鞭毛

ConclusionThe concentration and handing time of suspension, slidemaking method and staining time are important factors for an ideal flagellum staining.

细菌鞭毛染色过程中菌悬液的浓度、菌悬液的处理时间、制片方法以及染色时间的长短等条件影响鞭毛染色效果。

ConclusionThe concentration and handing time of suspension, slide making method and staining time are important factors for an ideal flagellum staining.

细菌鞭毛染色过程中菌悬液的浓度、菌悬液的处理时间、制片方法以及染色时间的长短等条件影响鞭毛染色效果。

Using special stains, one can see that some bacteria have attached to them wavy-looking "hairs" called flagella.Others have only one flagellum.

使用特殊的着色剂后，你会发现有的细菌上长着不少波状的"毛发"即鞭毛，而有的细菌只有一根鞭毛。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

Objective To detect the expression level of stage-specific genes in Leishmania promastigotes and amastigotes.

摘要目的证明亚马孙利什曼原虫前鞭毛体和无鞭毛体的基因表达水平。

These findings lead to an MSAP/septin-mediated model for flagellar differentiation during spermiogenesis.

免疫萤光定位分析显示septin7与MSAP共同位於精虫鞭毛的基底体上。septin蛋白家族可能参与细胞质分裂及细胞形态生成等事件，因此推测MSAP/septin复合体的功能可能涉及鲤鱼精细胞之极性建立及鞭毛形成。

The samples were smeared on slides and stained with Giemsas stain. The stained smears were observed microscopically and photographed. It showed that except typical trophozoites in binary fission. the following trophozoites of abnormal morphs were also observed. for instance abnormal trophozoites with binary fission; enlarged trophozoites with round and irregular shaped eosinophilic bodies in the cell plasma; enlarged cells containing round and irregular shaped eosinophilic bodies and flagella; enlarged cells which contain 6 or 8 nuclear-like bodies and flagella, 3 or 4 embryonic forms of daughter trophozoite in one mother trophozoite; 4 trophozoite embryonic forms with fused plasma 3 trophozoites with fused plasma;4 trophozoites with fused plasma; a pair of trophozoite with binary fission fused with another trophozoite;2 pairs of trophozoite with binary fission fused each other; a trophozoite with 1 nuclear only.

结果 除观察到典型的营二分裂法繁殖的贾第虫滋养体外，还可见到多种形态异常的虫体，包括；虫体呈非典型二分裂；滋养体胀大，胞质中有团块状和不规则形状的嗜酸性物质；在胀大的贾第虫细胞内含有团块状和不规则形状的嗜酸性物质以及鞭毛；在胀大的贾第虫细胞内含有6或8个核状物以及鞭毛；在一个母体细胞中含有3或4个子体细胞的雏形；胞质互相融合的4个滋养体的雏形；胞质互相触合的3个滋养体，胞质互相融合的4个滋养体；1对营二分裂的滋养体与另一个滋养休互相融合；2对营二分裂的滋养体互相融合；仅有1个细胞核的滋养体。

Methods: We generated amastigotes via in vitro transformation from promastigotes and maintained amastigotes in axenic culture using a modified protocol by increasing the FBS from 20% to 50%.

我们通过改良培养基，将小牛血清从20%提高到50%，在体外将前鞭毛体转化为无鞭毛体，并在体外纯培养。

Objective To explore the protein profile and identify developmentally regulated proteins of the promastigotes and axenic amastigotes with comparative proteomics technique.

摘要目的应用蛋白质组学技术分析杜氏利什曼原虫前鞭毛体和无鞭毛体蛋白质表达状况。

Results Approximately 700 protein spots were revealed in equivalent proteins of the promastigotes and axenic amastigotes separated by 2-DE,among which more than 90% protein spots showed equivalent quantity and distribution,with 6 proteins up-regulated and 3 proteins down-regulated in axenic amastigotes compared with promastigotes.

结果 等量的前鞭毛体与纯培养的无鞭毛体总蛋白经双向电泳分离后均可获近700个蛋白点，其中超过90%的蛋白点的分布和相对强度基本一致。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。