靶

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

In this dissertation, the mechanism of RNAi and basic principle and methods are used to analyze the distributing of reverse complementation sequence correlated with RNAi. It is presumed that a genetic regulation network related with endogenesis RNAi is in existence.

本文根据RNA干扰的基本原理，运用生物信息学的原理和方法，通过计算机编程寻找和RNA干扰相关的反向互补序列，在各种模式生物基因组中找到大量发夹—靶点作用对，并统计了这些序列在各种模式生物基因组中的分布情况，推测存在一个由内源性RNA干扰参与的一个基因调控网络。

The study of triazole derivatives targeted IGPD is mainly reviewed, the enzymology and catalytic mechanism are also described in this paper.

文中介绍了IGPD的酶学特征与催化机理，并着重概述了以IGPD为靶标的三唑磷酸酯类抑制剂的研究进展。

The vapour and plasma ignition times of the target are got based on the equation of heat conduction and cascade model. We suppose that the plasma is ignited when 210 generations of new electrons are born in vapour generation time. The influence of temperature on the thermodynamics and optics parameters of material also have been considered in the model. The resulting theory has good agreement with the experiment and overseas report. A blade method to measure the laser spot is given in this paper with validation and error analysis. The plasma threshold of metals in atmosphere and water ambients both are diagnosed with the light deflection and piezoelectric transducer. A Q-switched pulsed Nd:YAG laser operating at infrared (1064nm), visible(532nm) and ultraviolet (355nm) wavelengths has been used. Al、Fe、Cu are used as targets and get a similar results with both method. Theoretical and experimental analyses are applied on the influence of wavelength on the threshold have been done, both of which have shown that the plasma threshold of metals decrease as the laser wavelength increases; The plasma threshold of metals are higher in water than in air and the pressure of the shock wave in water is five times higher than in air.

从热传导方程和雪崩电离机制出发，假设当电子增值2~(10)倍时，考虑了温度对材料热力学和光学参数的影响时，得到了气化和等离子体点燃的时间，利用该模型进行计算得到的结果与国内外报道及自行通过实验测得的阈值基本一致；提出了利用刀刃法测量激光光斑面积的方法，并通过实验进行了验证和误差分析；利用光偏转装置和压电换能器分别对空气和水中金属等离子体点燃阈值进行了实验诊断，激光器均为调Q—YAG激光器(波长1064nm，532nm，355nm，脉宽10ns)，靶材分别为Al、Fe和Cu，两种测试方法得到的等离子体点燃阈值基本一致；本文从实验和理论计算两个方面讨论了波长对等离子体点燃阈值的影响，均得到了等离子体点燃阈值随着波长的增加而减小的结论；对空气中和水中不同环境下金属等离子体的点燃阈值进行了比较研究，得到了金属在水中的等离子体点燃阈值比空气中的大，且水中产生的冲击波的压强是空气中的5倍左右的结论。

And so they proceeded to invent the verge-and-foliot escapement,using a falling weight as with its Greek antecedents.

翻译界早已认识到，翻译水平不只是对源语文的理解力和对靶语文的表达力，还包括所译专业的知识水平

Fragments of exon 2,5,17 and 20 of BRCA1 gene were amplified by PCR.

BRCA1还是某些特异靶基因的转录调控子〔2〕。

Sequence encoding Pseudomonas exotoxin A translocating peptide was localized between antibody gene and GrBa gene.

表达的immunoGrB蛋白进入肿瘤靶细胞的内吞小体后，发生Arg279和Gly280之间肽键的定点裂解，裂解后的PE Ⅱ-GrBa被转位到细胞质中。

From experimental and calculated results, X-ray transport attenuation changes in exponential function, and scaling law for radiation transport is obatined.

由实验和计算的结果推断X光在管靶中输运是指数衰减的，由数值计算结合实验结果得到辐射输运的定标公式。

Aiming at improvement of attack precision and reduction of target miss distance, different guidance and control laws are proposed and analyzed, the influences of many different kind of conditions to attack precision such as initial flying altitude, airspeed, flight path angle, external disturbance and target movement are investigated. Beneficial to theoretical significance and inspiring for engineering practice results are obtained.

紧紧围绕提高末制导精度、减小脱靶量这一研究目的，对不同的末制导控制方式、不同的末制导导引律、不同的末制导进入条件、不同的外部干扰、不同的目标特性等都进行了深入的研究、分析和探讨，并给出具有一定理论意义和丰富的工程实际应用价值的结论。

Anatipestifer existed in Jiangsu province and bursa of Fabricius was the primary target organ.

结果表明，江苏地区存在新的RA血清型，法氏囊是该细菌感染的一个重要靶器官。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。