非酶的

Glucose-6-phosphate dehydrogenase (G6PD, E. C. 1. 1. 1. 49) is a house-keeping enzyme in all cells, and also is a key enzyme in the pentose phosphate pathway. G6PD deficiency, affecting 200～400 million people in the world, can cause chronic non-spherocytic hemolytic anemia, favism, drug-induced hemolytic anemia, neonatal jaundice and several infectious diseases. Its inheritance mode is an X-linked incomplete dominance.

葡萄糖-6-磷酸脱氢酶（Glucose-6-Phosphate Dehydrogenase，G6PD，E.C.1.1.1.49）是存在于所有细胞中的一种看家酶，也是磷酸戊糖途径的关键酶。G6PD缺乏症累及全球2～4亿人，引起慢性非球形细胞溶血性贫血、蚕豆病、药物性溶血、新生儿黄疸及某些感染性溶血，其遗传方式是X连锁不完全显性方式。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题，采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

Another method that has been proposed to identify S.aureus from nonclinical sources employs an immunoenzymatic assay using a monoclonal antibody assay using a monoclonal antibody prepared against S.aureus endo-β-acetyl-glucosaminidase, an enzyme produced by all isolates of this species.

另一种方法，提出了确定非临床源金黄色葡萄球菌从员工的免疫酶检测用单克隆抗体检测用单克隆抗体制备对金黄色葡萄球菌内切β-乙酰氨基葡萄糖苷酶，这种酶产生的所有分离这一物种。

A nonproteinaceous organic substance that usually contains a vitamin or mineral and combines with a specific protein, the apoenzyme, to form an active enzyme system.

辅酶非蛋白质有机物质，通常含有维他命或矿物质，结合特定的蛋白质和酶朊，形成活性酶系统

A nonproteinaceous organic that usually contains a vitamin or mineral and combines with a specific protein , the apoenzyme , to form an active enzyme system .

辅酶非有机，通常含有维他命或矿物质，结合特定的和酶朊，形成活性酶系统

A nonproteinaceous organic substance that usually contains a vitamin or mineral and combines with a protein , the apoenzyme , to form an active system .

辅酶非蛋白质有机物质，通常含有维他命或矿物质，结合特定的蛋白质和酶朊，形成活性酶系统来源：词友

A nonproteinaceous organic substance that usually contains a vitamin or mineral and combines with a specific protein , the apoenzyme , to form an active enzyme .

辅酶非有机物质，通常含有维他命或矿物质，结合特定的和酶朊，形成活性酶

After inoculation of leukemia virus,the changes in ANAE,AcP and β-Gase reactions were observed in the thymocyte and thymie nurse cell in thymus cortex of experimental mice.

对接种白血病病毒后30d、75d 小鼠胸腺和淋巴结的组织结构及酸性非特异性酯酶、酸性磷酸酶、β～葡糖苷酸酶反应类型进行了观察，并与正常组作对照。

Carbonyl reductase is a monomeric oxidoreductase that catalyzes the NADPHdependant reduction of a variety of quinones, ketones, and aldehydes. It is widely distributed in the cytoplasm of various tissues of mammalian and non-mammalian species, and is involved in detoxification, physical regulation, as well as in drug metabolism.

羰基还原酶是一类依赖于辅酶NADPH催化各种醌、酮和醛等物质还原的单体氧化还原酶，它们广泛分布于哺乳动物和非哺乳动物各种组织的细胞质中，具有解毒、生理调节和药物代谢等重要功能。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。