非细胞的

The cells were preincubated at first in liquid phase for 24 hours at a concentration of 5×106 nucleated cells/ml of RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and concanavalin A5μg/ml of culture medium.

先将脾脏有核细胞(5x10~6/ml)在含有ConA(5μg/ml)的液相中培养24小时，然后收集非粘附细胞，将其接种到含0.88％甲基纤维素、10％FCS，ConA 5μg/ml和15％ConA-LCM的半固体系统中培养，细胞的终浓度为5x10~5/ml。

Objective: To study the growth inhibition and radiosensitivity effect of Gemcitabine on non-small cell lung cancer cells.

目的：研究吉西他滨对体外培养非小细胞肺癌细胞的细胞周期改变和凋亡影响，及其放疗增敏的作用及机制。

FN and LN (the non-collagenous index) out of ECM were measured by immuno-histochemistry mean, and the accumulation of ECM was measured by typeⅠand Ⅲ collagen ,α-SMA was chosen as a symbol of the activated reposit fat cell. TGF-β〓 was chosen as a symbol of the cellular factor made the reposit fat cell activated. We observed the mechanism of 286 through the variety of the upper indexes during the experiment.

用免疫组化方法测评肝细胞外基质中的非胶原指标FN、LN，胶原指标Ⅰ、Ⅲ型胶原等在实验过程中的变化作为判定ECM沉积增多的事实指标；用a-平滑肌肌动蛋白作为贮脂细胞被激活的标志物指标；用转化生长因子-β〓作为激活贮脂细胞的细胞因子指标；通过观测上述指标在实验中的变化，来探讨"268方"的作用机理。

FN and LN(the non-collagenous index) out of ECM were measured by immuno-histochemistry mean, and the accumulation of ECM was measured by type I amd III coHagen, a -SMA was chosen as a symbol ef .the activated reposit fat cell.TGF- P1 was chosen as a symbol of th ieccellular factor made the reposit fat cell activated.We observed the mi sclnanism of 286 through the variety of the upper indexes during the experiment.

用免疫组化方法测评肝细胞外基质中的非胶原指标FN、LN，胶原指标Ⅰ、Ⅲ型胶原等在实验过程中的变化作为判定ECM沉积增多的事实指标；用a-平滑肌肌动蛋白作为贮脂细胞被激活的标志物指标；用转化生长因子-β_1(TGF-β_1)作为激活贮脂细胞的细胞因子指标；通过观测上述指标在实验中的变化，来探讨"268方"的作用机理。

The results showed that SiO2 and total protein contents, PAM numbers and LDH activity in silicotic rats decreased gradually with the severity of silicotic pathologic changes.

结果表明，矽肺大鼠BALF中SiO2粉尘和总蛋白量以及细胞总数和LDH活性，均随矽肺病变加重而逐渐减少。虽然，BAL对不同程度矽肺病变均能洗出一定量的细胞和非细胞成分的物质，但以早期病变组排出量最多。

NF-κB is a kind of important nuclear factors which are relative to lots of cellular activities such as activation of immunocyte, development of T and B lymphocyte, stress reaction, cell apoptosis and so on. NF-κB exists in almost all types of cytoplasm in the unreactive form of heterodimer or homodimer.

核转录因子κB(nuclear transcription factorκB， NF-κB)是一类关键性的核转录因子，通常以同源或异源二聚体非活性形式存在于几乎所有类型细胞的胞质，其与免疫细胞的活化，T，B淋巴细胞的发育，应激性反应，细胞凋亡等多种细胞活动有关。

A portion of ductal cells and individual serous acinic cells of the minor salivary gland also expressed Fas not only in membranous but also in cytoplasmic pattern.

结果发现，在正常鼻咽粘膜表面及隐窝的假复层纤毛柱状上皮细胞胞膜、部分小涎腺的导管上皮和个别浆液性腺泡上皮细胞的胞膜和胞浆Fas阳性表达；鳞状化生上皮的表层细胞胞膜有Fas表达，但基底细胞未见Fas表达；浸润的淋巴细胞也不表达Fas.45.9%（17/37）的鼻咽非角化性癌Fas阳性，但只有不足10%的癌细胞胞膜微弱表达Fas。

We theoretically investigated the effects of incorporating Ih, T-type Ca2+ current, sustained inward current, and/or low-voltage-activated L-type Ca2+ channel current on 1 creation of BP cells, 2 robustness of BP activity to electrotonic loads of nonpacemaking cells, and 3 BP cell ability to drive NP cells.

我们从理论上研究了合并Ih， T-型 Ca2+流，持续内向电流，和/或低电压激活的L-型Ca2+通道电流对1产生 BP细胞， 2 BP 活性对非起搏细胞电紧张负荷的稳定性，和 3 BP 细胞驱动 NP细胞的能力的作用。

Part I: Primary culture and purification of skeletal muscle derived satellite cells for tissue engineering applications To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique for tissue engineering applications.

第一部分：种子细胞—肌卫星细胞的原代培养及鉴定本部分通过应用改良Percoll非连续密度梯度离心法分离纯化培养新西兰兔骨骼肌干细胞—肌卫星细胞，探讨为组织工程膀胱的构建提供种子细胞的可行性。

This review focuses on the current research progress of the liver cell differentiation source, and it also discusses the advantages and disadvantages of various hepatic stem cells and nonhepatic stem cells, expecting to provide a reference for liver cell source selection during tissue engineering, an adequate and suitable source for bioartificial liver and hepatocyte transplantation and to help to treat liver diseases.

本文着重论述了目前肝脏细胞的分化来源研究进展情况，并对各种肝源性和非肝源性干细胞的优缺点进行了讨论，以期为肝组织工程选择细胞来源提供参考，为生物人工肝、肝细胞移植提供充足、合适的细胞来源，协助治疗肝脏疾病。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷