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Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠,融合筛选抗DR5杂交瘤细胞,制备抗DR5单抗——mDRA6;流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率;荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化;MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响;琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

Objective To evaluate and compare the histamine release and hemodynamic changes caused by cisatracurium and atracurium during induction of general anesthesia.

目的 在全麻诱导期间观察单次静注顺式阿曲库钱后的组胺释放作用及其对血流动力学的影响。

Cisatracurium is one of the stereoisomersof atracurium.

顺式阿曲库铵是阿曲库铵的一个同分异构体。

Methods 30 ASA Ⅰ-Ⅱpatients undergoing elective surgery were randomly divided into 2 groups in which patients received a single bolus of either cisatracurium 0.15 mg/kg or atracurium 0.75 mg/kg.

ASAⅠ~Ⅱ级全麻择期手术患者30例,随机分为顺式阿曲库钱组和阿曲库钱组,两组分别单次静注Cis 0.15mg/kg和Atr 0.75mg/kg。

In this paper, the establishment of a new, rapid method for the specific detection of infectious HAV and the feasibility to exchange HAV IRES and 5 NTR with its counterparts from poliovirus were investigated.

本研究对检测感染性甲肝病毒快速方法的建立和用异源IRES或5'非编码区替换甲肝病毒相应顺式作用元件的可行性进行了探索。

E. . The epoxidation of cis-allylic alcohols is difficult, and with 20%mol Ti 〓 and 30%mol tartrates, up to 80%e.

的对映选择性被得到;对难于环氧化的顺式烯丙基醇,使用20%mol四异丙氧基钛和30%mol酒石酸酯得到最高80%e.e。

In this study, we have cloned and identified two full-length cDNAs of OsBIMK2 and OsBISERK1, which encoding MAPK and SERK, respectively, and associated with benzothiadiazole induced resistance.

本文克隆和鉴定了水稻中与苯并噻二唑(benzothiadiazole,BTH)诱导抗病性相关的编码MAPK的OsBIMK2基因和编码SERK的OsBISERK1基因全长cDNA;研究了OsBIMK2基因和OsBISERK1基因在水稻—稻瘟病菌亲和与非亲和互作过程中的表达模式;原核表达了OsBIMK2基因,获得重组OsBIMK2蛋白,并研究了重组OsBIMK2蛋白的生化特性;克隆了OsBIMK2基因的启动子序列,并运用农杆菌介导的瞬间表达系统分析鉴定了启动子中参与基因表达调控的可能顺式元件;将水稻OsBIMK2基因导入烟草,获得过量表达OsBIMK2基因的转基因植株,这些转基因植株中防卫反应基因PR-1组成型表达,并提高了抗病性。

The incorporation of selenocysteine needs a cis-acting element SECIS and four gene products: SelA, SelB, SelC and SelD.

研究表明硒代半胱氨酸的参入需要一个顺式作用元件SECIS和4个基因产物:SelA、SelB、SelC、SelD。

Usually, the unsaturated acids in nature oil exist in the cis-type. For example, vegetable oil, such as soybean oil, corn oil, olive oil and mustard oil, is rich in unsaturated fatty acids.

一般天然油脂中的不饱和脂肪酸多以顺式的结构存在,例如大豆油、玉米油、橄榄油及芥子油等植物油,都富含不饱和脂肪酸。

Therefore, one of central problems in biomedicine f...

因此,识别顺式作用元件并且预测它们的功能是生物医学领域的一个中心问题。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。

Its advantage is that it can be used in smaller units.

其优点在于可以在较小的单位中应用。