革兰氏法

Gram ' s stain A stain containing the basic dye crystal violet or gentian violet that forms the basis for the division into Gram positive and Gram negative bacteria.

革兰氏染色法：是一种使用结晶紫或龙胆紫作为染料来区分革兰氏阳性菌和革兰氏阴性菌的染色方法。

The orange osmanthus skin-traditional Chinese medicine being taken as raw material, the active ingredient-phenol was extracted with the method of steam distillation and then the derivative of paeonol-aminoethanol and 2,4-dinitrophenyl hydrazone was synthesized. The structural characterization of the derivatives carried out was identified and the test in the anti-bacterial role of three kinds of gram-negative and positive bacteria was done with the method of agar diffusion.

方法］以丹皮为原料，用水蒸气蒸馏法从中药丹皮中提取活性成分丹皮酚，进而合成丹皮酚缩乙醇胺和2，4-二硝基苯腙衍生物，对衍生物进行了结构表征，并采用琼脂扩散法，对3种革兰氏阴性菌、阳性菌进行了抑菌性试验。

Objective To establish a simple,accurate and practi cal method of extracting plasmid from gram-negative and gram-positive bacteria ,Method we selected,Biswajit sahas method and comported others method, such as controlling pH andtemperature strictly and increasing EDTA-Na2 capacity.

为建立一种革兰氏阴性及阳性菌均能分析的质粒提取方法，以Biswajit saha的方法为基础，对比酸酚法、热变性法、碱变性法、酶法等方法，研制符合国内一般实验室的方法。

Objective To explore the clinical value of the titer of serum HP antibody in patients with gastric diseases.Methods Serous HP antibody was detected with ELISA and HP was cultured,tested with urase,and stained with Gram method on smears which was observed microscopically and compared with proteus protein-enveloped test.Results ELISA showed high sensitivity and specificity.Conclusion Serous ELISA detection of HP antibody can be used widely in clinical diagnosis and epidemical screening without endoscopy and biopsy.

目的 探索胃部疾病患者血清中的幽门螺旋菌HP抗体滴度的临床价值方法应用酶联免疫吸附试验ELISA法检测HP抗体与HP培养，尿素酶试验和涂片革兰氏染色作镜下形态检查相比较，并同时与变形杆菌蛋白包被测定法作比较结果显示该法具有敏感特异性强的特点结论该法无需常规内镜检查，活检查HP而单纯用血清学检查，可广泛应用于临床诊断和流行病学人群普查

A bacteriocin-like substance producing strain T12 was screened from 212 lactic acid bacteria isolated from intestine of healthy pigs by paper disk test and was identified as Lactobacillus. The supernatant of T12 exhibited strong inhibitory activity against the gram-positive bacteria, including Micrococcus lysodeikticus, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus. A strong activity was also observed against the gram–negative bacteria and some eumycete, such as Escherichia coli, Salmonella typhimurium and Fusarium graminearum.

通过滤纸片法从健康肉猪猪大肠、小肠中分离得到212株抗生物质产生菌，以杯碟法复筛，得到1株对溶壁微球菌、金黄色葡萄球菌、枯草芽孢杆菌、蜡状芽孢杆菌等革兰氏阳性菌和大肠杆菌、鼠伤寒沙门氏菌等革兰氏阴性菌以及部分真菌如禾谷镰刀霉均有强烈抑制作用的乳酸菌。

The central theme of this thesis is to investigate the expression of lymphocyte surface marker and concentration of serum cytokines in four groups of patients with sepsis : Group 1, patients with Gram positive bacterial infection; Group 2, patients with Gram negative bacterial infection; Group 3, patients with negative cultures but strong evidence of infection; Group 4, patients without clinical and microbiologic evidence of infection as control group.

本研究主要以临床诊断为主再配合血液培养将病人分为四组：第一组是有革兰氏阳性细菌感染的病人；第二组是有革兰氏阴性细菌感染的病人；第三组是没有培养出细菌的感染性病人；第四组是由临床诊断及微生物培养证实为正常对照组。利用单株抗体接上萤光物质再配合流式细胞分析仪来测定淋巴球上各种细胞膜表面分化抗原的变化及利用酵素免疫分析法来探讨血清各种细胞素之变化。

This study re-evaluated the application of Gram's stain in diagnosing gonorrhea in women by comparing with gonococci culture.615 samples from 500 cases in clinic of Gynecology were studied.

本文用淋球菌培养比较研究对革兰氏染色法在女性淋病诊断中的应用作重新评价。

Of, relating to, or being a bacterium that retains the violet stain used in Gram's method .

革兰氏阳性的属于，有关或作为使用革兰氏染色法时保留紫色色素的细菌的

Of, relating to, or being a bacterium that does not retain the violet stain used in Gram's method .

革兰氏阴性的属于，有关或作为用革兰氏法染色不保留紫色色素的细菌的

A positive test for pregnancy.Of, relating to, or being a bacterium that retains the violet stain used in Gram's method.

革兰氏阳性的属于，有关或作为使用革兰氏染色法时保留紫色色素的细菌的

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。