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Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠,融合筛选抗DR5杂交瘤细胞,制备抗DR5单抗——mDRA6;流式细胞术测定铂对HL60细胞表面DR5表达及细胞凋亡率;荧光显微镜下观察mDRA6与铂协同作用下HL60细胞形态变化;MTT法测定不同浓度的铂与mDRA6对HL60细胞存活的影响;琼脂糖凝胶电泳检测mDRA6与铂联合对HL60细胞DNA片段化的影响。

Maleic Anhydride is important organic raw material and the third biggest acid anhydride next to benzene anhydride and acid anhydride. In recent years, Maleic Anhydride is esterified and hydrogenised under pressure to make 1,4- butanediol butylene glycol, and it is also hydrogenised to tetrahydrofuran, which enables Maleic Anhydride to become the raw material for producing high-value-added products and to be more widely applied.

酐是重要的基本有机原料,也是世界上仅次于酸酐和苯酐的第三大酸酐,近年来,酐脂化低压加氢制1.4-丁二醇和酐加氢制四氢呋喃技术的开发成功,是酐成为生产高附加值产品的原料,应用领域有了更进一步的拓展。

Square德顺production fifteen years on the wine collection in full bloom in the small handless winecup firing imitation of porcelain, the white glaze, the antique blue printed with the "德顺Corner "in the words,...

2004年,东中国Sea酒复苏开始建立一种"百年德坊",我们的祖先那里继承下来,即使在今天的机械化广泛使用的传统做法酿造技术,所有的葡萄酒或基于纯手工制作葡萄酒,使辣土豆白药,天然健康草包制作音乐,使糯米酵母,混合了3惨败复制三个传统大酒缸前的固态发酵,。。。

"Bad couple" is about three times the women's high school comedy drama between sworn followers, in the drama as申恩庆fashion magazine editor-in-chief of the iceberg金棠son, CUI Jing-yun as doctors around the husband's typical full-time housewife Han iceberg,卞贞show three female students in the most straightforward character of a full-time housewives, as Luo石顺of the iceberg.

不良情侣》是讲述女子高中时代三个死党之间的喜剧电视剧,在剧中申恩庆担任时尚杂志主编金棠子一角,崔静允饰演围绕医生丈夫的典型专职主妇韩英一角,卞贞秀是三个女同学中性格最爽朗的一个专职主妇,饰演罗石一角。

Straight Flush同花顺 A straight flush is any five cards in sequence in the same suit.

同花就是任五张连续数字,同花色的牌。

We also showed that coadministration of AG825 or AG1478 will enhance the efficacy of cytotoxic AGent cisplatin.

了解5种人类非小细胞肺癌细胞系A549、H322、H358、H460、H1299的HER2和EGFR蛋白表达水平,探讨5种细胞系HER2蛋白表达水平与铂IC50之间的关系;观察HER2和EGFR小分子酪氨酸激酶抑制剂AG825和AG1478对5种细胞系的生长抑制作用;观察AG825、AG1478联合铂对5种细胞系的协同生长抑制作用;研究AG825、AG1478联合铂对H322、H1299细胞系细胞增殖、细胞周期、细胞凋亡和坏死的影响;研究AG825、AG1478联合铂所致H322、H1299细胞系EGFR、磷酸化ERK和总ERK表达的变化,探讨协同生长抑制作用的机制。

According to the order, the data of Caragana Korshinskii Kom is about 6.07 J/cm~2, 23.67 MPa, and 1787.98 MPa separately, and the data of Vitex is about 11.78 J/cm~2, 48.06 MPa and 3544.36 MPa separately. Additionally, the data of Fourfold Acacia is about 10.09 J/cm~2, 40.78 MPa and 2304.87 MPa , whereas the data of Grewia biloba var.parviflora is about 8.47 J/cm~2, 65.88MPa and 2523.88MPa respectively. The impact toughness four shrubs is in the bound of the data of wood in China, but the compressive strength and compression elastic modulus parallel to grain are some small than those of ordinary wood.

柠条的冲击强度为6.07J/cm~2,纹抗压强度为23.67MPa,纹抗压弹性模量(来源:AB75C论文网www.abclunwen.com)为1787.98MPa;荆条的冲击强度为11.78J/cm~2,纹抗压强度为48.06 MPa,纹抗压弹性模量为3544.36MPa;四倍体刺槐冲击强度为10.09 J/cm~2,纹抗压强度为40.78 MPa,纹抗压弹性模量为2304.87 MPa;扁担木的冲击强度为8.47J/cm~2,纹抗压强度为65.88MPa,纹抗压弹性模量为2523.88 MPa。

At first it analyzes the bedding rock slope"s bedded rock and its incontinuity face, and studies the stress, strain, stability"s condition of the bedding rock slope, fully considering the cohesive force of rock layer and incontinuity face.Geologic mechanics models of two types of bedding rock slope are set up, the instability coefficient of bedding rock slope under different mechanical parameters is computed, and the conclusion that the stability of bedding rock slope is relation to rock layerer, cohesive force of incontinuity face, inner friction angle and rock layer obliquity has been gotten.

本文首先对层边坡的层状岩体及其中的层理面和不连续面进行了分析,对其应力应变进行了研究,得出了层边坡的稳定性不仅与岩体中岩石的强度有关,还与岩体中层理面及不连续面的内聚力、内摩擦角及其倾角有关;论文建立了层理面和节理面的地质力学模型,讨论了其力学参数变化对边坡稳定性的影响,并在此基础上将层理面和节理面归纳为层边坡稳定性的结构面影响因素,同时讨论了影响层边坡稳定性的其他因素,从而得出层边坡稳定性的评价预测指标。

Recovery of maleic anhydride using nonaqueous agent is the direction of MA production.

非水溶剂法吸收丁烯二酸酐是酐生产的发展方向,为开发非水溶剂吸收酐的工艺,本文进行了酐在有机溶剂中的溶解度。。。

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果: hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙甙,对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙甙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙甙组相比,统计学上无显著差异(P>0.05)。hTERT反义核酸作用于CEM细胞24 h加入铂,再共同作用48 h,CEM活细胞均数为2.318×108 cells/L,与单用铂组(3.250×108 cells/L)及hTERT正义核酸联用铂组(3.175×108 cells/L)相比,对细胞抑制明显增强(P<0.05)。hTERT ASODN作用于CEM细胞24 h再加入铂作用48 h,细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与铂联合作用组(6.97%)、单用铂作用组(6.02%)进行比较有显著差异(P<0.01)。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?