非特异性的

Sensitive range from 49.1% to 100%, specificity range from 18.2% to 90%,accurate range from 45.5% to 88.3%, PPV range from 34.1% to 95.2,NPV range from 22.2% to 100%,the combine sensitive was 82.7%,specificity was 63.3%;there were not obvious publish bias in the 9 articles, and there was heterology of the 9 articles, the forest figure show RR was 2.34[1.43,3.05],and its 95% confidence interval did not include 1,the chance of malignancy nodules'SUV above 2.5 were more than benign nodules.

文献报道的敏感性49.1-100%，特异性18.2-90.0%，准确性45.5-88.3%，阳性预测值34.1-95.2%，阴性预测值22.2-100%，Meta分析合并后的敏感性82.7%，特异性为63.3%，准确性为71.8%，阳性预测值80.8%，阴性预测值57.0%；漏斗图成近似倒三角形，认为文献无明显发表偏移；对文献异质性检验，P小于0.05，认为文献来源存在异质性；选用随机效应模式得到森林图，RR为2.34[1.43，3.05]，其95%的可信区间不包括1，认为肿瘤病人最大SUV 2.5法呈阳性的可能性大于非肿瘤病人，二者之间具有统计学差异；两篇来源于日本的文献显示日本国的18F-FDG PET肺占位显像假阴性率高于我国。

Methods The differentially expressed genes between liver tumor and non-tumor tissue were cloned by using suppression subtractive hybridization. Differentially expressed genes were sequenced and analized for homology in Gene Bank. In situ hybridization was performed to identify special expression of differentially expressed genes in livre tumor and non-tumor tissues.

方法通过抑制消减杂交法克隆土拨鼠HBV引发的肝癌组织和非癌组织中差异表达的基因；对差异表达基因进行DNA序列测定后于Gene Bank内分析其同源性；应用原位杂交确定差异基因在肝癌组织和非癌组织中的特异性表达。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

Acephala By using SCR specific primers based on the NH_2-terminal region of SCR proteins and anchored oligo_(18) primer, SCR cDNA fragment was isolated from Brassica oleracea var.

然后，利用甘蓝SCR_(13)的5'非翻译区和3'非翻译区设计SCR_(13b)的特异性引物，获得了羽衣甘蓝SCR_(13b)编码区序列。

Results (1)CIMT and FIMT in CHD group were significantly higer than those in non-CHD group(p＜0.05), The detection rate of plaques in CHD group was significantly higer than those in non-CHD group(p＜0.05);(2) The sensitivity of carotid and femoral atherosclerosis plaques in young CHD patients was 62% and 57%. The specificity was 90% and 86%. The positive predictive value of carotid and femoral atherosclerosis plaques in young CHD patients was 92% and 88%. The negative predictive value was 58% and 53%. The predictive value of combinant measurement of carotid and femoral atherosclerosis plaques was not improved.

结果 （1）冠心病组CIMT、FIMT与非冠心病组比较，前者均高于后者（p＜0.05）；冠心病组颈动脉、股动脉粥样硬化斑块检出率均明显高于非冠心病组（p＜0.05）；（2）颈动脉、股动脉粥样硬化斑块预测年轻冠心病的敏感性分别为62%、57%，特异性为90%、86%，阳性预测值分别为92%、88%，阴性预测值为58%、53%；颈动脉、股动脉联合检测未见明显提高预测值。

Therefore the nonrandom distribution of 3′ end sequences of effectual primers in tomato RAPD reflected the sequence-specific structure may occur in tomato genome at flank of polymorphic regions. It is probably an attribute of tomato genomic sequence and is common in Lycopersicon.

RAPD引物3′端序列的非随机分布，反映了番茄基因组内的多态性区段侧翼具有序列的特异性，这种特异性结构应是番茄基因组序列的一种属性，这种属性为番茄属基因组所共有。

Then transplantation of the sciatic nerve from donor rats to the recipient rats in two groups were progressed.7 days after nerve transplantation,the contents of cd4 and cd8 in peripheral plasma of recipient rats were detected by flow cytometry.8 weeks after nerve transplantation,transplanted nerve were observed under light microscope.results:compared with nonirradiated group,the percentage of cd4 and the ratio of cd4 to cd8 in uvb irradiated group were significantly decreased（p＜0.05,p＜0.01）,cd8 percentage was significantly increased（p＜0.01）.conclusion:uvb-irradiated donor splenocytes were injected in the recipient rats through caudal veil,which could induce immunological tolerance to allogeneic transplantation.

未照射组注射未经紫外线照射的供体脾细胞，照射组注射经紫外线照射的脾细胞，7 d后移植供体神经。术后7 d行外周血t淋巴细胞亚群检查，术后8 w行移植神经组织学检查。结果：照射组cd4+百分率、cd4+/cd8+比值较非照射组明显降低（p.01，p.05），cd8+百分率较非照射组明显升高（p.01）。组织学检查显示，照射组的再生神经较非照射组多。结论：尾静脉内注射经紫外线照射的异基因脾细胞可诱导对异体神经移植的特异性免疫耐受。紫外线；脾细胞；免疫耐受；坐骨神经

All 31 YSTs (5 pediatric and 26 postpubertal) showed strong positive SALL4 staining in more than 90% tumor cells but had negative OCT4 staining. Both spermatocytic seminomas showed positive SALL4 staining in 80% to 95% tumor cells in all 3 types of tumor cells with weak-to-moderate staining intensity.

为了检验SALL4的特异性，我们还给23例睾丸的非GCTs（10例间质细胞瘤、4例支持细胞瘤、3例腺瘤样瘤、3例睾丸旁横纹肌肉瘤、2例弥漫性大B细胞瘤、1例睾丸网的乳头状囊腺瘤）和275例非睾丸性肿瘤（158例转移癌、12例转移性黑色素瘤、11例原发性和2例转移性间皮瘤、72例原发性和20例转移性肉瘤）做了SALL4免疫染色。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

EMB-resistance in 18 isolates was caused by the mutation at coden 306 in embB gene with positive rate of 62.1%. The PCR result was negative in 20 specimen from pulmonary diseases without active TB and 5 negative control strains of no MTB. The data in this study showed high specificity of 100%. Only one specimen in 7 specimen with negative result in acid-fast stain and cultivation was shown alteration in coden 306 in embB gene.

结果：130例活动性肺结核患者痰标本抗酸染色阳性38例，阳性率为29.2%(38/130)；罗氏培养阳性为79例，阳性率为60.8%(79/130)。79例培养阳性痰标本中耐乙胺丁醇29例，其中有18例在embB基因met306ATG突变，基因突变率为62.1%。20例非结核肺部疾病患者痰标本和5株非结核分枝杆菌菌株经套式PCR扩增全部为阴性，特异性为100%。7例涂阴培阴PCR扩增阳性的序列中有1例在embB基因306位密码了发生突变。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。