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Gram ' s stain A stain containing the basic dye crystal violet or gentian violet that forms the basis for the division into Gram positive and Gram negative bacteria.

革兰氏染色法:是一种使用结晶紫或龙胆紫作为染料来区分革兰氏阳性菌和革兰氏阴性菌的染色方法。

Similarly, a previous gram-positive case did not reduce severity of a second or third gram-positive case.

同样,前革兰氏阳性案例并未减少严重的第二次或第三次革兰氏阳性案例。

It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.

结果表明:蛭弧菌BDF-H16为革兰氏阴性菌,杆形或弧杆形,端生一根鞭毛,菌体大小多为0.2μm~0.5μm×0.8μm~1.2μm;蛭弧菌BDF-H16对实验所选用的革兰氏阴性菌和部分革兰氏阳性菌均有裂解作用;以大肠杆菌为宿主菌,其最佳生长条件为宿主菌浓度6.75×10~9cfu/mL、pH7.0~7.5、温度28℃;在NaCl含量0.85%~5.00%的培养基中能够生长;恩诺沙星、诺氟沙星对其有抑制作用。

It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.Its size was mostly 0.2μm~0.5μm×0.8μm~1.2μm.It had a wide prey area and could lyse all tested gram-negative bacteria and some gram-positive bacteria. The best lysis conditions to Escherichia coli were 6.75×109cfu/mL of prey bacteria concentration、pH7.0~7.5、28℃. It could grow in the solid culture added 0.85%~5.00% NaCl and was inhibited by enrofloxacin and norfloxacin.

结果表明:蛭弧菌BDF-H16为革兰氏阴性菌,杆形或弧杆形,端生一根鞭毛,菌体大小多为0.2μm~0.5μm×0.8μm~1.2μm;蛭弧菌BDF-H16对实验所选用的革兰氏阴性菌和部分革兰氏阳性菌均有裂解作用;以大肠杆菌为宿主菌,其最佳生长条件为宿主菌浓度6.75×109cfu/mL、pH7.0~7.5、温度28℃;在NaCl含量0.85%~5.00%的培养基中能够生长;恩诺沙星、诺氟沙星对其有抑制作用。

Although cholestasis of sepsis is often associated with gram-nagetive bacterial infection,it also occurs with a diverse array of other organisms,including Gram-positive bacteria,rickettsial,parasitic and fungal infections.in one study of bacteraemic patients with positive blood cultures only 34% had bilirubin levels greater than or equal to 2.0mg/dl.moreover,in clinical practice the frequency and extent of liver enzyme abnormalities in bacteraemic patients may be far greater than the occurrence of hyperbilirubinaemia.cholestasis appears to be a marker for the severity of sepsis,and prolonged and unresolved cholestasis carries a high mortality.

虽然败血症(sepsis引发的胆汁郁积往往与革兰氏阴性菌(gram-negative bacterial,原文negative瓶错了)感染,它也可能伴随各种各样其他的有机体,包括革兰氏阳性菌(Gram-positive bacteria),立克次体属微生物,寄生虫和真菌感染。一个对菌血症(bacteremic,原文中拼错了)的阳性患者的血液培养分析研究发现其中只有34%的胆红素水平大于或等于2.0mg/dl。而且,在临床实践中的血症患者肝酶异常的发生频率和严重程度可能远远大于高胆红素血症(hyperbilirubinemia,原文中拼错了)。胆汁郁积似乎是一个患有严重性败血症的标志,而且长期的和没有根治的胆汁郁积有很高的死亡率。

It was seen that the main microflora causing the spoilage of fresh mutton meat consisted of 3 gram-positive bacilli with spore (about 30% of the micro flora), 1 gram-positive coccus (about 2% of the whole microflora), 3 gram-negative spirilla (about 63% of the whole microflora), and 1 gram-negative bacillus (about 5% of the whole microflora). Potassium sorbate, EDTA and nisin were used as preservatives to inhibit the growth of the main strains representative for the spoilage of fresh lamb meat.

对屠宰后羊肉的微生物区系进行了调查,成功地分离出引起冷却羊肉腐败的主要革兰氏阳性菌和阴性菌8种,其中含芽孢的革兰氏阳性杆菌有3种,占整个微生物区系的30%;革兰氏阳性球菌1种,占整个区系的2%;革兰氏阴性螺旋杆菌3种,占整个区系的63%;革兰氏阴性杆菌1种,占整个区系的5%。

It was found that 0.25%o potassium sorbate produced a positive inhibition against short G+ spore bacillus with a concentration less than 5xl04cfu/ml. A concentration less than %o potassium sorbate hardly exerted a complete control towards short G- plump bacillus having a population density of 5x104cfu/ml. It was proved that use of l% potassium sorbute never controlled the growth of G+ coccus, G~ spirilla and enterobacter with a population density of 104cfu/ml. 1mmol EDTA completely controlled the growth of G+ short spore bacillus and G+ coccus whose cell density was 5xl04cfu/ml. A level of lmmol EDTA showed a limited inhibition against the growth of G- spirilla with a population density of 105 cfu/ml. However, a level of 10mmol EDTA completely controlled the growth of the G- spiral bacteria having a population density of 105cfu/ml. lOmmol EDTA produced a very significant control towards the growth of G"" plump short bacillus with 105cfu/ml. 20mmol EDTA showed a remarkable inhibition against the enterobacter with a population density of 105cfu/ml. Different concentrations of nisin including 25mg/mL, 50mg/mL, 75mg/mL and 100mg/mL were used as bio-preservative to examine its effects against the growth of all strains leading to the spoilage of fresh mutton meat. It was seen that there was a big difference in nisin's concentrations in inhibiting the spoiling bacteria. Generally speaking, as more as 75mg/mL of nisin significantly inhibited the growth of G+ short spore bacillus, G-plump short bacillus, enterobacter, G'spiral bacteria and G+ coccus having a population density of 105cfu/ml.

分别运用山梨酸钾、EDTA和Nisin对7种主要引起羊肉腐败的微生物进行了抑菌实验,结果显示,0.25‰以上山梨酸钾能够有效抑制5×10~4 cfu/mL以下的革兰氏阳性短芽孢杆菌的生长;1‰以下的山梨酸钾不能完全抑制5×10~4 cfu/mL革兰氏阴性粗短杆菌的生长,对10~4 cfu/mL革兰氏阳性球菌菌株、革兰氏阴性螺旋菌菌株和肠杆菌菌株抑制效果不太明显。1mmoL EDTA能完全抑制住小于10~5 cfu/mL革兰氏阳性短芽孢杆菌菌株、革兰氏阳性球菌菌株的生长,能明显的抑制10~5 cfu/mL的革兰氏阴性粗短杆菌菌株生长,对10~5 cfu/mL的革兰氏阴性螺旋菌菌株有一定的抑制作用。10mmoL EDTA能完全抑制住10~5 cfu/mL革兰氏阴性螺旋菌菌株的生长;能明显抑制10~5 cfu/mL的革兰氏阴性粗短杆菌菌株的生长,而20 mmoL EDTA能很明显抑制10~5 cfu/mL肠杆菌菌株的生长。25mg/mL、50 mg/mL、75 mg/mL和100 mg/mL的Nisin几乎对所有引起羊肉的腐败菌有抑制作用,但抑制程度不同,抑菌活性有一定的变化。

Maintypes of spoilage bacteria were incubated with selective agars to analyze the antisepticeffect of antimicrobials. The result showed: in the factor of antiseptic effect, nisin>lysozyme>lactic acid sodium>chitosan>tea polyphenol.

结果表明:5种保鲜剂的综合抑菌效果依次为Nisin>溶菌酶>乳酸钠>壳聚糖>茶多酚;Nisin和溶菌酶对革兰氏阳性的乳酸菌和热死环丝菌有很好的抑制作用,而对革兰氏阴性的假单胞菌属和肠杆菌科的抑制作用较差。

Of, relating to, or being a bacterium that retains the violet stain used in Gram's method .

革兰氏阳性的属于,有关或作为使用革兰氏染色法时保留紫色色素的细菌的

A positive test for pregnancy.Of, relating to, or being a bacterium that retains the violet stain used in Gram's method.

革兰氏阳性的属于,有关或作为使用革兰氏染色法时保留紫色色素的细菌的

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