非细胞的

Skin suqamous cell carcinoma, a kind of skin tumor origined fromkeratinocyte or skin appendant organ suqmaous cell, is one of the most commonnon-melanoma skin cancers (about 20%).

咪喹莫特对人皮肤鳞状细胞癌SCL-1细胞株作用机制的研究前言皮肤鳞状细胞癌是起源于角质形成细胞或附属器鳞状上皮细胞的恶性肿瘤，是最常见的皮肤恶性肿瘤之一，其发病率约占全部非黑素性皮肤细胞癌的20％。

The mechanisms of apoptosis of skin squamous cell carcinoma induced by IFN-γand ATRA Introduction and objective Skin squamous cell carcinoma is a malignant neoplasm derived from epidermal keratinocyte and squamous cells of skin appendant organ .It is one of the most common skin carcinoma, and the incidence rate of SCC is 20% in malignant non-melanoma. It is a common disease that seriously harms health of people in our country, so we should pay more attention to it.

维甲酸、γ-干扰素诱导皮肤鳞状细胞癌SCL-1细胞凋亡的机制研究前言与目的皮肤鳞状细胞癌(squamous cell carcinoma，SCC，简称鳞癌)是起源于表皮角质形成细胞或附属器鳞状上皮细胞的一种恶性肿瘤，是常见的皮肤恶性肿瘤之一，其发病率约占全部非黑素细胞癌的20％。

The mechanisms of apoptosis of skin squamous cell carcinoma induced by IFN-γand ATRA Introduction and objective Skin squamous cell carcinoma is a malignant neoplasm derived from epidermal keratinocyte and squamous cells of skin appendant organ .

维甲酸、γ-干扰素诱导皮肤鳞状细胞癌 SCL-1细胞凋亡的机制研究前言与目的皮肤鳞状细胞癌(squamous cell carcinoma，SCC，简称鳞癌)是起源于表皮角质形成细胞或附属器鳞状上皮细胞的一种恶性肿瘤，是常见的皮肤恶性肿瘤之一，其发病率约占全部非黑素细胞癌的20％。

Methods Proliferation,cytotoxicity in vitro and expression of CD11 c of the cells cultured in the two different media were compared.To observe the tumor target cells killed by adˉherent natural killer cellsthrough electroscope.

分别用AIMV及完全培养基培养粘附NK细胞和非粘附NK细胞，比较细胞的增殖能力、杀伤肿瘤细胞的能力、表达粘附分子CD11 c 的能力，并通过电镜对A-NK细胞所杀伤的肿瘤细胞进行形态学观察。

Ore-binding factor alpha 1 plays an essential role in bone formation and osteoblast differentiation and serves as a master gene regulating osteoblast-specific gene expression. The gene is expressed in cells of the osteoblast lineage only, and when expressed in non-skeletal cells, the cells assume many of the characteristics of an osteoblast.

bfal在骨形成和成骨细胞分化过程中起着重要的作用，是调控成骨细胞特异性基因表达的主控基因，该基因只在骨形成相关细胞中表达，如果使其在非成骨细胞中表达，该细胞将出现成骨细胞的特征。

Fibroblasts have the ability to secret many kinds of extracellular matrix and cytokines relevant to hepatogenic differentiation. So, we investigate whether coculture of hADSCs with fibroblasts without cell-cell contact play a role in hepatic differentiation of hADSCs by RT-PCR analysis of hepatic specific gene expression and real-time PCR analysis of Alb expression.

成纤维细胞能够分泌很多利于干细胞向肝细胞分化的细胞外基质和细胞因子，我们采用非接触共培养的方式对成纤维细胞与人脂肪干细胞共培养能否引发其向肝细胞的分化进行了研究。

In order to distinguish the type of GFP positive cells in glomeruli further, we also carried out fluorescence histochemistry double staining, the results of it were: the GFP positive cells in recipients glomeruli in mice of the four, five, six groups were blood cells in glomeruli capillary loop, but not the glomeruli resident cells, for example, endothelial cells, epithelial cells or intercapillary cells.

为了进一步鉴别受体鼠肾小球内GFP阳性细胞的种类，我们进行了免疫荧光组织化学双染色，结果显示：④、⑤、⑥组受体鼠肾小球内的GFP阳性细胞可能是肾小球毛细血管袢内的血细胞而非肾小球的固有细胞，即内皮细胞、上皮细胞或系膜细胞。

The frequency value in S phase is 4. 83 times of M phase, 2. 9 times of G1 phase, and 3. 41 times of unsynchronized cells. These results suggested that gene correction events were highly related to S phase. Then we compared the targeting efficacy for two groups of cells in S phase. One group was contact with thymidine and the other one went smoothly through S phase without replication blockage.

基于这种原因，我们对基因定点修复事件做了细胞周期定位分析，发现同步于S期的细胞群发生定点修复的效率明显高于其它各期以及非同步化的细胞，流式细胞仪检测S期定点修复效率为M期细胞的4.83倍，为G1期细胞的2.9倍，是未同步化细胞的3.41倍。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Objective: Comparative and extended research among the newly found cell, microglia cell and atypical cell in the rat organ of Corti after aminoglycoside intoxication; to further explore quality, origin, and the relationship between the newly found cell and the neural stem cell or nestin progenitor cell.

目的：对大鼠耳蜗药物损伤后出现的新细胞与小胶质细胞、非典型细胞进行比较和扩展研究，探讨新细胞的代表性及其起源、转归、以及与神经干细胞或nestin前体细胞的关系。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷