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Both specific and non-specific host defence mechanisms are responsible for the protection of fish against challenge infections with this ciliate.

鱼类的特异性和非特异性宿主防御机制都负责保护鱼类抗小瓜虫感染。

objective to study the anti-tumor activity and enhancing immune effect of phellinus linteus.methods the anti-tumor activity of phellinus linteus was estimated by the longevity of eac mice,the immune effect of tumorous mice was tested by the experiment of carbon particle expurgation and the il-2 concentration in serum of tumorous mice was determined by elisa.results phellinus linteus can prolong eac mouse's life and can increase the il-2 concentration in serum of tumorous mouse but can't affect the immune effect of tumorous mouse.conclusion chinese hurb phellinus linteus possess some antitumor power for tumor-loaded mice,but further research is needed.

目的 观察桑黄对肿瘤的抑制作用及对机体免疫功能的影响。方法以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用,以炭末廓清实验观察荷瘤小鼠非特异性免疫功能,以elisa法测定荷瘤小鼠血清il-2浓度。结果桑黄对eac荷瘤小鼠的生存时间有延长作用,对荷瘤小鼠血清il-2浓度有提高作用,对荷瘤小鼠非特异性免疫功能无明显影响。结论中药桑黄对荷瘤小鼠具有一定的抗肿瘤能力,作用的强度和作用机制的深度还有待进一步研究。

In addition, the formation of specific immunotherapy, in turn enhance the body of the non-specific immunity.

此外,特异性免疫的形成过程,又反过来增强了机体的非特异性免疫。

The Nature of Antibodies and the Immune Response in Rainbow Trout.

抗体是由抗原免疫后产生的,与某一抗原专一性地结合,特异性程度高;而凝集素是天然存在的,不需经过免疫而产生,能与多种抗原发生凝集反应,具有非特异性。

Nonspecific cytotoxic cell is the evolutionary precursor of mammalian natural killer cell in teleostean fish, which was mainly separated from blood and lymphoid organ as the first barrier of defense to protect the animal against bacteria, virus and protozoa parasitism, it plays an important role in nonspecific immunity and also has a function of killing tumor cells in vitro.

自然杀伤细胞进化上的前体细胞在鱼类中被称作非特异性细胞毒性细胞,主要来源于血液和淋巴器官,是防御细菌、病毒、寄生性原生动物等入侵的第一道防线,在非特异性免疫中起重要作用,还具有体外杀伤肿瘤细胞的功能。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC;分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态;流式细胞术检测DC表型;HRP吞噬实验测定DC的群体内吞能力;MTT法检测同种异体混合淋巴细胞反应;ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平;冻融法制备肝癌细胞可溶性抗原;流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性;MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响;SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度,ELISA测定活性;流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型;MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应;流式细胞术检测肝癌细胞表面HLA-DR表达;MTT法检测肝癌DC疫苗对自身淋巴细胞的活化;原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达;流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L;MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用;Cell-ELISA检测人肝癌细胞hAFP表达;MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响;ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平;肝癌细胞凋亡的诱导和检测;DC吞噬凋亡肝癌细胞后的电子显微镜观察;DC对肝癌细胞的生长抑制试验;人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定;DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤;冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

It is concluded that both inasie and noninasie pulmonary function tests are capable of detecting allergen-specific as well as non-allergic bronchoconstriction in intact mice or rats.

结论是:无论是侵入性还是非侵入性肺功能实验,在大鼠或小鼠体内,都能检测出特异性变应原和非特异性变应原引起的支气管收缩。

In conclusion, the non-specific immunity of Portunus trituberculatus was significantly affected by salinity low 14 and high 36 stress during 12 hours; PSVF2 has more immunity advantages in these non-specific immune factors than that of CPF1 under salinity stress.

总结:低盐和高盐胁迫对三疣梭子蟹的非特异性免疫影响显著且表现出时间效应;在正常和抗盐度胁迫下,筛选蟹F2比普通蟹F1在非特异性免疫因子方面更具免疫优势。

The sensitivity, specificity and Youden's index of PCR were 88.9%, 90.9% and 0.798 respectively when blood culture and five non-specific tests were used as a reference method.

若以血培养加非特异性指标为诊断标准,PCR法的灵敏度为88.9%,特异性为90.9%,正确诊断指数达到0.798。

This paper dealed with the immunnodulation tests of white wax scale in specific and unspecific immunity. The result showed that the IgM in high dose group was clearly higher than that of the control in serolysin test. Lymphocyte transformation and propagation in mice were notable in high dose group.

对白蜡虫的特异性和非特异性免疫调节功能进行了试验研究,结果表明:特异性免疫的血清溶血素试验中,白蜡虫高剂量组小鼠的溶血素生成量明显高于对照,淋巴细胞转化增殖试验中,白蜡虫高剂量组小鼠的淋巴细胞转化增殖明显。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。