非细胞的

Methods: Tumor cell-platelet adhesion of highly metastatic SACC-LM, non-metastatic SACC-83 and effect of aspirin, arginine-aspartate , magnesium acetylsalicylate on adhesion were studied in vitro. Antimetastatic effect of aspirin, RD, magnesium acetysalicylate on experimental metastasis of SACC was observed in vivo.

体外实验观察高转移潜能的SACC-LM细胞、非高转移潜能的SACC-83细胞与血小板的粘附，阿斯匹林、阿斯匹林镁、精氨酸-天冬氨酸（arginina-aspartate，RD）对SACC-LM细胞与血小板粘附的影响；动物实验观察阿斯匹林、阿斯匹林镁、RD对SACC-LM细胞实验性肺转移的作用。

Struma ovarii, primary or secondary malignant melanoma and some sertoli cell tumor in which the cell distended by intracytoplasmic lipid were dominant.

非特异性类固醇细胞瘤需要与广泛黄素化的颗粒细胞瘤、卵泡膜细胞瘤、富于脂质的Sertoli细胞瘤和原发性或继发性的黑色素瘤相鉴别。

The in vitro effect of Astragalus radix water extract onthe non-specific immune responses of macrophages isolated from the head kidney of Cyprinus carpio was evaluated.

用RPMI细胞培养液对分离的细胞进行原代培养，在细胞培养液中加入不同浓度的黄芪水提取液来研究黄芪对鲤头肾中免疫细胞非特异性免疫应答的影响。

These nonmalignant cells, such as stromal fibroblasts and bone marrow-derived cells, have been shown to be capable of promoting tumor growth and metastasis.

这些非恶性细胞如基质成纤维细胞和骨髓源性细胞已被证明能够促进肿瘤的生长和转移。

The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division.

结果表明，非胚性愈伤组织转入到MS + 4.0 mg · L-1 2，4-D + 0.5 mg · L-1 6-BA培养基培养后，愈伤组织中先出现一些胚性细胞；然后单个胚性细胞分裂形成一个由多细胞组成的原胚。

Methods Human prostate cancer PC-3 cell, breast cancer ER-75-30 cell and lung cancer A549 cell were cultured in RPMI medium 1640.Light microscope,transmissional electron microscope, flow cytometer and immunohistochemical method were used to observe apoptosis morphological changes of three kinds of tumor cell lines, time and dose effect relationship and to detect expression of bcl-2 and bax.

方法人前列腺癌 PC-3细胞株、乳腺癌 ER-75-30细胞株和非小细胞肺癌 A549细胞株经培养后，采用光镜、电镜、流式细胞仪和免疫组化方法研究 188Re诱导 3种肿瘤细胞凋亡的形态学变化、时间剂量效应关系、细胞周期依赖性以及凋亡相关基因 bcl-2和 bax在其中的表达情况。

The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid.

取小鼠外周血非贴壁的淋巴细胞，与靶细胞混合淋巴细胞培养，进行淋巴细胞增殖和反应性T细胞功能检测，并检测细胞培养液中IL-2、TNF-α和IFN-γ水平。

Some exudation and bleedings happen in the pulmonary alveoli, some inflammatory cells infiltrate into lung tissues; in the serum AMY, ALP, TNF-α, IL-6 increases obviously high, COX-2 expression increase; The expression of NF-κB in big rat lung of SAP increase, and the nucleus is thick dyed, it explain that the NF-κ moved into the nucleus; Stimulate the inflamatory reaction.

我们的实验中见到SAP组的大鼠肺脏组织细胞腔扩大、肺泡壁断裂，有的出现肺大泡，肺泡内伴有渗出及少量非出血，肺组织可见咽细胞浸润；血清中AMY、ALP、TNF-α、IL-6明显增高；在SAP组大鼠肝细胞、肾小管上皮细胞、肺脏小气管及肺泡上皮上COX-2表达增加；NF-κB在SAP大鼠肺脏中表达增加，并且出现核浓染，说明NF-κB前移入核；启动炎症反应等一系列反应SAP大鼠受损伤的肺组织中AQP-1无论在mRNA水平上、蛋白水平上还是免疫组化染色病理学检测上都明显下降。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Department of Haemotology,Zhongda Hospital,Southeast University,Nanjing210009,China）Abstract:Cytokine induced killeris a new kind of immune cells which have non MHC restric-tion,highly proliferation and cytotoxicity.Their activities are superior to lymphokine activated killer cells.Immunotherapy of CIK cells can significantly improve clinical symptoms,but hardly have any side effects.

摘要］ 细胞因子诱导的杀伤细胞（cytokine induced killer，CIK）是一种非MHC限制性的新型免疫活性细胞，其增殖能力强、杀瘤活性高、副作用小，优于淋巴因子激活的杀伤细胞，对于提高患者自身抗肿瘤免疫能力有重要作用。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷