鞭毛的

AIM: To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani anddetectexpressionofthegeneinNIH3T3cells.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

METHODS: Amastin gene was amplified from nuclear DNA of Leishmania Donovani isolates and cloned into an eukaryotic expression vector pcDNA31.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

Fungi are generally saprophytic or parasitic, and may be unicellular or composed of filaments that together comprise the fungal body or mycelium.

在它们的生活史的任一阶段都未发现鞭毛。

Vibrio s are defined as Gram-negative, asporogenous rods that are straight or have a single, rigid curve.

弧菌是革兰氏阴性、不产孢子的棒状或弧状的细菌，可以运动，许多弧菌在液体培养基条件下生长时具有一个极性鞭毛。

It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.

结果表明：蛭弧菌BDF-H16为革兰氏阴性菌，杆形或弧杆形，端生一根鞭毛，菌体大小多为0.2μm～0.5μm×0.8μm～1.2μm；蛭弧菌BDF-H16对实验所选用的革兰氏阴性菌和部分革兰氏阳性菌均有裂解作用；以大肠杆菌为宿主菌，其最佳生长条件为宿主菌浓度6.75×10~9cfu/mL、pH7.0～7.5、温度28℃；在NaCl含量0.85%～5.00%的培养基中能够生长；恩诺沙星、诺氟沙星对其有抑制作用。

It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.Its size was mostly 0.2μm～0.5μm×0.8μm～1.2μm.It had a wide prey area and could lyse all tested gram-negative bacteria and some gram-positive bacteria. The best lysis conditions to Escherichia coli were 6.75×109cfu/mL of prey bacteria concentration、pH7.0~7.5、28℃. It could grow in the solid culture added 0.85%~5.00% NaCl and was inhibited by enrofloxacin and norfloxacin.

结果表明：蛭弧菌BDF-H16为革兰氏阴性菌，杆形或弧杆形，端生一根鞭毛，菌体大小多为0.2μm～0.5μm×0.8μm～1.2μm；蛭弧菌BDF-H16对实验所选用的革兰氏阴性菌和部分革兰氏阳性菌均有裂解作用；以大肠杆菌为宿主菌，其最佳生长条件为宿主菌浓度6.75×109cfu/mL、pH7.0～7.5、温度28℃；在NaCl含量0.85%～5.00%的培养基中能够生长；恩诺沙星、诺氟沙星对其有抑制作用。

Fractionation of sperm proteins and in vitro polymerization of flagellar tubulins revealed that the protein might be one of the axonemal components of carp sperm.

后续的鲤鱼精虫蛋白萃取及鞭毛微管再组合实验则显示该蛋白可能是轴丝的成分之一。

Inhibition of PP1 gamma 2 causes motility initiation in immature spermatozoa and motility stimulation and changes in flagellar beat parameters in mature spermatozoa.

抑制PP1γ2会起始未成熟精子的运动并刺激和改变成熟精子的鞭毛运动参数。

Moreover, no significant changes in flagellar mobility, biofilm formation and production of extracellular cellulase and xylanase in vitro were observed in △rbfCxoo compared to PXO99A. Most importantly, the deletion mutation of rbfCxoo resulted in enhanced virulence and gene expression.

此外，△rbfCxoo鞭毛运动性、生物膜形成和胞外纤维素酶和木聚糖酶活性都无明显改变，但对水稻的致病性显著增强，毒性相关基因的表达也有所增加。

The unicellular alga Chlamydomonas offers a simple life cycle,easy culture and isolation of series of mutants,established the techniques and tool kit for molecular genetics and genetics analysis.It is now becoming the model organism for studies on photosynthesis in plant,flagellar assembly and function,cell cycle and circadian rhythms,signal transduction,light perception and cell recognition.

单细胞衣藻由于其生活周期简单，培养方法简便，易于分离得到系列的突变体，并已建立了分子遗传学研究技术与遗传分析系统，成为植物光合作用、鞭毛组装与功能、细胞周期及节律、细胞信号传导与光感受、细胞识别等重要生物学过程研究的模式生物体。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。