非水平的

The result indicated that the percentage of lymphocyte were decreased, the percentage of neutrophile granulocytes were magnified significantly with the lighting time increasing, which was due to the level of the melatonin inhibited in the long time lighting; and to supply different exogenous dose, induce the percentage of lymphocyte were magnifying, the percentage of neutrophile granulocytes were decreased. And the statistical analysis showed there was significant difference.

结果表明：在给予不同光照时间的条件下，随着光照时间的延长，褪黑素的分泌水平受到抑制，导致具有特异免疫功能的淋巴细胞百分率下降，具有非特异免疫功能的中性粒细胞百分率上升，经统计分析表明不同组间的测定结果均存在显着差异；采用24h全天光照，用以模拟机体切除松果腺的情况，再给予一定剂量外源性褪黑素，导致机体内具有特异免疫功能的淋巴细胞百分率上升，具有非特异免疫功能的中性粒细胞百分率下降，经统计分析表明不同组间的测定结果均存在显著差异，并且高剂量褪黑素对机体的影响更为明显。

The result shows that: with the expansion of teacher education space, the reform and development of Chinese teacher education is much closer or equal to that of the international teacher education. The transitional stage and education reform in China puts forward a new demand for P. E. talents. The innovation as to education object, specification and curriculum arrangement raised in P. E. Curriculum Arrangement for Chinese Universities with four-year Program published in 2003 meets such a demand, and narrows the gap of education development in high P. E. academy between China and foreign countries. P. E. teachers abroad are usually cultivated by comprehensive universities, the cultivating objectives are verified, and generalists adapted to social development are emphasized. Cultivating mode abroad are various, with "open-type" or "non-directional type" as the main mode, and 4-year program, 5-year program, and 3-year program serve as the main form of education system. Curriculum arrangement abroad stresses general education, with foundationalization, normalization, objectification, optionalization, miniaturization, internationalization and syntheticalization as its characteristics and it employs credit system. The close-typed cultivating mode of P. E. academies in China should be gradually transformed into open-type or multi-type, cultivating generalists instead of specialists, and further constructing a new one with several cultivating types co-existing. Orientation of P.E. teachers in China should follow standard principle, coordination principle, experience principle and resource principle. To improve P.E teacher education in China, the following measures can be taken: relevant laws and regulations should be made for orientation; P.E teachers" training organization should be encouraged to participate in orientation; extension and connotation of P.E. teacher education should be expanded; resources should be optimize; systematic P.E. teachers" orientations should be organized;"Life P.E. Teacher Qualification "should be changed; useful-life should be set for P. E. teacher qualification; P.E. teacher qualification should be regularly authenticated; On-job training of P. E. teachers abroad tends to be systematic, but in China P.E. teachers" on-job training and prevocational training are separated; therefore measures must be taken to change this circumstance and thus speed up the systematic process. Factors concerning constructing a new system of P. E teachers are the following: reconstructing resources, making P.E. teachers" cultivation, orientation and training systematic, establishing a mechanism for encouragement and punishment, making school-based training and college training systematic, and setting up a mechanism combining directional training with non-directional training.

研究结果表明：随着教师教育革新空间的拓展，我国体育教师教育的改革与发展水平逐步与国际体育教师教育发展趋于接轨化。2003年颁布的《全国普通高校体育教育本科专业课程方案》在培养目标和规格、课程设置上的创新性，适应了我国社会转型和教育改革对体育教育专业人才的需求，进一步缩小了与国外高等体育院系教育发展的差距；国外体育教师一般由综合性大学或文理多科性大学培养，培养目标多向化，以培养适应社会发展的通才型人才为主；培养模式灵活多样，多数以"开放型"或"非定向型"培养模式为主，学制多以4年制、5年制、3年为主；课程设置上注重通识教育，突出基础化、师范化、针对化、选修化、小型化、国际化和综合化特点，实行完全学分制的教育管理制度；我国普通高校体育教育本科专业人才培养模式应该由封闭性转向开放式混合型的人才培养模式转变，由专才型转向复合型人才培养模式转变，构建多种类型并存的人才培养模式；我国体育教师入职教育革新应遵循的原则是本位原则、协同原则、经验原则和时间与资源原则，改进措施有：做好入职教育的法规化、制度化建设，激励体育师资培育机构参与体育教师的入职教育，拓展体育教师教育的外延和内涵，优化整合资源，系统组织体育教师的入职教育，改变体育教师资格"终身制"，设定体育教师资格有效期限，推行教师资格定期认证制度等；国际上体育教师在职培训体系显现了体育教师教育一体化的发展取向，但我国体育教师的在职培训与职前培养存在分离性，必须采取改革措施，加速我国体育教师教育的一体化进程；构建体育教师教育新体系的实施要素是：重组资源配置，实现体育教师培养、入职和培训一体化，建立奖惩机制，实现校本与院校培训一体化，建立体育教师定向与非定向培养培训有机结合机制。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

We also showed that coadministration of AG825 or AG1478 will enhance the efficacy of cytotoxic AGent cisplatin.

了解5种人类非小细胞肺癌细胞系A549、H322、H358、H460、H1299的HER2和EGFR蛋白表达水平，探讨5种细胞系HER2蛋白表达水平与顺铂IC50之间的关系；观察HER2和EGFR小分子酪氨酸激酶抑制剂AG825和AG1478对5种细胞系的生长抑制作用；观察AG825、AG1478联合顺铂对5种细胞系的协同生长抑制作用；研究AG825、AG1478联合顺铂对H322、H1299细胞系细胞增殖、细胞周期、细胞凋亡和坏死的影响；研究AG825、AG1478联合顺铂所致H322、H1299细胞系EGFR、磷酸化ERK和总ERK表达的变化，探讨协同生长抑制作用的机制。

The research displayed that yeasts Genus level can be discerned by agarose gel electrophoresis and polyacarylamide gel electrophoresis, but the latter is more advanced. The Saccharomyces cerevisiae strains ITS-1 sequences are most disalike, few of them are equal completely. The bigger differences are part of base pairs about Poly-T, others are exchanged by single base pair.

研究表明，以酵母菌株的rDNA ITS-1序列进行琼脂糖凝胶电泳，能把菌株分类鉴定到属的水平；采用非变性聚丙烯酰胺凝胶电泳能把部分酿酒酵母菌株分类鉴定到种的水平，区别效果较琼脂糖凝胶电泳有所提高；对rDNA ITS-1序列进行克隆、测序，经序列比对后发现，酿酒酵母属中酿酒酵母菌株的ITS-1序列大多数有差异，差异主要表现在ITS-1序列上游的一段Poly-T碱基上，其它处的差异仅是个别碱基的转换或颠换，ITS-1序列长度分布在360-370bp之间，G+C%含量均在35.3%左右。

With the development of lesion inautonomic nervous system, i.e. the elevation of baroreflex set point, blood pressureincreases to stage 1 hypertension and worsens. Furthermore, the role of the effectors,including PVR, ventricular end-systolic elastance, venous unstressed volumes and heartrate, in the regulation of hypertension was analyzed. The results exhibit that the ANSregulation on hypertension works mainly by the reduction of PVR and the increase ofvenous unstressed volume.

随着自主神经系统损坏程度的不断加重，即工作调定点进一步升高，血压水平上升到了1级高血压并趋于严重；研究了神经调节的各效应器(外周血管阻力、心肌最大弹性度、心率和静脉非压力容积)对高血压的作用，表明神经调节机制对高血压的调节主要是通过降低外周血管阻力和增加静脉非压力容积实现的。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Finally, measures and suggestions for solution were proposed aiming at these problems of secondary and primary school teachers in Wuchuan county . 1. Reform of the personnel distribution system and whole staff engagement system should be performed as soon as possible 2. System of selection of high and elementary schoolmasters should be reformed, perfecting schoolmaster responsibility system, reducing organization refinedly, compressing non-teaching personnel proportion, improving administration level of school. 3. Taking powerful measures to stabilize high and elementary school teacher"s team. 4. Executing "the Excellent Teacher Project of Century" to train all teachers, building teachers training mode according to current county"s practices and school"s characteristics to improve teachers" quality. 5. Increasing the adjustment for deployment and optimizing teachers resource configuration to improve the quality of elementary education. 6. Introducing talents actively and strengthening high school teacher team"s construction to satisfy the needs of the development of high school education.7. Strengthening teachers" moral education to improve teachers professional moral level.

最后，针对武川县中小学教师队伍中存在的这些问题，提出了解决的对策与建议：加快人事分配制度改革，全面推行教职工全员聘用制；改革中小学校长选拔任用制度，进一步完善&校长负责制&，精简机构，压缩非教学人员比例，提高学校的管理水平；切实采取各种有力措施，稳定中小学教师队伍；实施&跨世纪园丁&工程，进行全员教师培训，建立符合本县实际和学校特色的教师培训模式，提高教师队伍的整体素质；加大布局调整力度，优化教师资源配置，提高基础教育的质量；积极引进人才，加强高中教师队伍建设，以满足高中教育发展的需求；加强师德教育，提高教师的职业道德水平。

To determine the effects of ovarian steroid hormones and some cytokines on the expression of RANTES in cultured cells METHODS AND RESULTS The study included the following two parts 1 The Protein Production of RANTES in Peritoneal Fluids and the Expression of RANTES mRNA in Eutopic and Ectopic Endometrium of Patients with Endometriosis Twenty-eight patients with endometriosis were selected as study group and twenty patients without endometriosis and maglinant disease were selected as control group . In our study, 10 samples of peritoneal fluid in Em and 10 samples of in En were taken, 13 specimens of ovarian endometrioma , 3 red peritoneal endometriotic lesions , 2 uterosacral ligament nodules , 17 matched eutopic endometrium and 12 specimens of endometrium in En were biopsied.

研究目的 1 从蛋白水平检测RANTES在子宫内膜异位症患者腹水中的表达，从核酸水平比较RANTES在内异症患者的异位病灶和在位内膜与非内异症患者子宫内膜之间表达的差异，探讨RANTES在内异症患者盆腔炎性反应中的作用；2 体外培养在位和异位的子宫内膜细胞，研究细胞因子TNFα、INFγ、雌孕激素以及PPARγ激动剂15-d-PGJ〓对RANTES表达的影响，探讨这些因素在内异症发病机理及内异症患者盆腔炎性反应中的作用。

To resolve this problem, according to the robotic theory, the paper suggests to get the moonquake waves in the horizontal plane coordinate in which the reference direction is the north pole of the moon by coordinate rotation transforming from the moonquake wave measured in the oblique coordinate.

在无人登月探测中，为免使月震计的投放装置过于复杂，也为减小月震计系统的重量、体积和功耗，以及增加系统工作的稳定性和可靠性，不给月震计增加机械的水平和定向调节装置，本文根据机器人理论，通过旋转变换，论证了可以从倾斜、非定向安装的月震计观测到的月震波得到以月球北极为参考方向的水平坐标系的观测，并从实验上作了部分验证，从而为这一难题的解决找到一条可行的探索途径。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。