非酶的

The extent of inhibition of depolarization by superfusion of ethanol for 5 minutes was 41.8±3.3 ％(n=17). The addition of 5 nM and 20 nM KT5720 (a selective protein kinase A inhibitor) and 250 μM H9 dihydrochloride (a non-selective protein kinase inhibitor), into the intracellular solution significantly attenuated the inhibitory effects of ethanol.

当分别将5 nM或20 nM KT5720（选择性蛋白质激酶A抑制剂）或250 μM H9 dihydrochoride（非选择性蛋白质激酶抑制剂）置入细胞内液时，乙醇的抑制作用明显被减弱；但乙醇的抑制作用不受到5 nM或20 nM calyculin A（磷酸酶1/2A抑制剂）的影响。

In this study, we investigated the effects of 1.0 and 2.0 minimumalveolar anesthetic concentration sevoflurane on cerebralblood flow autoregulation before and after nonselectiveinhibition of nitric oxide synthase in rats.

在此研究中，我们观察了应用非选择性一氧化氮合酶抑制剂前后，1.0和2.0最小肺泡浓度的七氟醚对大鼠的脑血流自体调节的影响。

Aim of the study To inestigate the effects of citric acid addition, phytase treatment and supplementation with different iron compounds on non-heme iron absorption in human from a mineral-supplemented oat-based beerage.

研究目的调查在添加矿物质的燕麦饮料中的不同铁化合物在经过添加柠檬酸、植酸酶处理以及铁剂补充后对人体非血红素铁吸收的影响。

Further studies with less cardiotoxic COX-2 inhibitors are needed in NSCLC.

采用较小心脏毒性的环氧化酶-2抑制剂更进一步的研究非小细胞肺癌治疗是必要的。

However, the molecular mechanisms underlying premature aging up 8-MOP/UVA are as yet unknown. In our studies, dermis of the non-lesion back skin of vulgar psoriatic patients who had been treated by PUVA and the cultured foetus dermic fibroblasts were selected to be observed.

本课题以PUVA治疗寻常型银屑病患者背部非皮损区真皮及培养的胎儿真皮成纤维细胞为对象，采用HE染色、Verhoeff染色、电镜、酶组织化学、免疫组织化学、原位杂交、流式细胞计数仪、三引物PCR、PCR-SSCP技术及PCR产物直接测序等技术和方法，研究了8-MOP/UVA对皮肤光老化形成的影响及芳维A酸乙酯的拮抗作用。

Laccases are able to oxidise both phenolic and nonphenolic compounds. They have broad ranges of subsrate specificity. Therefore, they are very useful for applications on pulp bleaching, textile dye decolouration, effluent detoxification, bioremediation and biosensors.

由于漆酶能够氧化芳香类化合物和其它一些非芳香类有机物，具有广泛的底物特异性，因此在纸浆漂白、纺织品染料脱色、有毒废弃物的去除、生物修复和生物传感器等方面具有巨大的应用潜力。

Methods Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用套式聚合酶链反应方法对5例艾滋病患者血浆HIV-1 env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及核苷酸序列进行分析，并计算其核苷酸同源替换率与非同源替换率的比值。

The proportion of GGT complexed with low density lipoproteins plus very-low density lipoproteins in total GGT in serum is determinated with ratio colorimetery,sodium phosphotungstate and MgCl2 as precipitation.26 patients with liver cancer,18 with liver cirrhosis,22 with chronic hepatitis,14 with the other malignant tumors,16 with non-hepatobiliary disease of high GGT and 50 healthy subjects have been evaluated.

本文以磷钨酸－氯化镁作沉淀剂，速率法测定血清低密度脂蛋白，极低密度脂蛋白结合的r－谷氨酰转移酶（LDL－VLDL－GGT）占总GGT的百分比。对临床26例肝癌、18例肝硬化、22例慢性肝炎、14例其他恶性肿瘤、16例GGT升高的非肝脏疾病及50例健康人进行了检测，结果表明：肝癌组明显高于其他各组，差异显著（P＜0.01）。肝脏疾病升高幅度：肝癌＞肝硬化＞慢性肝炎，且每两者间有显著差异（P＜0.01）。

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

The study performed by the New England Journal of Medicine suspects that the risk of death is increased by nearly 50 percent in patients who are administered Trasylol instead of alternative mediations.

这项研究由美国的新英格兰医学杂志嫌疑人，死亡的危险增加了近50%的病人抑肽酶经管而非替代斡旋。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。