非限制性的

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

The newly constructed nano-gold-baesd gene chip combined with restrictive enzyme digestion without PCR can be widely applied in the simulta- neous detection of Mycoplasma, Chlamydia, fungus, virus and bacteria. It shows a bright prospect in increasing the throughput of identi- fieation of pathogene.

本研究构建的基于限制性酶切非PCR法的纳米金芯片技术可以同时实现对细菌、真菌、支原体、衣原体及病毒等微生物的检测，适用性广且对大幅度提高检测通量有积极意义。

Aphids in the process of inhaling plant sap virus particles and auxiliary proteins also be inhaled, thus completing the process by poison, which also supports the field citrus aphid is non-persistent decline disease vector of disease, while the citrus Huanglongbing is phloem restricted by the bacteria caused the main pathogens present in the phloem, the fleas only by phloem feeding in diseased plants, pathogens in the body through the back to complete the salivary glands in the course of the next through the sieve tube feeding in the secretion of saliva the pathogens into the healthy tree, Citrus Huanglongbing different characteristics with aphid-borne diseases, Huanglongbing to persistent disease.

蚜虫在吸入植物汁液的过程中病毒粒子和辅蛋白同时也被吸入，从而完成获毒过程，这也支持了田间蚜虫是柑桔衰退病等非持久性病害传播介体，而柑桔黄龙病是由韧皮部限制性细菌类引起，病原体主要存在于韧皮部中，木虱只有通过在病株韧皮部取食，病原在体内完成循回到唾液腺中，在下一次取食过程中通过在筛管中分泌的唾液将病原体带到健树，柑桔黄龙病与蚜虫传病特点不同，黄龙病为持久性病害。

Based on these results and inferred to related reports from other labratories, it was possible to make some analyses and conclusions or inferrences:(1) CD〓AK with tumoricidal activity were induced and expanded in number througth costimulation of PBMC with anti-CD〓 McAb . and r IL-2 ;(2) CD〓AK induced and expanded in such manner did exibit more potent proliferation·ability and cytotoxicity which maintained for lonser time than those of LAK cells, thus CD〓AK was a new variety of antitumor effector cells worth to be explored;(3) CD〓AK could mediate MHC nonrestricted cytotoxicity and kill tumor target cells through inducing necrosis and apoptosis;(4) Normal mature lymphocytes of PBMC could be induced to proliferate and /or to die from apoptosis when they were costimulated by anti-CD〓McAb and rIL-2. Both proliferation and apoptosis were existing in the same cultivation system sugsesting that the presence of rIL- 2 might provide some accessary signals for apoptosis.

以这些结果为基础并参考其它有关文献可能做出如下分析与结论或推论：(1)用抗CD〓单抗和rIL-2共刺激外周血单个核细胞能诱生扩增出具有杀瘤活性的CD〓AK细胞，(2)与LAK相比，用这种方法诱生扩增的CD〓AK增殖能力强、细胞毒活性强而且维持时间长；CD〓AK是一类值得开发的抗瘤效应细胞；(3)CD〓AK能够介导MHC非限制性细胞毒活性，可以通过诱导靶细胞坏死和/或凋亡杀伤肿瘤细胞；(4)正常外周血单个核细胞中的成熟淋巴细胞在受到抗CD〓单抗和rIL-2共同刺激后既可诱导增殖也可诱导凋亡，两者并存于同一体系，推测rIL-2的存在可能为细胞凋亡提供一些辅助信号。

The otological, audiological and neurological examinations of these patients were conducted, including otoscopy, pure-tone (Madsen 522) and immittance(GSI 33) audiometry. MtDNA A1555G mutation and GJB2 235delC mutation were detected with specific restriction enzyme digestion methods.

对 148 名非综合征型感音神经性耳聋患者分别进行线粒体 DNA 12SrRNA基因A1555G 点突变和 GJB2 基因235delC突变的限制性内切酶分析。

Good balance of joint space can be achieved by the suitable adjustment of the soft tissues, so the unrestrictive prosthesis is used for the valgus patients, and the knee joint will be stabilized without the presence of cruciate ligament excision.

对于膝外翻的患者，如果合理的调整软组织获得良好的关节间隙平衡，就可以采用非限制性假体，不切除交叉韧带，获得膝关节的稳定。关键词：膝关节置换术；膝外翻；生物力学；探讨

The frequency of three mitochondriai point mutations,1555A→G,3243A→G,7445A→G,was examined using restriction fragment length polymorphism annlysis in 262 Chinese patients with NSHL,con- sisting of 168 sporadic cases,69 sib pairs,and 25 families with affected subjects in more than one generation.

方法收集非综合征型神经性耳聋病例262例，其中散发病例168例，69例同代有耳聋患者，25例不止1代有耳聋患者，采用限制性片段长度多态性分析线粒体基因中的下列3个点突变：1555A→G，3243A→G，7445A→G。

On this day of 1787 – Shays' Rebellion, an armed uprising in central and western Massachusetts, was crushed, an event that energized calls in the United States for a stronger government than what was established by the Articles of Confederation.

您分析的很好！和我想的一样！问题是：在这个case下，非限制性定语从句可以省略吗？好像见的不多啊

This variation may be a genetic factor for obese subjects in China to develop type 2 diabetes mellitus.

近年来报道表明，位于3′非转录区上的内切酶SacII的限制性片段长度多态性与

Methods: fifty-three healthy subjects, 105 subjects with simple obesity, 63 type 2 diabetic patients without obesity, and 114 type 2 diabetic patients with obesity were studied with the technique of pcr- rflp in codon 64 of the exon region of β3-ar gene representing the variation trp/arg.

运用多聚酶链反应-限制性片段多态性分析技术分析了53名正常对照者、105名单纯性肥胖患者、63名t2dm非肥胖患者和114名t2dm伴肥胖患者的β3-ar基因外显子64位色氨酸、精氨酸的多态性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐