该要求可以通过提供下述信息来满足:名称(通用名、化学名、编码等)、化学摘要服务编码、性状描述(如:外观、颜色、物理状态)、分子式和分子重量、结构式、立体化学(找出手性中心、顺式反式异性等)、对映结构体比率(如:外消旋物、规定的异构体、对映异构物和固态形式的混合物)、溶解度概况、分配系数、溶液 pH 值、解离常数、熔点或沸点、折射率、比重。

Their chemical structures were elucidated on the basis of physicochemical properties and spectral dataIR,FAB-MS,~(1H-NMR,and ~(13)C-NMR.Result: Six compounds were isolated and identified as: aurantiamide acetate(1),cyclo(2),N-p-coumaroyltyramine(3), N-trans-feruloyltyramine(4),N-cis-feruloyltyramine(5),and nicotinic acid(6),respectively.

结果:分离鉴定了6个生物碱,分别为aurantiamide acetate(1)、环二肽(2)、香豆酰基酪胺(3)、N-反式阿魏酰基酪胺(4)、N-顺式阿魏酰基酪胺(5)、烟酸(6)。

Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium assay. The cell surface markers involved in differentiation of mono cyte/macmphage (CD11b, CD36) were analyzed by flow cytometry.

体外培养人单核细胞系THP-1,经佛波酯诱导分化为巨噬细胞,同时给予9-顺式维甲酸对佛波酯诱导分化进行干预,通过相差显微镜观察细胞形态,MTT比色法检测细胞贴壁率,流式细胞仪检测与单核/巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。

The estimated tunneling probability of the H13 proton from one well to another well shows that the life time of the proton staying in one of these wells is about 6×10^2 a. It is too long to let tautomers of cytosine be in thermodynamical equilibrium in a room temperature gas phase experiment.

估算出的H13质子从一个势阱到另一个势阱的穿透几率表明质子呆在其中一个势阱中的寿命大约是6×10^2 a,这使胞嘧啶和它的顺式亚胺式互变异构体在室温气相实验中远不能达到热平衡。

Enantiomer or solid-state form ratios (e.g., for racemates, and for defined admixtures of isomers or enantiomers or solid-state forms); solubility profile (aqueous and nonaqueous as applicable); partition coefficients; solution pH; dissociation constant; melting or boiling point; refractive index; specific gravity.

该要求可以通过提供下述信息来满足:名称(通用名、化学名、编码等)、化学摘要服务编码、性状描述(如:外观、颜色、物理状态)、分子式和分子重量、结构式、立体化学(找出手性中心、顺式反式异性等)、对映结构体比率(如:外消旋物、规定的异构体、对映异构物和固态形式的混合物)、溶解度概况、分配系数、溶液pH值、解离常数、熔点或沸点、折射率、比重。

Enantiomer or solid-state form ratios (e.g., for racemates, and for defined admixtures of isomers or enantiomers or solid-state forms); solubility profile (aqueous and nonaqueous as applicable); partition coefficients; solution pH; dissociation constant; melting or boiling point; refractie index; specific graity.

该要求可以通过提供下述信息来满足:名称(通用名、化学名、编码等)、化学摘要服务编码、性状描述(如:外观、颜色、物理状态)、分子式和分子重量、结构式、立体化学(找出手性中心、顺式反式异性等)、对映结构体比率(如:外消旋物、规定的异构体、对映异构物和固态形式的混合物)、溶解度概况、分配系数、溶液pH值、解离常数、熔点或沸点、折射率、比重。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。