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Methods: Study the newly found cell with the weak silver carbonate staining method of del Rio-Hortega. Using transmission electron microscopy, compare the newly found cell with the atypical cell found by Marc Lenoir and Philippe Vago, and extend young SD rat model to adult SD rat, young and adult Wistar rat,and extend drug of Neomycin to Amikacin to study the universality.

采用经典的小胶质细胞特殊染色方法——银染法(Hortega氏碳酸银法)对大鼠耳蜗药物损伤后出现的新细胞进行研究;通过透射电镜与Marc Lenoir和Philippe Vago实验动物模型中的"非典型细胞"进行了比较与扩展研究;运用逆转录PCR技术检测耳蜗基底膜上神经干细胞标志物nestin的表达情况,对新细胞的来源及其与神经干细胞或nestin的前体细胞的关系作进一步研究。

On the functional differentiation,we found and that the type II ras-GTPaseactivating protein (p100-GAP),which was specifically expressed in humanplacenta,was largely expressed in the highly-differentiated syncytiotrophoblastcells and moderately expressed in the cultured intermediate trophoblast cells.While in the non-differentiated cytotrophoblast cell line-NPC,no expression was observed.Its mRNA and protein expressingcharacterization was in consistent with that of hCGβ,which was one of the mostimportant markers of the trophoblast cell functional differentiation.In addition,the expressing amount of p100-GAP increased with the progressing of pregnancyand the syncytium formation in vitro.

细胞功能分化的研究结果表明:经重组质粒的构建获得特异表达于人胎盘的p100-GAP的cDNA探针后,从蛋白和分子水平证实p100-GAP大量表达于分化程度高的合体滋养层细胞,少量表达于体外培养的中间型滋养层细胞,而非分化的细胞滋养层细胞NPC则不表达,这种表达特性与作为滋养层细胞功能分化重要指标之一的hCGβ高度一致;p100-GAP表达量还随妊娠过程和体外合体化过程而逐渐增加。

Intracellular dialysis of rhyn did not accelerate the slow current decay, suggesting that this compound acts extracellularly.

当细胞内加入钩藤碱时,不会加快慢性钝化过程,所以我们推测钩藤碱作用在Kv通道细胞外的部分,而非细胞内。

At 10^(-9)mol/L of the immunotoxin, 3 logs (99.9%) of target cells were killed, but no cytotoxicity on nontarget cells.

在1×10^(-9)mol/L浓度时对靶细胞的杀伤可达到99.9%,而对非靶细胞则无细胞毒作用。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞,在含有25μg/L M-CSF和0,1,10,100μg/L TNF-α的α-MEM培养液中培养5d后,观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成;细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养,比较加入和不加入10μg/L TNF-α培养4、5、6和9d后,所形成的TRAP多核细胞的数目和骨吸收面积。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞,在含有25μg/LM-CSF和0,1,10,100μg/LTNF-α的α-MEM培养液中培养5d后,观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成;细胞在含有25μg/LM-CSF和30μg/LsRANKL的α-MEM培养液中进行培养,比较加入和不加入10μg/LTNF-α培养4、5、6和9d后,所形成的TRAP多核细胞的数目和骨吸收面积。

When cells undergo programmed cell death, cell corpses adopt a refractile and disc like structure. Using this morphological change as a cell death marker, we analyzed the death of the first 13 cells in the AB cell lineage of wild type and cnx-1; crt-1 mutant embryos.

进一步以4D摄影分析线虫AB世系细胞中最早死亡的13颗细胞之相对死亡时间和细胞尸体持续的时间长短的结果则显示,此死亡细胞数量提高的现象应是源於吞噬步骤发生问题而非细胞死亡执行上的问题。

Results 17β-HSD type 2 was found in the cytoplasm of tumor cells in 5.3% of cases.In adjacent non-malignant tissues,82.9% of cases were positive and the enzyme was detected in the cytoplasm of epithelial cells of the acini and ducts.

结果:2型17β-HSD在乳腺癌细胞的细胞浆中有表达,阳性率为5.3%;在癌旁非恶性乳腺组织腺泡上皮细胞以及导管上皮细胞的细胞浆中表达,阳性率为82.9%。

The number of BrdU labeling in the nucleus was remarked increasing onbFGF-treated pancreatitis rats than pancreatitis rats without bFGF treatment. And there was a significant difference.(P.01)ConclusionThe result of the study has demonstrated the effective therapeutic effects on acute edematous pancreatitis rats, which induces proliferation of immature acinar cells, facilitates the repair of damaged pancreatic tissues and reconstitutes tissue integrity.

兔疫组化结果显示bFGF治疗组大鼠具有BrdU标记的细胞核胰腺细胞数明显多于非治疗对照组大鼠,两者在统计学上具有显著性差异Oeq.of人结论实验结果表明bFGF对急性水肿性胰腺炎大鼠具有明显的治疗作用,它的治疗作用具体表现在诱导非成熟的胰腺细胞分化增殖,促进受损胰腺组织修复,重建组织完整性。

In Group B, the value of cell and acellular component were similar to those reported in references.

非感染病例的支气管肺泡灌洗液的细胞及非细胞成分的数据与文献报道的正常人数据相仿。

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