鞘氨醇

During these years (1997-2000) in the research area of annonaceous acetogenins 5 natural products and a number of chiral-pure analogs of annonaceous acetogenins have been synthesized using sugars as the starting materials. Their high activity and selectivity against cell line of colon tumor and a relationship between activity and configuration have been found too. In the respect of inhibitor of sialidase two new methodologies for the synthesis of chiral-pure 3-deoxy-ulosonic acid using hetero-Diels-Alder reaction or asymmetric propargylation as the key reaction were developed. On the topic of anti-leukemia marine natural product clavulactone a synthetic method based on the free radical cyclization and starting from sugar has been developed. In other title of PKC inhibited agent sphingosine a new synthetic route with SN2' as the key reaction has also been disclosed.

项目期间（1997-2000）在番荔枝内酯方面从糖出发合成了五个天然产物和一批手性纯的类似物，它们对肠癌细胞有很高的活性和选择性，并与构型有关；在唾液酸酶抑制剂方面发展了二类全新手性纯高碳糖的合成方法－杂原子D－A反应或丙炔基不对称加成为关键的反应；抗白血病海洋天然产物群柱虫内酯方面研究了由糖出发和自由基环化反应的合成方法；对PKC 起抑制作用的鞘氨醇方面也开创了新的合成途径－由糖出发并以SN2'反应为关键反应。

By suspending cell culture from the callus of P. thugbergii and by fluorescent observation, a new method, which is easy and sensitive to observe cell activity and to test the phytotoxicity of various bacteria, was used. This method can be utilized to study the bacterial toxins. Cultured filtrates of some strains of the nematode-carrying bacteria were bioassayed for their toxicity to suspending cultured cells from P. thugbergii callus. Strong toxic effects were observed by the filtrates of Pseudomonas spp., P. fluorescence, and Sphingomonas spp., these bacteria species are coincide with those in the early stage of pine wilt disease.

本试验使用了黑松愈伤组织的细胞悬浮培养方法，用荧光方法观察各种细菌对植物细胞的毒性测定，这种方法具对细胞的活性观察容易、灵敏度高等特点，证明可用于细菌的毒素研究；对松材线虫携带的细菌的部分菌株，用其培养滤液对黑松愈伤组织的悬浮培养细胞的生物测定初步证明，松材线虫携带的细菌中以假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性，这与松树发病早期出现的细菌种类相一致。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。