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Methods ES cells of BALB/C mice were cultured in directional differentiation and non directional differentiation culture system respectively.

方法选用BALB/c小鼠ES细胞,分别进行未分化培养和非定向分化培养,对培养系统中ES细胞的发育和分化情况进行观察。

In addition, human recombinant K1-5 potently inhibited endothelial cell proliferation with no inhibition on non-endothelial cells.

MTT实验结果显示,重组人K1-5特异地抑制内皮细胞的增殖,而对非内皮细胞无抑制作用。

As a main subpopulation of CTL, CD8+CTL can eliminate viral through cytotoxic mechanism and non-cytotoxic mechanism.

CD8+CTL是CTL的主要功能亚群,他们通过细胞毒机制和非细胞毒机制清除病毒。

Although, in our study the functional characteristics of these T cells was not investigated, we can speculate that in HBsAg inactive carriers, a dynamic population of HBV specific T cells, both in the liver and periphery exists, that is able to produce antiviral cytokines (i.e. IFN-gama) and thus suppress HBV replication without cytolytic liver damage.

我们虽然没有研究这些细胞的功能特征,但是可以推测:非活动性乙肝病人机体内,有一个具有动态数量的HBV特异性T细胞群,在肝组织和外周都存在,它们能够产生抗病毒因子抑制病毒的复制,同时不造成肝细胞损伤。

ECP 63 might be a useful molecular marker for identifying embryogenic cells and non-embryogenic cells.

ECP63 cDNA可作为识别胡萝卜胚性细胞和非胚性细胞的一个分子标记。

Western Blot indicated that AIF was selectively released before cytochrome C from the intermembrane space of mitochondria to cytosol, and that release needed Bax relocation to mitochondria. Results above suggest that caspase-independent cell death maybe occurred earlier than caspase-dependent cell death in staurosporine induced T lymphocytes apoptosis. Caspase-independent cell death is an alternative pathway of cell death which could guarantee the safe and noninflammatory removal of corpses when caspase was mutented or blocked in the cell, it plays an important role in maintaining the number of T lymphocytes and ensuring the proper function of immune system.

细胞caspase非依赖死亡方式可能是Caspase依赖性死亡方式的替代途径,它可以保证在Caspase发生突变或通路受阻时,T细胞仍然能够正常死亡,这对于维持T细胞的数量和保证免疫系统正常功能的发挥具有十分重要的意义。

Considering undividing cells might be transfected with adenovirus vector andmacrophages could be activated by IL-2,we transfected mIL-2 gene into the freshlyisolated peritoneal macrophages with recombinant adenovirus,and pulsed with tumorantigen in vitro,in order to enhance the immune effector function as well as theantigen presentation function simutaneously,and acquired more effective actions ofantitumor.

本研究选用可转染非分裂细胞的缺陷型腺病毒为载体,将活化巨噬细胞的小鼠IL-2基因转染至小鼠腹腔巨噬细胞,并在体外行肿瘤抗原冲击,以期在增强其免疫效应功能的同时充分发挥其抗原提呈功能,从而取得更好的抗瘤效果。

Results Human complement can be activated by unsensitized EL-4 cell through the first pathway.

结果 未致敏EL-4细胞可直接以Ca2+、Mg2+离子依赖的第一途径活化人血清补体系统,在细胞数为1×104~2×104时,1:200稀释的人血清补体为EL-4细胞的非致死剂量。

Methods genomic dna was extracted from different stages of malignant transformation and tumorigenicity in inoculated athymic mouse 16hbe cells induced by cadmium chloride and methylating status of genome p16 gene promoter was observed with methylation-specific pcr and then compared with the controls.

从各组cdcl2恶性转化不同阶段及接种裸鼠成瘤的16hbe细胞中提取全基因组dna,采取甲基化特异性pcr法(methylation-specific pcr,msp)检测该基因组p16基因启动子区的甲基化状况,与非转化的16hbe对照细胞进行比较,并用去甲基化因子5-azac (5-aza-2'deoxycytidine)处理有异常甲基化的细胞。

DNA fluorescence dye Hoechest 33342 was used tomonitor the condensation of chromatin during germinal vesicle breakdown.

采用直接取核法获取卵母细胞生发泡,用同步化的HeLa细胞裂解液构建非细胞体系,将游离的生发泡培养在裂解液中,并加入荧光染料Hoechest 33342,观察染色质的变化情况。

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