非酶的

We find the deep Y-shaped hydrophobic pocket spans SPE16 and the hydrophobic patch at the SPE16 surface binded apolar ligands ANS in SPE16 .

SPE16复合物的结构显示存在于SPE16结构中的Y型疏水空穴和分子表面的疏水区有结合非极性分子的功能，显示SPE16蛋白除了RNase酶活性以外的结合功能。

Progress in cloning techniques, microbiology and protein purification has been supplying an ever-growing number and quantity of preparatively useful biocatalysts. Initially enzymes were only used to synthesize compounds found in nature.

近年来人们在克隆技术，微生物学以及蛋白纯化方面的研究成果为我们提供了越来越多的生物催化剂，酶最初用于天然产物的合成，而今其在非天然化合物中的应用更引起了生物化学家的广泛关注。

Results: Caspase-3 mRNA was constitutively expressed at a low level in freshlyisolated CD34+ cells. The expression of caspase-3 mRNA and protein was upregulated when these cellswere first expanded in suspension culture with growth factors for 3 days. However, only the 32 kDa inactivecaspase-3 proenzyme was detected in the freshly isolated CD34+ cells as well as during the first 3 daysexpansion with cytokines.

检测结果显示，Caspase-3mRNA在新鲜分离的脐血CD34+细胞中低水平表达，在细胞因子支持下体外培养3天，扩增的CD34+细胞中Caspase-3mRNA和蛋白质水平表达上调，但在这两种细胞中仅能检测到分子量为32KDa的非活性酶原形式的Caspase-3；随着体外培养时间的延长，在IL-3，IL-6和GM-CSF组合条件下，Caspase-3被激活，可检测到分子量为20KDa的裂解片段。

The results show that homogeneous collagen dispersion can be gained by both methods. But only by the second method can we receive typical cross striation structure which was observed by EM. The solid content and viscosity of the collagen dispersion can be easily controlled.

结果表明：两种方法均能得到均匀的溶胀体，但用酸法提取的材料电镜观察为非横纹结构；而酶消化法提取的材料，电镜照片为典型的横纹结构，胶原溶液的固体含量、粘度等指标可控制。

In this study, we analyzed 8 rare and endangered animals, including giant panda, Yunnan golden monkey, red panda, Asiatic black bear, rhesus monkey, slow lorries, Chinese pangolin and concolor gibbons from China and adjacent areas.

本文以我国珍稀动物大熊猫、滇金丝猴、小熊猫，亚州黑熊、间蜂猴、恒河猴、中国穿山甲、黑冠长臂猿为材料，采用蛋白和同工酶电泳以及线粒体DNA序列分析（包扩采用非损伤取样以毛发和陈旧皮张样品进行的分析）的方法研究珍稀动物的遗传多样性状况及其同物种濒危的关系以及根据遗传学数据确定保护的基本单元等。

In contrast to conventional methods, a novel cell sheet-based technique has been proposed .This technique intend to achieve high cell density and function by layering confluent viable cell sheets without the use of acellular scaffolds that limit mass transport. Using thermo-sensitive cell culture surfaces, confluently cultured cells spontaneously detach as intact sheets simply by reducing the culture temperature.

与传统方法相比，有学者提出了一种新的以细胞层为基础的组织工程技术，这种技术作为一种新的选择，利用温敏性培养皿，经简单的温度变化，可以非损伤性的一起获得完整的培养细胞层及其所沉积的细胞外基质，从而避免了使用蛋白水解酶。

In this study, the ovarial cancer stem cells were successfully obtained through the suspension culture of the ovarian cancer 0VCAR3 cell line in serum-free media. The stem cells formed spherical colonies, called "ovarispheres", and showed anchorage-independent ability. Furthermore, the bionomics and morphology of the stem cells were observed, and the expression difference of the tumor stem cells marker-ABCG2 between ovarispheres and anchorage-dependent cells was identified by using RT-PCR and Immunocytochemistry methods.

本研究通过无血清悬浮培养人卵巢癌细胞系OVCAR3，获得具有非锚着依赖能力的肿瘤干细胞球，观察其生物学特性及形态学的变化，并采用半定量逆转录—聚合酶链反应及细胞免疫化学的方法鉴定肿瘤干细胞标志ABCG2在贴壁细胞及肿瘤干细胞球中的表达差异。

The non-specific immune functions were studied in four populations of Portunus trituberculatus from Haizhou Bay, Dandong, Zhoushan and Laizhou Bay for the immunity enzyme activities in haemolymph, muscle and hepatopancreas tissue of the crabs. Results showed that SOD activities of haemolymph and muscle were not significant different by four populations.

本文测定了海州湾、辽东湾、舟山沿海和莱州湾4个野生群体三疣梭子蟹的血淋巴、肌肉和肝胰腺组织中的抗氧化及其它与非特异性免疫相关的酶活指标，并对这些指标在不同群体间的差异进行了比较。

An additional metabolic characteristic associated with the growth and toxin production of Clostridium botulinum is gas production.

另外与肉毒杆菌的生长与产毒相关的代谢特征是气体的产生。许多酶如不外加一种叫做辅助因子的非蛋白质物质就不能工作。

To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。