非毒的

The results of study showed that, the proportion of cotton seed cake could account for 30% in the mixed feed of fish for Cyprinus carp and Ctenopharyngodon idellus, and could reach 50% for Tilapia nilotica.

试验表明，在鲤鱼、草鱼的饲料中，棉籽饼可达30％、尼罗非鲫的可达50％，前者若用0.5％的硫酸亚铁溶液去毒处理，其养鱼效果优于未去毒的，后者可直接饲喂。

Vaccines for BCG, Sabin, measles vaccine, German measles and chicken poxare all active vaccines. Three-in-one vaccines(anti-diphtheria vaccine,pertusis vaccine and 破伤风), and vaccines for encephalitis B, hepatitis B

卡介苗、口服性小儿麻痹疫苗、麻疹、德国麻疹疫苗、和水痘疫苗都是属於活性灭毒的疫苗，而白喉、百日咳、破伤风、日本脑炎、B型肝炎和流行性感冒疫苗则属於非活性疫苗。

In this paper, the complete nucleotide sequence of watermelon mosaic virus Chinese Isolate was determined. On the basis, the HC-Pro protein and CP protein both are the factors for aphid transmission were cloned and expressed in vitro, and their antiserum were prepared. By far western blot, viral receptors were identified in aphids, and their interactions were studied. The molecular mechanism of nonpersistent transmission was further elucidated.

本文完成了我国西瓜花叶病毒的基因组全序列的测定，在此基础上，克隆和体外表达了蚜传因子HC-Pro和CP蛋白，制备了相应抗体，利用Far western blot技术鉴定了其蚜虫体内的受体蛋白，并对三者互作关系进行研究，进一步阐明了非持久性传毒的分子机制。

P. infestans ranks highest in the evolutionary potential and it can easily mutate its Avr genes and thus overccome the only promising solution for late blight control is potato plants carrying corresponding R genes.

致病疫霉菌是一种进化潜力非常高的病原，可以快速突变本身的非毒力基因，因而造成对应的马铃薯抗病基因的失效。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示，重组疫苗接种1周后，免疫鸡产生抗IBV的抗体；而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组；攻毒后，异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势，并且该组低水平CD4+的状态一直持续到试验结束，而其他组CD4+ T淋巴细胞均迅速上升，峰值达到14.5 %；同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达，而其他组攻毒后均无明显变化；保护率结果显示，同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %，与其他各组相比均有显著差异；另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻，其排毒时间和排毒量也均有所减少；强毒攻毒后所有试验组体重无显著差异。

The fermentation rates of mannitol in toxigenic and non-toxigenic El Tor strains of Vibrio cholerae are obviously different,which is a valuable indicator in the rapid identification of toxigenic strain.

产毒和非产毒的El Tor生物型霍乱弧菌对甘露醇发酵利用的速率有明显差别，在霍乱致病株的快速判断中有重要的参考价值。

MTT may be used as a new photosensitizer underlight,non-light and different light energy intensity,with no toxic effectto C6 cells under non-light and increased toxicity under increased lightenergy intensity.

在光与非光、及不同光能量密度条件下应用MTT有可能成为新一代的光敏剂，其在非光照射时对C6无细胞毒作用，其细胞毒作用随光能量密度的增加而增强。

In this study,Using extracted total DNA from duck plague virus vaccine strain infected Chicken embryo fibroblasts cells as template, the 1.0 kb TK gene nonessential for viral replication was amplified by polymerise chain reaction and cloned into T-easy vector to obtain pGTK.

本研究从感染鸡胚成纤维细胞的鸭瘟病毒疫苗毒株中提取了总DNA，并以此为模板，利用PCR技术扩增出病毒生长非必需的TK基因(约1.1kb)，将其克隆入pGEM-T easy载体获得载体pGTK。

The causative agent of the disease is an Corona virus, infectious bronchitis virus IBV）, classified as Corona .Present vaccines for IB are all modified live viruses derived either by sequential passage embrocated chicken eggs. As Immunogenicity of IBV is usually correlated with its virulence, almost all modified live IB vaccines remained insufficient attenuation and have shown a variety of side effects including spread of vaccine virus to no vaccinates, occurrence of long term "carrier" birds, and increasing virulence during in vivo passage.

目前国内外预防本病所用的疫苗大多数是弱毒疫苗，但现用弱毒疫苗都存在以下缺点：（1）与IBV强毒一样，其弱毒疫苗株也能引起潜伏感染，潜伏感染的鸡将终生带毒；（2）疫苗病毒可以由接种鸡传染给非接种鸡，在鸡群之间传播过程中毒力会返强，从而成为新的感染源；（3）传染性支气管炎病毒极易发生变异，且易成为病毒重组的供体和受体，给使用IB传统疫苗预防和根除IB带来极大的困难。

The cell cultures were monitored by provirus DNA PCR, RT-PCR, reverse transcriptase activity assay and real-time RT-PCR, The results of RT activity and DNA PCR, RT-PCR were positive in the supernatant of cell cultures and viral particles were also clearly observed under electron microscope.

本试验基于代次毒分析结果，选取LTR 变异率较高的U3 区，以驴胎皮肤弱毒疫苗感染性分子克隆株pLGFD3-8 和驴强毒株感染性分子克隆株为父本操作系统进行基因替换，构建EIAV 非编码区强弱毒嵌合的全基因分子克隆，将阳性重组质粒命名为pLGFD9-12。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。