鞭毛的

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Leishmania promastigotes antigens have good stability and heat-resistant nature. The sensitivity of 901 and 801 antigen were better, so we thought it could be used for serologic epidemiology investigation in XinJiang.

利什曼原虫前鞭毛体抗原有较好的稳定性，耐热性极好；901株、801株抗原的敏感性、特异性较好，可做为新疆血清流行病学调查所用的抗原株；801株的特异性更好，可用于黑热病的特异性抗体的检测及研究。

To investigate the function of STT3a gene in salt adaptation and flagellar regeneration of Dunaliella salina, a pair of degenerate primers was designed according to conserved homologous amino acid sequences of VCVFTA and DVDYVL of STT3a from Chlamydomonas, Arabidopsis thaliana and other organisms. A cDNA sequence of 1 650 bp encoding a whole functional domain of STT3a was amplified from D.

为了研究STT3a基因在杜氏盐藻耐盐及鞭毛再生方面的作用，根据衣藻、拟南芥等STT3a蛋白的氨基酸高度保守序列VCVFTA、DVDYVL设计一对简并引物，采用RT-PCR及3'RACE的方法扩增杜氏盐藻STT3a蛋白功能结构域的cDNA序列。

The locomotory apparatus that emerges during the spermiogenesis consists of the multilayered structure, basal bodies, flagella and accessory structures.

精子发生过程中形成的运动器的各部分结构包括鞭毛、基体、多层结构及附属结构；基体是细胞中新形成的结构，在不同类群的蕨类植物中分别由双中心粒、分支生毛体和生毛体产生。

The electron microscope micrograph demonstrated that E.coli has the internal region 11 cytoplasm which and the nuclein two may distinguish, gathers round a thin cytoplasma membrane outside them and the very thick cell wall, adheres to stick cohere some end dissociation outside the cell wall the flagella.

电镜显微照片显示E.coli有两个可以区别的内部区域一一细胞质和核质，在它们外面围着一层较薄的细胞质膜和很厚的细胞壁，在细胞壁外部附着一些一端游离的鞭毛。

The fatter mice in Gewirtz's study had been bred to lack a protein known as toll-like receptor 5 (TLR5), which most intestinal cells sprout on their surface. Its job is to recognize and bind to the whiplike flagella that bacteria use to move around. TLR5 acts as a traffic cop for controlling the mass of pathogens living in the intestine; without it, the normally harmless gut bacteria tend to overflourish and expand in number. See and listen to an audio slideshow about obesity rehab.

Gewirtz 实验中的胖小鼠普遍缺乏一种被称之为 toll 样受体5 (TLR5)的蛋白，这种蛋白是肠上皮细胞表面分泌的， TLR5的作用是识别并结合在肠道细菌的运动器官鞭毛上，在对大多数肠道病原体的控制中， TLR5扮演着交通警察的角色，没有它，一般无害的肠道菌就会异常活跃并且大量繁殖。

To exam Kala - azar patients, sera and healthy persons, sera from epidemic area.

目的用从新疆不同地区分离得到的利什曼原虫前鞭毛体提取抗原，观察ELISA法检测黑热病病人和疫区健康人血清抗体时的敏感性和特异性，为制备ELISA诊断试剂盒和进一步提高这种检测方法的敏感性和特异性，提高黑热病现场流行病学调查结果的准确性和可靠性提供实验依据。

We collected Leishmania promastigotes,washed its with 0.01mol/LpH7.2 buffers ,smashed its,and then measured protein content and titrated work concentration.2.We tested Kala-azar patients"serum and healthy persons"serum of epidemic area by ELISA. The results of three antigensmethods were the same, work concentration of serum was the same(l :100).Results:The protein content of three antigens is, 901 strain with 1000ug/ml,801 strain with 960ug/ml and 951 strain with 1100ug/ml. Suitable work concentration is, 901 strain with 10ug/ml ,801 strain with 12ug/ml and 951 strain with 11ug/ml.

1、抗原的提取：收集新疆不同地区分离得到的利什曼原虫前鞭毛体，经培养，用0.01mol/L pH7.2的磷酸盐缓冲液漂洗，超声粉碎，比色法测定其蛋白质含量，间接ELISA法滴定出抗原的工作稀释度。2、检测：用提取的三株抗原，分别进行间接ELISA法测定黑热病病人血清和疫区健康人血清抗体，并以肺结核病人血清、非疫区健康人血清做对照，三株抗原所用检测方法一致，血清工作稀释度均为1∶100。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。